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GB 31636-2016: Pine pollen
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| GB 31636-2016 | English | 179 |
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Pine pollen
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GB 31636-2016
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Basic data
| Standard ID | GB 31636-2016 (GB31636-2016) |
| Description (Translated English) | Pine pollen |
| Sector / Industry | National Standard |
| Classification of Chinese Standard | X83 |
| Word Count Estimation | 9,984 |
| Date of Issue | 2016-12-23 |
| Date of Implementation | 2017-06-23 |
| Older Standard (superseded by this standard) | GH/T 1030-2004 Partial |
| Regulation (derived from) | National Health and Family Planning Commission Notice No.17 of 2016 |
| Issuing agency(ies) | National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration |
GB 31636-2016: Pine pollen
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
2.1.1 bee pollen
Worker bees collecting pollen.
2.1.1.1 single species of bee pollen
Worker bees collecting pollen of a plant pollen formation.
2.1.1.2 miscellaneous pollen
Worker bees collect pollen two kinds or more of bee pollen plants formed, and mixtures of two or more than two single bee pollen species.
2.1.1.3 broken bee pollen
Bee pollen powder agglomerates formed after crushing.
2.1.2 pine pollen
Songke Song genus Masson pine (PinusmassonianaLamb.), Chinese Pine (P.tabulaeformisCarr.) Or belong to several species of plants
Male germ cells.
2.2 broken pollen
Processed to be broken pollen, pollen cell wall.
3 Technical requirements
3.1 Requirements for raw materials
Raw materials shall comply with the relevant food standards and regulations.
3.2 Sensory requirements
Sensory requirements shall comply with the requirements in Table 1.
Table 1 Sensory requirements
project
Claim
Bee pollen pine pollen
Testing method
Color with a pale yellow color product due
Taste, smell
Bee pollen has the proper taste and odor-free
Odor, no different olfactory
Pine pollen should have the taste and odor-free
Odor, no different olfactory
status
Flat, round or irregular powder pellet (particles
Tablets), no insects, no mildew, no normal vision
Visible foreign matter
Fine powder, light weight, good fluidity, easy to fly,
There are hand twist smooth sense, not visible outside normal vision
To foreign body
Proper amount of sample is placed in clean white
Color wheel (porcelain or similar container),
Observe the color under natural light, and
Status, check for foreign objects, smell it
Smell, after rinsing with warm water products thereof
taste
3.3 Physical and Chemical Indicators
Physical and chemical indicators should be consistent with the provisions of Table 2.
Table 2. Physical and chemical indicators
project
index
Bee pollen pine pollen
Testing method
Water/(g/100g) ≤ 10.0 8.0 GB 5009.3 drying under reduced pressure
Ash/(g/100g) ≤ 5.0 4.5 GB 5009.4
Protein/(g/100g) ≥ 15.0 9.0 GB 5009.5 Kjeldahl method
Bee pollen single species of pollen /% ≥ 85 - APPENDIX A
Acidity (expressed in pH) ≥ 4.4 -
20g samples were taken in a conical flask, 100mL of purified
Water, Shakers, Erlenmeyer flask immersed in a water bath set to 75 deg.] C 1.5h.
It cooled to room temperature, the qualitative filter paper, 0.45 m filtered.
The filtrate, and then press the acidity or pH meter operating instructions into
Line measurements, and record the pH, accurate to 0.02, pH results
Expressed as average of two measurements
3.4 pollutant limit
Pollutant limits should comply with the provisions of GB 2762.
3.5 Microbiological
Microbiological ready to eat pre-packaged product shall be in accordance with Table 3.
Table 3 Microbiological
project
And a limited sampling program
ncm M
Testing method
Cfu/(CFU/g) 5 2 103 104 GB 4789.2
Coliforms/(MPN/g) 5 2 4.3 46 GB 4789.3
Mold/(CFU/g) ≤ 2 × 102 GB 4789.15
Sampling and processing a sample performed in GB 4789.1.
4 Other
A single species of bee pollen, bee pollen should identify varieties on the label.
Appendix A
Pollen for determination of a single species of bee pollen
A.1 Instruments
A.1.1 microscope. 10 to 100 times.
A.1.2 centrifuge. speed 10000r/min.
A.2 Reagents
A.2.1 sulfuric acid and glacial acetic acid (volume ratio of 1.9).
A.2.2 glycerol (AR).
A.3 sample handling
Bee pollen 1g ~ 2g take place 15mL graduated centrifuge tube, adding sulfuric acid and glacial acetic acid (1.9) a mixture of 2mL, pollen immersion
grain. Mashed with a glass rod pollen, boiling water bath for 5min. Cooling, distilled water volume to 10mL. Centrifugation, the supernatant was discarded, washed repeatedly by centrifugation
Step 3 times. Glycerol was added dropwise number precipitate mix. 1 with a glass rod dipped drop coated onto a glass slide, covered with a coverslip.
