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GB/T 27527-2011 English PDF

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GB/T 27527-2011: Diagnostic techniques for avian encephalomyelitis
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PDF similar to GB/T 27527-2011


Standard similar to GB/T 27527-2011

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Basic data

Standard ID GB/T 27527-2011 (GB/T27527-2011)
Description (Translated English) Diagnostic techniques for avian encephalomyelitis
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 13,171
Date of Issue 2011-11-21
Date of Implementation 2012-03-01
Regulation (derived from) Announcement of Newly Approved National Standards No. 18 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the avian encephalomyelitis diagnostic techniques, including epidemiological, clinical symptoms, histopathological changes, virus isolation, agar immunodiffusion test, neutralization test and the indirect immunofluorescence test. This standard applies to avian encephalomyelitis diagnosis, quarantine and epidemiological investigation.

GB/T 27527-2011: Diagnostic techniques for avian encephalomyelitis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for avian encephalomyelitis ICS 11.220 B41 National Standards of People's Republic of China Avian encephalomyelitis Diagnosis Issued on. 2011-11-21 2012-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. Inner Mongolia Agricultural University, Inner Mongolia Animal Disease Prevention and Control Center, China Animal Disease Control Center, North Beijing Century Technology Co., Ltd. Yuanheng animal epidemic prevention, Yangzhou University, Biological Engineering Co., Ltd. Qingdao-State. The main drafters of this standard. Zhao Z, Zhao effort, Chen Xi Zhao, Sun Ming, Jin-Ling Wang, Qin AJ, Fangen Cheng, Li Ruigang, Wurih rare. Avian encephalomyelitis Diagnosis

1 Scope

This standard specifies the Avian Encephalomyelitis diagnostic techniques, including epidemiology, clinical symptoms, pathological changes, virus isolation, agar Free Immune diffusion test, and test and indirect immunofluorescence assay. This standard applies to the diagnosis, quarantine and epidemiology of avian encephalomyelitis.

2 Acronyms

The following abbreviations apply to this document. AE (V). Avian encephalomyelitis (virus) [avianencephalomyelitis (virus)] AEV-VR. VanRoekel avian strain (AEV standard strain) (avianencephalomyelitisvirus-Van Roekel) EID50. chick embryo half the infective dose (egginfectiousdose) FITC. fluorescein isothiocyanate (fluoresceinisothiocyanate) HE staining. hematoxylin - eosin staining (hematoxylin-eosinstaining) PBS. phosphate buffered saline (phosphatebuffersaline)

3 Epidemiology

In addition to chicken susceptible AEV outside, pheasant, quail, pheasant and turkey can be naturally infected. None of this history and non-immunized chickens Once infected, Chicks incidence is generally 40% to 60%, the mortality rate from 10% to 80% or higher. Chickens of different ages can be infected throughout the year. With Japan Growth of age, chickens AEV resistance growing. Infected chicken eggs and chick embryo offspring can cause the onset of (vertical transmission); with When chickens secretions and excretions can contaminate the environment, feed and water, causing the onset of contact with susceptible chickens (horizontal transmission). Natural Love Under conditions chicks (poults) within 4 weeks of AEV most susceptible; Under laboratory conditions, day-old chicks intracerebral inoculation AEV virulent 5d ~ 7d After the onset can; by embryo infected chicks hatched after the incubation period 1d ~ 7d, oral infection or contact with nature in general after the onset 9d ~ 11d.

4 clinical symptoms

Naturally infected and artificially infected chickens are basically the same clinical symptoms of early depression, lethargy, easy to panic, strabismus, was squat position, head and neck To one side, drinking water, tight legs, splayed or paws curled up, can occur after standing firm, progressive ataxia, head and neck tremors fast Symptoms worse often due to paralysis, failure and death. Adult chickens infection generally does not appear obvious symptoms, sometimes only see mild diarrhea, decreased egg production 10% to 40%, and egg hatchability.

5 Histopathological changes

5.1 Material preparation 5.1.1 Equipment Paraffin section machines, dyeing tanks, slides, cover slips, water bath, incubator, microscope.

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