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GB/T 27521-2011: RT-PCR assay for swine influenza virus nucleic acid
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27521-2011 | English | 229 |
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RT-PCR assay for swine influenza virus nucleic acid
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GB/T 27521-2011
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Basic data | Standard ID | GB/T 27521-2011 (GB/T27521-2011) | | Description (Translated English) | RT-PCR assay for swine influenza virus nucleic acid | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,192 | | Date of Issue | 2011-11-21 | | Date of Implementation | 2012-03-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 18 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the swine influenza virus nucleic acid by RT-PCR method for the technical requirements. This standard specifies the RT-PCR detection method for the detection of porcine lung tissue, nasal and tracheal secretions and cultures of these samples were collected in the swine influenza virus nucleic acid. | GB/T 27521-2011: RT-PCR assay for swine influenza virus nucleic acid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
RT-PCR assay for swine influenza virus nucleic acid
ICS 11.220
B41
National Standards of People's Republic of China
Swine influenza virus nucleic acid by RT-PCR method
Issued on. 2011-11-21
2012-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized.
This standard was drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Chinese Academy of Inspection and Quarantine, China Institute of Veterinary Drug monitoring
The police.
The main drafters of this standard. Qiaozhuan Ling, Han Xueqing, Yanghuan Liang, Liu Ye Bing, Chen Yan, Wu Shaojiang, Chen Hualan, Lin Xiangmei, Xin Xiaoguang, Zhuzhong Wu,
Wang Huiyu, Liu, Li, Mei Lin, Wang Yiqin, Xu Biao, Ruanzhou Xi, Yi Ning Po, such as thin clear, Qin Zhifeng, Lin Zhixiong.
Swine influenza virus nucleic acid by RT-PCR method
1 Scope
This standard specifies the technical requirements for swine influenza virus nucleic acid by RT-PCR methods.
RT-PCR detection method specified in this standard for the detection of swine lung tissue, nasal and tracheal secretions and culture of these samples were collected
It was the swine flu virus nucleic acid.
2 Acronyms
The following abbreviations apply to this document.
DEPC. diethyl pyrocarbonate (diethypyrocarbonate)
dNTP. deoxynucleotide triphosphate (deoxy-ribonucleosidetriphosphate)
EB. ethidium bromide (ethidiumbromide)
M-MLVRT. Moloney murine leukemia virus reverse transcriptase (moloneymurineleukemiavirusreversetran-
scriptase)
PCR. polymerase chain reaction (polymerasechainreaction)
RNA. ribonucleic acid (ribonucleicacid)
RNAaseinhibitor. RNA inhibitors
RT-PCR. reverse transcription - polymerase chain reaction (reverse-transcriptionpolymerasechainreaction)
SPF. specific pathogen free (specificpathogenfree)
TaqDNApolymerase. TaqDNA polymerase
3 Instrument
3.1 PCR instrument.
3.2 low-speed desktop centrifuge.
3.3 electrophoresis.
3.4 electrophoresis tank.
3.5 refrigerator.
3.6 gel imaging system.
3.7 micropipette.
3.8 water bath.
4 Reagents
4.1 LSTRIzolRNA RNA extraction reagents or other commercially available extraction reagent.
4.2 of chloroform, isopropanol, ethanol.
4.3 1.2% agarose gel, A in Appendix A.1.
4.4 50 × TAE buffer, see Appendix A A.2.
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