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GB/T 21637-2008: Method of morphological identification of coronavirus by using transmission electron microscopy
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Method of morphological identification of coronavirus by using transmission electron microscopy
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GB/T 21637-2008
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Basic data | Standard ID | GB/T 21637-2008 (GB/T21637-2008) | | Description (Translated English) | Method of morphological identification of coronavirus by using transmission electron microscopy | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | N53 | | Classification of International Standard | 11.100; 71.040.99 | | Word Count Estimation | 14,161 | | Date of Issue | 2008-04-11 | | Date of Implementation | 2008-10-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the application of transmission electron microscopy methods and identification of coronavirus, requirements and coronavirus morphology and morphogenesis feature. This standard applies to the use of transmission electron microscopy coronavirus morphological identification. | GB/T 21637-2008: Method of morphological identification of coronavirus by using transmission electron microscopy
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Method of morphological identification of coronavirus by using transmission electron microscopy
ICS 11.100; 71.040.99
N53
National Standards of People's Republic of China
Coronavirus transmission electron microscopy
Morphological identification method
Posted 2008-04-11
2008-10-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard and Appendix B is an informative annex.
This standard by the National Standardization Technical Committee microbeam analysis and focal points.
This standard was drafted. Institute of Geology and Geophysics, Chinese Academy of Military Medical Sciences Basic Medicine
Institute, the National Center of Biomedical Analysis, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Second Military Medical University foundation
Department of Fudan University Shanghai Medical College, Guangzhou Xing Shi-yuan Biological Technology Co., Ltd. Photonic Microsystems.
The main drafters of this standard. Chen Dehui, Zeng Rong tree, Yang Yi, Liu Shide, Yong Ji, Yu Chang, Bi Shu.
Introduction
With the development of electron microscopy, and in vitro virus virus separation and purification technology, the virus morphology, chemical composition, genetic structure
Increasing knowledge of biological characteristics as well as its other aspects. According to virus transmission electron microscope morphological characteristics. virion
Shape and size, and the capsid symmetry (symmetry or helical symmetry is a perspective) and the size, number and arrangement of shell particles, in a host of fine nucleocapsid
Intracellular replication and assembly sites, with or without an envelope, the envelope surface of the spike of the size, shape and spacing, and enveloped virus budding maturity site
Etc., combined with the virus biology and molecular characterization of nucleic acids and proteins of the virus classification and nomenclature. Known people
Class of pathogenic viruses involved in at least 22 families (Family) and two unclassified virus genus (Genus). Virus taxonomy and nomenclature
Morphological characteristics of the virus is the primary indication. Practice has proved that, according to virus morphology and morphogenesis characteristics can be applied quasi-electron microscopy
Correctly identified the virus to families. In order to properly guide diagnosis and appraisal of human viral infectious viral pathogens, it is necessary to develop human
Disease-associated virus transmission electron microscope morphological analysis Identification of national standards.
Coronavirus transmission electron microscopy
Morphological identification method
1 Scope
This standard specifies the electron microscope observation and identification of coronavirus transmission method of application, requirements and coronavirus morphology and shape hair
Health characteristics (see Appendix A, Appendix B).
This standard applies to the use of a transmission electron microscope identification of the coronavirus morphology.
2 Terms and Definitions
The following terms and definitions apply to this standard.
2.1
A class of relatively primitive, with only one nucleic acid (DNA or RNA), capable of self-replication and cell parasitic within strict non-cellular organisms.
2.2
Copy, fully assembled mature viral particles.
2.3
Capsid proteins and nucleic acids by the composition of the center of the virus, there are two kinds of three-dimensional symmetry and helical symmetry.
2.4
Protein subunits are multiplexed nucleic acid wound on the outside, along the central axis was spirally arranged, the formation of highly ordered, symmetrical stable structure.
2.5
Outside the viral nucleocapsid by lipoprotein and mucin consisting of lipid bilayer membranes.
2.6
The outer envelope spike glycoprotein composed of projections having antigenicity.
2.7
High-density heavy metal salts (such as phosphotungstic acid, uranyl acetate, etc.) dye, will be surrounded by a tiny virus, the virus is displayed on a dark background
Fine structure.
2.8
Ultra-thin slicer fixed biological sample after embedding, cut into thin slices having a thickness of 60nm ~ 80nm for TEM
Observed.
3 equipment and materials
3.1 The main equipment
a) transmission electron microscope;
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