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GB/T 23737-2009 English PDF

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GB/T 23737-2009: Determination of free canavanine in feeds -- Ion exchange chromatography
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Basic data

Standard ID GB/T 23737-2009 (GB/T23737-2009)
Description (Translated English) Determination of free canavanine in feeds -- Ion exchange chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B46
Classification of International Standard 65.120
Word Count Estimation 8,860
Date of Issue 2009-05-12
Date of Implementation 2009-09-01
Quoted Standard GB/T 603; GB/T 6682; GB/T 14699.1; GB/T 20195
Regulation (derived from) PRC National Standard Approval Announcement 2009 No.6 (Total No.146)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the determination of free feed canavanine content by ion-exchange chromatography. This standard applies to a single feed and feed the free determination of canavanine. Limit of quantification was 15mg/kg.

GB/T 23737-2009: Determination of free canavanine in feeds -- Ion exchange chromatography

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Determination of free canavanine in feeds. Ion exchange chromatography ICS 65.120 B46 National Standards of People's Republic of China Feed the free determination of canavanine Ion exchange chromatography Posted 2009-05-12 2009-09-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the National Standardization Technical Committee Feed Industry and focal points. This standard was drafted. Beijing Institute of Animal Chinese Academy of Agricultural Sciences, Chinese Academy of Agricultural Sciences Agricultural Quality Standards and Testing Technology Institute [National Feed Quality Supervision and Inspection Center (Beijing)]. The main drafters of this standard. Tong Jianming, Wu Yingying, Dong Xiaofang, Zhang Qi, Suxiao Ou. Feed the free determination of canavanine Ion exchange chromatography

1 Scope

This standard specifies the determination of free feed canavanine content by ion exchange chromatography. This standard applies to single feed and feed of free determination of canavanine. Limit of quantification is 15mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 603 chemical reagent in test methods - Preparations with Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples GB/T 20195 Animal feed sample preparation Principle 3 Free sample canavanine by Sulfosalicyl shaking extraction, filtration and volume. The extract was separated and the ion exchange column In after post-column ninhydrin indene derivatives measured at a wavelength of 570nm.

4 Reagents and solutions

Except where noted, this standard reagents used were of analytical grade, water accord GB/T 6682 in two water provision, water accord chromatography GB/T 6682 regulations in a water solution formulation according to GB/T 603. 4.1 hydrochloric acid solution. Pipette 1.7mL of concentrated hydrochloric acid was diluted with water to 1L. 4.2 sulfosalicylic acid solution. 50g/L. Take 5.0g Sulfosalicyl diluted with water to 100mL. 4.3 the different pH and ionic strength of the elution with sodium citrate buffer. prepared according to instrument manual. 4.4 ninhydrin solution. according to instrument manual preparation. 4.5 canavanine (C5H12N4O3) Standard. Purity ≥98%. 4.6 canavanine standard solution. Weigh 0.001g canavanine, accurate to 0.0001g, dissolved in hydrochloric acid solution (4.1) and dilute to 10mL, the concentration of 100μg/mL, at 4 ℃ refrigerator. 4.7 canavanine working standard solution. Pipette canavanine standard solution (4.6) 0.1mL, 0.2mL, 0.5mL, 1.0mL, 2.0mL, 4.0mL to 10mL volumetric flask with hydrochloric acid solution (4.1) and dilute to 10mL, mix formulated to 1.0μg/mL, 2.0μg/mL, 5.0μg/mL, 10.0μg/mL, 20.0μg/mL, 40.0μg/mL of canavanine standard working solution. Using now. 5. Apparatus 5.1 rotary evaporator. 5.2 laboratory sample grinder. 5.3 Sample sieve. aperture 0.25mm.

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