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GB/T 25887-2010: Method of PCR-RFLP for detecting complex vertebral malformation in dairy cattle
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 25887-2010 | English | 189 |
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Method of PCR-RFLP for detecting complex vertebral malformation in dairy cattle
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GB/T 25887-2010
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Basic data | Standard ID | GB/T 25887-2010 (GB/T25887-2010) | | Description (Translated English) | Method of PCR-RFLP for detecting complex vertebral malformation in dairy cattle | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B43 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 8,848 | | Date of Issue | 1/10/2011 | | Date of Implementation | 6/1/2011 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Announcement of Newly Approved National Standards 2011 No. (No. 166 overall) 1 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Standard specifies the cow spinal deformity syndrome PCR-RFLP detection technology. This Standard is applicable to the detection and diagnosis of spinal deformity syndrome cows. | GB/T 25887-2010: Method of PCR-RFLP for detecting complex vertebral malformation in dairy cattle---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method of PCR-RFLP for detecting complex vertebral malformation in dairy cattle
ICS 65.020.30
B43
National Standards of People's Republic of China
Cows vertebral malformation syndrome detection PCR-RFLP method
Issued on. 2011-01-10
2011-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix B of this standard is a normative appendix, Appendix A, Appendix C is informative appendix.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee of animal husbandry.
This standard was drafted. Huazhong Agricultural University.
The main drafters of this standard. - selection of Yang Liguo, Li Xiang, Huxiu Zhong, Liu Wenju, Song Lijun.
Cows vertebral malformation syndrome detection PCR-RFLP method
1 Scope
This standard specifies the PCR-RFLP detection cows spinal deformity syndrome.
This standard applies to the detection and diagnosis of spinal deformity Cow Syndrome.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Cows spinal deformity syndrome complexvertebralmalformationindairycattle; CVM
One kind of cow autosomal recessive genetic disease, mainly for cow abortion, early fetal death, died shortly after birth, sick calves
The main symptoms. short neck, spine deformities, heart defects, leg deformities, Department of the Ministry presents symmetrical inward stiff like.
3.2
SLC35A3 gene SLC35A3gene
Located on bovine chromosome 3 in the gene sequence databases (GenBank) the accession number AY160683, full-length 14901bp,
mRNA is 981bp, composed of seven exons and six introns of the gene encoding ribosomal protein transport, and development related to the spine, cows
Spinal deformity syndrome disease gene.
3.3
Restriction fragment length polymorphism restrictionfragmentlengthpolymorphism, RFLP
The size of the detected DNA with the restriction enzyme digestion formed specific DNA segments. Therefore, any change can cause restriction sites
Different mutations such as point mutations (new generation and removal of restriction sites) and reorganize some DNA (such as insertions and deletions cause between restriction sites
The change in length) may also lead to the generation of RFLP.
Principle 4
SLC35A3 gene exon 4 of the first 559 bp G to T, the corresponding encoded amino acid valine change
Phenylalanine, resulting in the occurrence of spinal deformity syndrome cows. Using PCR-RFLP technique to detect bovine genomic DNA sample variation, namely
SLC35A3 gene was amplified by PCR fragment, restriction endonuclease digestion of the PCR amplification products, electrophoresis digestion products of
SLC35A3 gene were classified according to whether they carry the genotype determination cows vertebral malformation syndrome disease gene.
5 SLC35A3 gene amplified fragment introduced restriction site PCR primers
5'CCACTGGAAAAACATGCTGTGAGTA3 '
5'GCTCTCCTCTGTAATCCCCA3 '
Expected amplification length is 225bp.
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