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GB/T 18654.12-2026: Inspection of germplasm for fishes - Part 12: Test methods for the karyotype
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GB/T 18654.12: Evolution and historical versions
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| GB/T 18654.12-2026 | English | 299 |
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Inspection of germplasm for fishes - Part 12: Test methods for the karyotype
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| GB/T 18654.12-2008 | English | 229 |
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Inspection of germplasm for cultured fishes -- Part 12: Method for the Karyotype analysis
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| GB/T 18654.12-2002 | English | 399 |
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Inspection of germplasm for cultured fishes -- Part 12: Method for the karyotype analysis
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Basic data | Standard ID | GB/T 18654.12-2026 (GB/T18654.12-2026) | | Description (Translated English) | Inspection of germplasm for fishes - Part 12: Test methods for the karyotype | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B50 | | Classification of International Standard | 65.150 | | Word Count Estimation | 14,133 | | Date of Issue | 2026-02-27 | | Date of Implementation | 2026-09-01 | | Older Standard (superseded by this standard) | GB/T 18654.12-2008 | | Issuing agency(ies) | State Administration for Market Regulation, Standardization Administration of China | GB/T 18654.12-2026: Inspection of germplasm for fishes - Part 12: Test methods for the karyotype---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.150
CCSB50
National Standards of the People's Republic of China
Replaces GB/T 18654.12-2008
Fish germplasm testing
Part 12.Chromosome Karyotype Analysis
Published on 2026-02-27
Implemented on 2026-09-01
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
This document is Part 12 of GB/T 18654, "Fish Germplasm Inspection". GB/T 18654 has already published the following parts.
---Part 1.Inspection Rules;
---Part 2.Sampling Methods;
---Part 3.Characteristic Determination;
---Part 4.Measurement of Age and Growth;
---Part 5.Dietary Analysis;
---Part 6.Determination of reproductive performance;
---Part 7.Ecological Characteristics Analysis;
---Part 8.Determination of Oxygen Consumption Rate and Critical Asphyxia Point;
---Part 9.Meat Percentage Determination;
---Part 10.Determination of muscle nutrient composition;
---Part 12.Karyotype Analysis;
---Part 13.Isozyme Electrophoresis Analysis;
---Part 14.Determination of DNA content.
This document replaces GB/T 18654.12-2008 "Germany quality testing of farmed fish - Part 12.Karyotype analysis", and is consistent with...
Compared with GB/T 18654.12-2008, apart from structural adjustments and editorial changes, the main technical changes are as follows.
a) Reagents and materials have been changed (see Chapter 5, Chapter 5 of the.2008 edition);
b) The instruments and equipment were changed (see Chapter 6, Chapter 6 of the.2008 edition);
c) Change "sampling" to "sample" (see Chapter 7, 7.1 of the.2008 edition);
d) Change "Preparation of glass slide specimens" to "Experimental procedures" (see Chapter 8, Chapter 7 of the.2008 edition);
e) Improved the preservation of slide specimens (see 8.2);
f) Microscopic observation and photography have been added (see 8.3);
g) Change "Match Analysis" to "Data Processing" (see Chapter 9, Chapter 8 in the.2008 edition);
h) The method of measuring chromosome arm count using vernier calipers has been removed (see 8.3 in the.2008 edition);
i) Added a method for measuring chromosome length using Photoshop or similar software (see 9.3);
j) The formulation of the karyotype formula has been changed (see 9.4, 8.4 in the.2008 edition).
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Fisheries Standardization (SAC/TC156).
This document was drafted by. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences; Guangxi Zhuang Autonomous Region Fisheries Research Institute; and Zhejiang Province.
Institute of Hydrobiology, Chinese Academy of Agricultural Sciences; Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences.
The main drafters of this document are. Zhou Jianguang, Luo Yongju, Lou Bao, Zhang Lin, Zhou Yuntao, Li Chitao, Mao Tao, Liu Feng, Lin Yong, Yu Yali, and Gu Wei.
Zhang Tao, Peng Jie, Zhang Lang, Xu Gefeng, Yan Xin, Huang Ting, Liu Yongtao, Wu Gang, Dong Lixue, Lu Zhuanling, Wu Tiejun, Luo Bang, He Li, Li Zhen.
The release history of this document and the document it replaces is as follows.
---First published in.2002 as GB/T 18654.12-2002, and revised for the first time in.2008;
---This is the second revision.
