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GB/T 18654.10-2026: Inspection of germplasm for fishes - Part 10: Determination on nutritional component of muscle
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GB/T 18654.10: Evolution and historical versions
| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| GB/T 18654.10-2026 | English | 219 |
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Inspection of germplasm for fishes - Part 10: Determination on nutritional component of muscle
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| GB/T 18654.10-2008 | English | 149 |
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Inspection of germplasm for cultured fishes -- Part 10: Determination on nutrient content of muscle
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| GB/T 18654.10-2002 | English | 239 |
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Inspection of germplasm for cultured fishes -- Part 10: Determination on nutrient content of muscle
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Basic data | Standard ID | GB/T 18654.10-2026 (GB/T18654.10-2026) | | Description (Translated English) | Inspection of germplasm for fishes - Part 10: Determination on nutritional component of muscle | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B50 | | Classification of International Standard | 65.150 | | Word Count Estimation | 10,129 | | Date of Issue | 2026-02-27 | | Date of Implementation | 2026-09-01 | | Older Standard (superseded by this standard) | GB/T 18654.10-2008, GB/T 18654.11-2008 | | Issuing agency(ies) | State Administration for Market Regulation, Standardization Administration of China | GB/T 18654.10-2026: Inspection of germplasm for fishes - Part 10: Determination on nutritional component of muscle
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.150
CCSB50
National Standards of the People's Republic of China
Replaces GB/T 18654.10-2008 and GB/T 18654.11-2008
Fish germplasm testing
Part 10.Determination of Muscle Nutritional Components
Published on 2026-02-27
Implemented on 2026-09-01
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
This document is Part 10 of GB/T 18654, "Fish Germplasm Inspection". GB/T 18654 has already published the following parts.
---Part 1.Inspection Rules;
---Part 2.Sampling Methods;
---Part 3.Characteristic Determination;
---Part 4.Measurement of Age and Growth;
---Part 5.Dietary Analysis;
---Part 6.Determination of reproductive performance;
---Part 7.Ecological Characteristics Analysis;
---Part 8.Determination of Oxygen Consumption Rate and Critical Asphyxia Point;
---Part 9.Meat Percentage Determination;
---Part 10.Determination of muscle nutrient composition;
---Part 12.Karyotype Analysis;
---Part 13.Isozyme Electrophoresis Analysis;
---Part 14.Determination of DNA content.
This document supersedes GB/T 18654.10-2008 "Geological Examination of Cultured Fish - Part 10.Determination of Muscle Nutritional Components" and
GB/T 18654.11-2008 "Genomic Testing of Cultured Fish - Part 11.Determination of Major Amino Acid Content in Muscle". This document is based on...
Based on GB/T 18654.10-2008, this standard integrates the content of GB/T 18654.11-2008.Compared with GB/T 18654.10-2008, it includes the following.
Aside from structural adjustments and editorial changes, the main technical changes are as follows.
a) The calculation of nitrogen-free leachate has been removed (see 6.6 in the.2008 edition);
b) The contents of the instruments and equipment have been changed (see 5.3, 3.3 in the.2008 edition);
c) The sample processing procedures have been changed (see Chapter 6, Chapter 5 of the.2008 edition);
d) The methods for determining moisture, ash, protein, fat, and fatty acids have been revised (see 7.1–7.4, 7.6, and 6.1–6.5 in the.2008 edition);
e) Reagents and materials have been added (see Chapter 4);
f) A freeze-drying method for moisture determination has been added (see 7.1.2);
g) Added a method for the determination of amino acids (see 7.5);
h) Added data processing (see Chapter 8).
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Fisheries Standardization (SAC/TC156).
This document was drafted by. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences; Shanghai Ocean University; Institute of Hydrobiology, Chinese Academy of Sciences.
Guangxi Zhuang Autonomous Region Fisheries Research Institute.
The main drafters of this document are. Jiang Ming, Dong Lixue, Zhou Ruiqiong, Zhao Jinliang, Xie Shouqi, Luo Yongju, Wen Hua, Tian Juan, Yu Yali, Huang Ting, and Chen Liting.
Yan Xin.
The release history of this document and the document it replaces is as follows.
---First published in.2002 as GB/T 18654.10-2002, and revised for the first time in.2008;
---This is the second revision, incorporating content from 18654.11-2008.
Introduction
Aquatic germplasm resources are an important material basis for fisheries production activities. The "Genomic Testing of Cultured Fish" series of standards is a set of standards for fish germplasm testing.