A.4 count
Take already processed slides were observed under the microscope at 10x or 40x objective. The smear found pollen grains too dense resulting in overlap
When Count of, should be re-smears, the number of pollen grains per field preferably in the range of 30 ~ 100. Select 5 visual field of view and
All counts pollen grains in the field, and with different authentication control map pollen species. The number of particles of a variety of pollen 5 Perspective view with 5
All wild than the total number of pollen grains of pollen rate is the single species of bee pollen. Each test should be carried out parallel tests.
A.5 bee pollen (pollen single species of pollen hetero) Identification
A.5.1 rapeseed (Brassicacampestris) pollen
Canola flower pink yellow. It has a substantially prolate pollen grains, according to morphological characteristics into campestris, Brassica juncea. Circular equatorial view
Or oval, three-lobed polar view of a sheet, three grooves significant germination. Pole face diameter of about 42μm, the diameter of the equatorial plane of about 40μm. Figure A.1.
a) canola flower powder form equatorial view (× 400) b) polar view canola flower powder form (× 400)
Figure A.1 canola flower powder particle morphology under a microscope
A.5.2 sesame (Sesamumindicum) pollen
Sesame pollen white or brown. Chengbian spherical pollen grains (like a flat pumpkin), a small spherical. Equatorial view is wide oval, very
11 to 12 lateral view lobed circular. Glyph tumor-like surface, as viewed from the front minus mesh, from about 35μm × 40μm. Having 10 ~ 13
Germination groove, wider gap. Pole face diameter of about 45μm, the diameter of the equatorial plane of about 65μm. See Figure A.2.
a) sesame pollen morphology equatorial view (× 400) b) polar view sesame pollen morphology (× 400)
Figure A.2 sesame morphology of pollen grains under a microscope
A.5.3 buckwheat (Fagopyrumesculentum) pollen
Buckwheat pollen dark yellow. Pollen grains elongated spherical surface with fine mesh Glyph equatorial view is oval, three-lobed polar view circle
shape. Polar viewing three clearly visible germination groove. Pole face diameter of about 44μm, the diameter of the equatorial plane 31μm. See Figure A.3.
a) buckwheat pollen morphology equatorial view (× 100) B) polar view buckwheat pollen morphology (× 100)
Figure A.3 buckwheat pollen grains morphology under a microscope
A.5.4 sunflower (Helianthusannuus) pollen
Sunflower pollen as an orange. Pollen grains are spherical, oval equatorial view polar view of 3-lobed circular diameter of about 35μm.
The outer wall of spikes, barbed length of 3μm ~ 5μm. Colporate surface 3, 5μm ~ 10μm spacing. Figure A.4.
a) sunflower pollen morphology equatorial view (× 400) b) polar view sunflower pollen morphology (× 400)
Figure A.4 sunflower pollen grains morphology under a microscope
A.5.5 corn (Zea mays) pollen
As a pale yellow corn pollen. Pollen particles are approximately spherical, diameter of about 80μm. Smooth outer wall, there is a circular hole germination. Figure A.5.
a) maize pollen morphology equatorial view (× 400) b) maize pollen morphology polar view (× 400)
Figure A.5 corn pollen particle morphology under a microscope
A.5.6 vetch (Astragalussinicus) pollen
Chinese milk vetch pollen is orange. Pollen grains elongated spherical, oblong equatorial view polar view blunt triangular sheet-like or three-lobed. pole
Surface diameter of about 30μm, the diameter of the equatorial plane of about 20μm. Glyph surface has fine mesh, there tricolporate. Figure A.6.
a) milk vetch pollen morphology equatorial view (× 400) b) polar view milk vetch pollen morphology (× 400)
Figure A.6 milk vetch morphology of pollen grains under a microscope
A.5.7 sorghum (Sorghumbicolor) pollen
Pollen is light yellow. Pollen particles are spherical, circular equatorial view polar view blunt triangular sheet-like or three-lobed. Pole face diameter of about
30μm, equatorial diameter of about 30μm. Surface of the fine mesh, there tricolporate. Figure A.7.
a) sorghum pollen morphology equatorial view (× 400) b) polar view sorghum pollen morphology (× 400)
FIG A.7 sorghum pollen grains morphology under a microscope
A.6 calculation results
The detection results by the formula (A.1) is calculated.
X =
b × 100%
(A.1)
Where.
X --- single species of bee pollen rate,%;
A --- The number of particles bee pollen species, in units of grains;
Sample B --- Total bee pollen grains, grain units.
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