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GB/T 23381-2009 English PDF

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GB/T 23381-2009: Determination of 6-benzylaminopurine in foods -- High-performance liquid chromatography
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GB/T 23381-2009English139 Add to Cart 3 days [Need to translate] Determination of 6-benzylaminopurine in foods -- High-performance liquid chromatography Valid GB/T 23381-2009

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Basic data

Standard ID GB/T 23381-2009 (GB/T23381-2009)
Description (Translated English) Determination of 6-benzylaminopurine in foods -- High-performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 6,650
Date of Issue 2009-04-08
Date of Implementation 2009-05-01
Quoted Standard GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2009 No.3 (Total No.143)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the determination in food by high performance liquid chromatography 6-Method benzyladenine (6-BA) content. This standard applies to fruits and vegetables (bean sprouts, cucumber, tomato, mushrooms, strawberries, orange), and other plant foods and products 6-benzyladenine determination.

GB/T 23381-2009: Determination of 6-benzylaminopurine in foods -- High-performance liquid chromatography


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Determination of 6 -benzylaminopurine in foods.High-performance liquid chromatography ICS 67.050 X04 National Standards of People's Republic of China Determination of 6-benzyladenine High Performance Liquid Chromatography Posted 2009-04-08 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the National Food Industry Standardization Technical Committee proposed. This standard by the National Standardization Technical Committee on Food Universal Detection Technology at the Technical Committee on Food industry. Drafting of this standard. Shanghai Quality Supervision, Inspection Technology Research Institute, the National Food Quality Supervision and Inspection Center (Shanghai). The main drafters of this standard. Duan Wen-feng, Lin Lin, Xiong Wei, Cao Chengming. Determination of 6-benzyladenine High Performance Liquid Chromatography

1 Scope

This standard specifies the method for Determination of 6-benzyladenine (6-BA) content by HPLC. This standard applies to fruits and vegetables (bean sprouts, cucumber, tomato, mushrooms, strawberries, orange), and other plant foods and products of 6-benzyladenine Determination.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

3 Method summary

Samples were extracted with methanol, concentrated and purified by HPLC, external standard.

4 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 glacial acetic acid. 4.3 6-benzyl adenine standards. purity ≥99.0%. 4.4 C18 solid phase extraction column. 6mL, 500mg, or rather who, prior to use successively with methanol 5mL, 10mL water activation. 4.5 ammonium acetate solution (0.02mol/L). Weigh 1.54g ammonium acetate, with the amount of water dissolved, 1.0mL of glacial acetic acid, add water to 1000mL. 4.6 6-benzyl adenine standard stock solution. Weigh accurately 6-benzyl adenine standards 0.0100g to 100mL volumetric flask, with methyl Alcohol and dissolve to volume. This solution corresponds to 1.0mL 0.10mg6- benzylaminopurine. 4.7 6-benzyl adenine standard working solution. take the 6-benzyl adenine standard stock solution 1.0mL, diluted with methanol and set the volume to 100mL, this Solution 1.0mL equivalent 0.0010mg6- benzylaminopurine. 4.8 microporous membrane. 0.45μm, organic phase. 5. Apparatus 5.1 Organization mashed machines. 5.2 centrifuge. speed of not less than 4000r/min. 5.3 ultrasonic cleaning. 5.4 rotary evaporator. 5.5 High Performance Liquid Chromatography (HPLC) instrument. with a UV detector or diode array detector. 5.6 Solid phase extraction device. 5.7 Electronic balance. a sense of the amount of 0.1mg.

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