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GB/T 23743-2009: Microbiological examination of the enumeration of coagulase-positive staphylococci in feeds -- Technique using Baird-Parker agar medium
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 23743-2009 | English | 279 |
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Microbiological examination of the enumeration of coagulase-positive staphylococci in feeds -- Technique using Baird-Parker agar medium
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GB/T 23743-2009
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PDF similar to GB/T 23743-2009
Basic data | Standard ID | GB/T 23743-2009 (GB/T23743-2009) | | Description (Translated English) | Microbiological examination of the enumeration of coagulase-positive staphylococci in feeds -- Technique using Baird-Parker agar medium | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 12,164 | | Date of Issue | 2009-05-12 | | Date of Implementation | 2009-09-01 | | Adopted Standard | ISO 6888-1-1999/Amd 1-2003, MOD | | Regulation (derived from) | National Standard Approval Announcement 2009 No.6 (Total No.146) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the coagulase-positive staphylococci in solid medium (Baird-Parker medium), After 36��C �� 1��C under aerobic colony counts cultured technical requirements. This standard applies to feed solidification detect positive staphylococci. | GB/T 23743-2009: Microbiological examination of the enumeration of coagulase-positive staphylococci in feeds -- Technique using Baird-Parker agar medium
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
d) increase in number in the body of the formula.
Appendix A of this standard is an informative annex.
This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) and focal points.
This standard was drafted. Agricultural Quality Standards and Testing Technology Research Institute, Chinese Academy of Agricultural Sciences [National Feed Quality Supervision and Inspection Center
(Beijing)].
The main drafters of this standard. Rao Zhenghua, Lili Bei, Ma Dongxia, Zhang Su, Suxiao Ou.
Positive staphylococci feed solidifying microbiology
Agar counting method
1 Scope
This standard specifies the coagulase-positive staphylococci in solid medium (Baird-Parker medium), 36 ℃ ± 1 ℃ aerobic conditions
After culturing the technical requirements of the colony count.
This standard applies to feed clotting enzyme detected positive staphylococci.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 4789.1 Microbiological examination of food hygiene - General
GB/T 14699.1 feed sampling (GB/T 14699.1-2005, ISO 6497.2002, IDT)
GB/T 20195 preparation of animal feed samples (GB/T 20195-2006, ISO 6498.1998, IDT)
Accuracy (trueness and precision) Part 2 ISO 5725-2 test methods and results. Standard Test Method for determining the repeatability
And basic methods of reproduction
ISO 16140 Microbiology of food and animal feed draft validation of alternative methods
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Under test conditions specified in this standard, cultured in selective solid medium can be formed of typical and/or atypical colonies and plasma coagulation
Enzyme test positive for a group of bacteria.
3.2
According to the method specified in this standard, per milliliter or per gram of sample coagulated number of positive staphylococci.
Principle 4
4.1 quantitatively plated on solid selective medium by surface inoculation, inoculation two plates. Direct inoculation liquid sample, its
He required 1.10 dilution was inoculated samples.
In other dilution, dilution was inoculated with the above method. Two plates inoculated with each dilution.
4.2 Petri dishes at 36 ℃ ± 1 ℃ aerobic culture, the culture 24h and 48h time have to be checked.
4.3 Select the appropriate dilution level, the number of colonies grown dish, per gram (per milliliter) sample contains coagulase-positive grapes
The number of bacteria.
5 Diluent and culture medium
5.1 General
Unless otherwise indicated in the analysis using only recognized as analytical reagents, laboratory water using distilled or deionized water or substantially pure
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