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GB/T 27517-2011 English PDF

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GB/T 27517-2011: A multiplex RT-PCR method to differentiate the highly pathogenic and classical porcine reproductive and respiratory syndrome virus
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GB/T 27517-2011English259 Add to Cart 3 days [Need to translate] A multiplex RT-PCR method to differentiate the highly pathogenic and classical porcine reproductive and respiratory syndrome virus Valid GB/T 27517-2011

PDF similar to GB/T 27517-2011


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Basic data

Standard ID GB/T 27517-2011 (GB/T27517-2011)
Description (Translated English) A multiplex RT-PCR method to differentiate the highly pathogenic and classical porcine reproductive and respiratory syndrome virus
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 11,164
Date of Issue 2011-11-21
Date of Implementation 2012-03-01
Regulation (derived from) Announcement of Newly Approved National Standards No. 18 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the tests to non- structural protein Nsp2 genomic deletions 30 amino acid coding region is characterized by porcine reproductive and respiratory syndrome virus highly pathogenic strains with non-missing 30 amino acids of porcine reproductive and respiratory syndrome virus strains classic compound RT-PCR differential diagnosis. This standard applies to suspected porcine reproductive and respiratory syndrome virus (PRRSV) infection in swine serum and clinical samples and other samples of the virus nucleic acid detection.

GB/T 27517-2011: A multiplex RT-PCR method to differentiate the highly pathogenic and classical porcine reproductive and respiratory syndrome virus


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
A multiplex RT-PCR method to differentiate the highly pathogenic and classical porcine reproductive and respiratory syndrome virus ICS 11.220 B41 National Standards of People's Republic of China Identification of porcine reproductive and respiratory syndrome virus highly pathogenic Classical strain composite RT-PCR method Issued on. 2011-11-21 2012-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. Henan animal disease prevention and control center. The main drafters of this standard. Wu Zhiming, latent if Yan, Zhang Zhiling, Zhang Jian, Jing Ru top, Liu Guanghui, Zhao Mingjun, Caojie Wei Qian Yong, Chengjun Zhen, Zhao Xueli, Zhang hope. Identification of porcine reproductive and respiratory syndrome virus highly pathogenic Classical strain composite RT-PCR method

1 Scope

This standard specifies the detection of genomic non-structural protein coding region Nsp2 deletion of 30 amino acids characterized by porcine reproductive and respiratory Fully Classic virus strain composite RT-PCR identification of the highly pathogenic strain of the virus co-sign with non-deletion of 30 amino acids of porcine reproductive and respiratory syndrome diagnosis method. This standard applies to suspected infection of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine serum clinical samples and other samples of the virus core Acid test.

2 Reagents and Equipment

Unless otherwise specified, the use of biological and chemical reagents were of analytical grade. RNA extraction reagents used should not be RNA enzymes container Aliquot. 2.1 Reagents 2.1.1 swab suspension (see Appendix A), tissue suspension (see Appendix A), or more agents stored at room temperature. 2.1.2 A's solution (see Appendix A), lysis buffer (TriZol), 1 × TAE DNA electrophoresis buffer (see Appendix A), chloroform, 0.01mol/L (PH7.2) in PBS (see Appendix A), DEPC-treated water (see Appendix A), or more agents at 4 ℃. 2.1.3 isopropanol, 75% ethanol, DNA molecular weight standards, the 6 × sample buffer electrophoresis, or more agents stored at -20 ℃. 2.1.4 positive control, a negative control (see Appendix A). 2.1.5 Composite RT-PCR amplification spend downstream primer sequences see Appendix B. 2.1.6 Identification of porcine reproductive and respiratory syndrome virus highly pathogenic strains of classical composite RT-PCR reaction system composition, description and use Note See Appendix C. 2.2 Equipment PCR amplification, high-speed refrigerated centrifuge (centrifugal force 12000g above), and DNA electrophoresis instrument electrophoresis tank level, temperature water bath, 2 ℃ ~ 8 ℃ refrigerator, -20 ℃ refrigerator, single-channel micropipette (0.5μL ~ 10μL; 2μL ~ 20μL; 20μL ~ 200μL; 100μL ~ 1000μL), tissue homogenizer or mortar, gel imaging system (or UV transmission meter), a vacuum dryer (non-essential). 3 specimen collection, handling, storage and transport Notes 3.1 sample collection and processing of Sampling process can not be cross-contamination of samples, sampling and sample processing should wear disposable gloves, masks, hats. Sample collection and processing 3.2 3.2.1 swab samples 3.2.1.1 Nasal swabs. sampling depth nasal swabs to scrape back and forth 3-5 times, take nasal secretions.

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