|
US$259.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 27518-2011: Diagnostic of west Nile virus infections
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27518-2011 | English | 259 |
Add to Cart
|
3 days [Need to translate]
|
Diagnostic of west Nile virus infections
| Valid |
GB/T 27518-2011
|
PDF similar to GB/T 27518-2011
Basic data | Standard ID | GB/T 27518-2011 (GB/T27518-2011) | | Description (Translated English) | Diagnostic of west Nile virus infections | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 11,181 | | Date of Issue | 2011-11-21 | | Date of Implementation | 2012-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 18 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the West Nile virus reverse transcription polymerase chain reaction (Reverse Transcription. Polymerase Chain Re-action, RT-PCR), plaque reduction neutralization test (Plaque Reduction Neutralization Test, PRNT) and West Nile Virus separation method. This standard applies to susceptible animals detection of West Nile virus disease. | GB/T 27518-2011: Diagnostic of west Nile virus infections---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic of west nile virus infections
ICS 11.220
B41
National Standards of People's Republic of China
West Nile virus disease detection method
Issued on. 2011-11-21
2012-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized.
This standard was drafted. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing hundred Ousai biological engineering technology development center.
The main drafters of this standard. Yangxiu Juan, Sunfu Jun, Dingruo Yu, Tian Yan, Tian Rui, Wang Fei, Yang Jing.
West Nile virus disease detection method
1 Scope
This standard specifies the West Nile virus reverse transcription polymerase chain reaction (ReverseTranscription-PolymeraseChainRe-
action, RT-PCR), plaque reduction neutralization test (PlaqueReductionNeutralizationTest, PRNT) and West Nile virus
Separation method.
This standard applies to the West Nile virus disease in susceptible animals testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Abbreviations
The following abbreviations apply to this document.
ABTSdiammonium2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate). 2,2'- azino - bis - (3-ethylbenzene
Thiazole and dihydro-6-sulfonic acid) diammonium salt
AMV. avian myeloblastosis virus (avianmyeloblastosisvirus)
BSA. bovine serum albumin (bovineserumalbumin)
CPE. cytopathic effect (cytopathiceffect)
cDNA. Complementary DNA (complementaryDNA)
DEPC. Coke ethylene carbonate (diethylpyrocarbonate)
DNAdeoxyribonucleicacid. DNA
dH2O. double distilled water (doubledistiledwater)
dNTP. deoxynucleotide triphosphate (deoxy-ribonucleosidetriphosphate) containing dCTP, dGTP, dATP, dTTP four
Deoxyribonucleoside species
EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraaceticacid)
6-FAM. 6- carboxyfluorescein (6-carboxyfluorescein)
HEPES. hydroxyethyl piperazine acetic sulfuric acid (N-2-hydroxyethylpiperazine-N-ethane-sulphonicacid)
MEM. basal medium (minimaessentialmedium)
OligodT. poly T coupled with a short random bases (few bases) constituting the primer for RT-PCR
PBS. phosphate buffered saline (phosphatebelancedsolution)
PFU. plaque forming units (plaqueformingunits)
PRNT. plaque reduction neutralization test (plaquereductionneutralizationtest)
RNA. ribonucleic acid (ribonucleicacid)
RNasin. Ribonuclease Inhibitor (RNAenzymeinhibitor)
TAE. Acetate EDTA (tris-acetate-EDTATris)
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 27518-2011_English be delivered?Answer: Upon your order, we will start to translate GB/T 27518-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 27518-2011_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 27518-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|