Fundamental and general standards. Since their implementation, these standards have played a vital role in fish resource surveys, conservation, propagation, and utilization. To better...
To better serve the needs of fisheries development and testing, the name of "Fish Germplasm Inspection" has been changed to "Fish Germplasm Inspection," and some content of the standard has been revised.
It has been revised.
GB/T 18654 is proposed to consist of the following parts.
---Part 1.Inspection Rules. This section describes the classification, inspection items, judgment rules, and re-inspection rules for fish germplasm testing.
---Part 2.Sampling Methods. The purpose is to describe the reagents and equipment, sampling rules, and sampling procedures used in fish germplasm testing.
Order, sample size, and sample preservation.
---Part 3.Trait Determination. The purpose is to describe the reagents and materials, instruments and equipment, sample preparation, and tests used in the determination of fish traits.
Steps and data processing.
---Part 4.Determination of Age and Growth. The purpose is to describe the reagents, materials, instruments, and equipment used in determining the age and growth of fish.
Samples, test procedures, and data processing.
---Part 5.Feeding Analysis. The purpose is to describe the reagents and materials, instruments and equipment, samples, experimental procedures, and methods used in fish feeding analysis.
Data processing.
---Part 6.Determination of Reproductive Performance. The purpose is to describe the reagents, materials, instruments, and equipment used in determining spawning and egg production in fish.
Samples, test procedures, and data processing.
---Part 7.Ecological Characteristics Analysis. This section describes the reagents and instruments used to analyze the ecological characteristics (temperature, salinity tolerance) of fish.
Equipment, samples, test procedures, and data processing.
---Part 8.Determination of Oxygen Consumption Rate and Critical Asphyxiation Point in Fish. The purpose is to describe the reagents used in determining oxygen consumption rate and critical asphyxiation point in fish.
Materials, instruments and equipment, samples, test procedures and data processing.
---Part 9.Determination of Flesh Percentage. The purpose is to describe the reagents and materials, instruments and equipment, samples, and experimental procedures used in the determination of flesh percentage in fish.
Steps and data processing.
---Part 10.Determination of Muscle Nutritional Components. The purpose is to describe the nutritional components of fish muscle (moisture, ash, protein, amino acids).
Reagents and materials, instruments and equipment, samples, test procedures and data processing for the determination of acids, fats and fatty acids.
---Part 12.Karyotype Analysis. The purpose is to describe the principles, reagents and materials, and instruments used in fish karyotype analysis.
Preparation, samples, test procedures and data processing.
---Part 13.Isozyme Electrophoresis Analysis. The purpose is to describe the reagents, materials, instruments, and samples used in isozyme electrophoresis analysis of fish.
Products, testing procedures, and data processing.
---Part 14.Determination of DNA Content. The purpose is to describe the reagents, materials, instruments, and equipment used in the determination of DNA content in fish.
Samples, test procedures, and data processing.
Fish germplasm testing
Part 10.Determination of Muscle Nutritional Components
1 Scope
This document describes the reagents and materials, instruments and equipment, samples, test procedures and data processing for the determination of nutritional components in fish muscle.
This document applies to the determination of nutritional components in fish muscle.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
GB 5009.3-2016 National Food Safety Standard - Determination of Moisture in Food
GB 5009.4-2016 National Food Safety Standard. Determination of Ash Content in Food
GB 5009.5-2025 National Food Safety Standard - Determination of Protein in Food
GB 5009.6-2016 National Food Safety Standard - Determination of Fat in Food
GB 5009.124-2016 National Food Safety Standard - Determination of Amino Acids in Food
GB 5009.168-2016 National Food Safety Standard - Determination of Fatty Acids in Food
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 18654.2 Fish germplasm testing – Part 2.Sampling methods
3 Terms and Definitions
This document does not contain any terms or definitions that need to be defined.
4.Reagents and Materials
Unless otherwise specified, all reagents used in this method are of analytical grade, and the water is grade III water as specified in GB/T 6682.Other reagents and materials are as follows.
GB 5009.3-2016, GB 5009.4-2016, GB 5009.5-2025, GB 5009.6-2016, GB 5009.124-2016,
The provisions of GB 5009.168-2016.
5.Instruments and Equipment
5.1 Fish dissection tools and routine laboratory equipment.
5.2 Weighing bottle.
5.3 Electronic balance. sensitivity is 0.001g and 0.1g respectively.
5.4 Electric heating constant temperature drying oven.
5.5 Freeze dryer.
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