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GB/T 27577-2011 English PDF

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GB/T 27577-2011: Determination of vitamin B5 (pantothenic acid) and provitamin B5 (D-pantothenol) in cosmetics -- HPLC/UV and HPLC MS/MS
Status: Valid
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GB/T 27577-2011English329 Add to Cart 3 days [Need to translate] Determination of vitamin B5 (pantothenic acid) and provitamin B5 (D-pantothenol) in cosmetics -- HPLC/UV and HPLC MS/MS Valid GB/T 27577-2011

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Basic data

Standard ID GB/T 27577-2011 (GB/T27577-2011)
Description (Translated English) Determination of vitamin B5 (pantothenic acid) and provitamin B5 (D-pantothenol) in cosmetics -- HPLC/UV and HPLC MS/MS
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 14,110
Date of Issue 2011-12-05
Date of Implementation 2012-03-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from) Announcement of Newly Approved National Standards No. 19 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the cosmetic vitamin B5 (pantothenic acid) and provitamin B5 (D- panthenol) was measured by HPLC UV detection side and HPLC tandem mass spectrometry are two methods. This standard applies to cosmetics, vitamin B5 (pantothenic acid) and provitamin B5 (D- panthenol) quantitative determination, HPLC with UV detection for pantothenic acid, D- panthenol detection limit of 30��g/g, the limit of quantification to 100��g/g. High performance liquid chromatography mass spectrometry for pantothenic acid Shenlian, D- panthenol detection limit of 30ng/g, limit of quantification was 100ng/g.

GB/T 27577-2011: Determination of vitamin B5 (pantothenic acid) and provitamin B5 (D-pantothenol) in cosmetics -- HPLC/UV and HPLC MS/MS


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of vitamin B5 (pantothenic acid) and provitamin B5 (D-afantothenol) in cosmetks. HPLC/UV and HPLC MS/MS ICS 71.100.70 Y42 National Standards of People's Republic of China Cosmetics Vitamin B5 (pantothenic acid) and provitamin B5 (D- panthenol) High performance liquid chromatography ultraviolet Assay and HPLC tandem mass spectrometry DeterminationofvitaminB5 (pantothenicacid) andprovitaminB5 (D-fantothenol) Issued on. 2011-12-05 2012-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized. This standard was drafted. Dalian City Product Quality Supervision, Inspection (National Day of Product Quality Supervision and Inspection Center), Shanghai Daily Chemical Industrial Research Institute (Fragrance Flavor and Cosmetic National Quality Supervision and Inspection Center), Dalian standard detection technology research center. The main drafters of this standard. Maoxi Qin, Hu Xia, Pan Wei, Zheng Shunli, Li Peng, Li Qiong. Cosmetics Vitamin B5 (pantothenic acid) and provitamin B5 (D- panthenol) High performance liquid chromatography ultraviolet Assay and HPLC tandem mass spectrometry

1 Scope

This standard specifies the cosmetics Vitamin B5 (pantothenic acid) and provitamin B5 (D- panthenol) determined by HPLC with UV detection And high performance liquid chromatography tandem mass spectrometry two ways. This standard applies to cosmetics quantitative vitamin B5 (pantothenic acid) and provitamin B5 (D- panthenol) Determination of HPLC UV detection Measurement method of pantothenic acid, D- panthenol detection limit of 30μg/g, the limit of quantification of 100μg/g. High performance liquid chromatography tandem mass spectrometry of pantothenic acid, D- pan Alcohol detection limit of 30ng/g; limit of quantification was 100ng/g.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction Laboratory use specifications and test methods GB/T 6682 Analysis Principle 3 In the two-phase system of water and a water-immiscible organic solvent (such as chloroform or iso-octane, etc.) formed, vitamin B5 (pantothenic acid and Its salts (such as sodium and calcium pantothenate Pan)) and provitamin B5 (D- panthenol) are distributed in the aqueous phase, oil-soluble cosmetic ingredients soluble in organic Phase, and cosmetics are enriched in surfactants at the oil-water interface. You can use two-phase system and vitamin B5 provitamin B5 and Cosmetics and oil-soluble surfactant composition is initially separated potassium ferrocyanide - co-precipitating agent to remove the zinc acetate extract macromolecules group quality. After pantothenic acid under acidic conditions and D- panthenol enriched in the solid phase extraction C18 stationary phase, the removal of other water-soluble interferences, with 40% A Alcohol aqueous solution elution reversed-phase high-performance liquid chromatography, UV detector or a tandem quadrupole mass spectrometry, a standard curve of external standard.

4 Reagents and materials

Unless otherwise indicated, the water is a water GB/T 6682 specified. 4.1 Standard substance English name, CAS number, molecular formula, molecular structure, molecular weight and purity are shown in Table 1. 4.2 Methanol. chromatographically pure. 4.3 methanol. AR. 4.4 formic acid. AR. 4.5 formic acid. Chromatographic pure.

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