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GB/T 23380-2009 English PDF

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GB/T 23380-2009: Determination of carbendazim residues in fruits and vegetables -- HPLC method
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GB/T 23380-2009English139 Add to Cart 3 days [Need to translate] Determination of carbendazim residues in fruits and vegetables -- HPLC method Valid GB/T 23380-2009

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Basic data

Standard ID GB/T 23380-2009 (GB/T23380-2009)
Description (Translated English) Determination of carbendazim residues in fruits and vegetables -- HPLC method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 6,679
Date of Issue 2009-04-08
Date of Implementation 2009-05-01
Regulation (derived from) National Standard Approval Announcement 2009 No.3 (Total No.143)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the fruit, performance liquid chromatographic method carbendazim residues in vegetables. This standard applies to fruit, Determination of carbendazim residues in vegetables. This standard method detection limit: 0. 02mg/kg.

GB/T 23380-2009: Determination of carbendazim residues in fruits and vegetables -- HPLC method

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Determination of carbendazim residues in fruits and vegetables.HPLC method ICS 67.050 X04 National Standards of People's Republic of China Determination of fruits, vegetables carbendazim residues High Performance Liquid Chromatography Posted 2009-04-08 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. The standard proposed by the Anhui Provincial Quality and Technical Supervision. The standard by the China National Institute of Standardization. This standard was drafted. National Agricultural Processed Food Quality Supervision and Inspection Center, Anhui national agricultural standardization and monitoring center. The main drafters of this standard. Nie Lei, Lu industry move, Shao Dongliang, Zhang Bo, Zhang Xian Ling, Zhao Vic, as Yao Yan. Determination of fruits, vegetables carbendazim residues High Performance Liquid Chromatography

1 Scope

This standard specifies a high performance liquid chromatographic method in fruits and vegetables carbendazim residues. This standard applies to the determination of fruits and vegetables carbendazim residues. The standard method detection limit. 0.02mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) GB/T 8855 fresh fruits and vegetables sampling method (GB/T 8855-2008, ISO 874. 1980, IDT) Principle 3 Fruit, vegetable samples carbendazim by accelerated solvent extraction instrument (ASE), and the extract was dried solid phase extraction (SPE) separation, purification, concentration Shrink, detected by HPLC, external standard after volume.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade, test water are a water GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 0.1mol/L hydrochloric acid. 4.3 2% aqueous ammonia (volume fraction). 2mL aqueous ammonia (25% ~ 28%) + 98mL water. 4.4 2% ammonia - methanol solution (volume fraction). 2mL aqueous ammonia (25% ~ 28%) + 98mL methanol. 4.5 4% ammonia - methanol solution (volume fraction). 4mL aqueous ammonia (25% ~ 28%) + 96mL methanol. 4.6 phosphate buffer solution (0.02mol/L, pH = 6.8). 1.38g sodium dihydrogen phosphate 1.41g disodium hydrogen phosphate and dissolved in 900mL Water, adjusted to pH 6.8 with phosphoric acid, dilute to 1000mL. 4.7 Solid phase extraction column (OasisMCX6mL, 150mg, or equivalent person), before use successively with 2mL methanol, 3mL2% aqueous ammonia Activated. 4.8 carbendazim standard solution. 100μg/mL. Cold light. 4.9 carbendazim standard solution. Take the above standard solution with a series of work needed to match the flow made the appropriate concentration of the standard solution, Now with the current need. 5. Apparatus 5.1 liquid chromatograph. with diode array detector (DAD) or UV detector (UV). 5.2 Accelerated Solvent Extractor (ASE). Extraction reference conditions. 34mL extraction cell, temperature 100 ℃, pressure 13.80MPa (2000psi), plus Heat 5min, using methanol as solvent static extraction 5min, 60% solvent quick rinse samples, 60s nitrogen purge. 5.3 Solid Phase Extraction System (SPE).

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