Introduction
Aquatic germplasm resources are an important material basis for fisheries production activities. The "Genomic Testing of Cultured Fish" standard includes sections on fish germplasm testing.
This is a fundamental and universal standard. Since its implementation, the standard has played an important role in fish resource surveys, conservation, propagation, and utilization. To further...
To better serve the needs of fisheries development and testing, the name of "Cultivated Fish Germplasm Inspection" has been changed to "Fish Germplasm Inspection," and some parts of the standard have been revised accordingly.
The content has been revised.
GB/T 18654 is proposed to consist of the following parts.
---Part 1.Inspection Rules. This section describes the classification, inspection items, judgment rules, and re-inspection rules for fish germplasm testing.
---Part 2.Sampling Methods. The purpose is to describe the reagents and equipment, sampling rules, and sampling procedures used in fish germplasm testing.
Order, sample size, and sample preservation.
---Part 3.Trait Determination. The purpose is to describe the reagents and materials, instruments and equipment, sample preparation, and tests used in the determination of fish traits.
Steps and data processing.
---Part 4.Determination of Age and Growth. The purpose is to describe the reagents, materials, instruments, and equipment used in determining the age and growth of fish.
Samples, test procedures, and data processing.
---Part 5.Feeding Analysis. The purpose is to describe the reagents and materials, instruments and equipment, samples, experimental procedures, and methods used in fish feeding analysis.
Data processing.
---Part 6.Determination of Reproductive Performance. The purpose is to describe the reagents, materials, instruments, and equipment used in determining egg-bearing and spawning rates in fish.
Samples, test procedures, and data processing.
---Part 7.Ecological Characteristics Analysis. This section describes the reagents and instruments used to analyze the ecological characteristics (temperature, salinity tolerance) of fish.
Equipment, samples, test procedures, and data processing.
---Part 8.Determination of Oxygen Consumption Rate and Critical Asphyxiation Point in Fish. The purpose is to describe the reagents used in determining oxygen consumption rate and critical asphyxiation point in fish.
Materials, instruments and equipment, samples, test procedures and data processing.
---Part 9.Determination of Flesh Percentage. The purpose is to describe the reagents and materials, instruments and equipment, samples, and experimental procedures used in the determination of flesh percentage in fish.
Steps and data processing.
---Part 10.Determination of Muscle Nutritional Components. The purpose is to describe the nutritional components of fish muscle (moisture, ash, protein, amino acids).
Reagents and materials, instruments and equipment, samples, test procedures and data processing for the determination of acids, fats and fatty acids.
---Part 12.Karyotype Analysis. The purpose is to describe the principles, reagents and materials, and instruments used in fish karyotype analysis.
Preparation, samples, test procedures and data processing.
---Part 13.Isozyme Electrophoresis Analysis. The purpose is to describe the reagents, materials, instruments, and samples used in isozyme electrophoresis analysis of fish.
Product, testing procedures and data processing.
---Part 14.Determination of DNA Content. The purpose is to describe the reagents, materials, instruments, and equipment used in the determination of DNA content in fish.
Samples, test procedures, and data processing.
Fish germplasm testing
Part 12.Chromosome Karyotype Analysis
1 Scope
This document describes the principles, reagents and materials, instruments and equipment, samples, experimental procedures, and data processing for fish karyotype analysis.
This document applies to karyotype analysis for fish germplasm testing.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are listed below.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 18654.2 Fish germplasm testing – Part 2.Sampling methods
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Under aseptic conditions, kidney, spleen, blood, or epithelial tissue cells from fish are obtained, and cell division stimulants are added during in vitro culture.
The drug induces a large number of lymphocytes to enter a state of division; then colchicine is added to inhibit cell division at metaphase, thus obtaining fish cells.
Metaphase of chromosome division.
3.2
Injecting cell division stimulants into the fish to induce a large number of lymphocytes to enter a state of division, or injecting colchicine (or removing kidney tissue)
Fish cell staining was obtained by thoroughly tearing up tissue or spleen tissue in physiological saline and adding colchicine to inhibit cell division at metaphase.
Metaphase of chromosomal splitting.
3.3
The sample fish were temporarily housed in a colchicine solution, which inhibited the division of their rapidly dividing gill or fin epithelial cells.
During this period, the metaphase phase of fish cell chromosomes was obtained.
3.4
Select normally developing blastocysts, early gastrulation embryos, or eyed embryos, disperse their cells by pipetting, and add colchicine to make the cells...
Cell division is inhibited in metaphase, resulting in the metaphase phase of fish cell chromosomes.
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