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GB 31610.6-2023 English PDF

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GB 31610.6-2023: (National food safety standards - Inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products)
Status: Valid
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GB 31610.6-2023English139 Add to Cart 3 days [Need to translate] (National food safety standards - Inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products) Valid GB 31610.6-2023

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Basic data

Standard ID GB 31610.6-2023 (GB31610.6-2023)
Description (Translated English) (National food safety standards Inspection of Anisakis in animal aquatic products and their products)
Sector / Industry National Standard
Classification of Chinese Standard X09
Word Count Estimation 11,174
Date of Issue 2023-09-06
Date of Implementation 2024-03-06
Issuing agency(ies) National Health Commission of the People's Republic of China, State Administration for Market Regulation
Summary This standard specifies the morphological and PCR detection methods of Anisakidae larvae in animal aquatic products and their products. This standard is applicable to the inspection of Anisakis larvae in animal aquatic products and their products.

GB 31610.6-2023: (National food safety standards - Inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products)


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Standards of People's Republic of China National food safety standards Animal-based aquatic products and their products Testing for sparganosis of the tapeworm Mansoni Published on 2023-09-06 Implemented on 2024-03-06 National Health Commission of the People's Republic of China Released by the State Administration for Market Regulation National food safety standards Animal-based aquatic products and their products Testing for sparganosis of the tapeworm Mansoni

1 Scope

This standard specifies the morphology and morphology of Spirometramansoni larvae in animal aquatic products and their products. PCR test method. This standard is applicable to the inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products.

2 Principles

Sparganoid larvae of the tapeworm Mansoni in animal aquatic products and their products mainly live in the muscles of frogs and other aquatic products. Obtained from anatomical separation Based on the morphological structure of the sparganoides, the species of the sparganoides can be preliminarily determined; by amplifying the cells of the mitochondrial DNA of the sparganoid tapeworm Mansoni The cytochrome oxidase (coxI) gene fragment was sequenced to identify the sparganoid larvae of the tapeworm S. mansoni.

3 Instruments and equipment

3.1 Biological microscope. 40×~100×. 3.2 Stereo microscope. 7.5×~150×. 3.3 PCR amplification instrument. 3.4 Gel imaging system. 3.5 Electrophoresis apparatus. 3.6 High-speed centrifuge. speed ≥12000r/min. 3.7 Micropipette. 0.2μL~2.5μL, 1μL~10μL, 10μL~100μL, 100μL~1000μL.

4 Reagents and materials

4.1 Reagents 4.1.1 1mol/LTris-HCl solution (pH8.0). 4.1.2 0.5mol/LEDTA solution (pH8.0). 4.1.3 10% SDS solution. 4.1.4 5mol/LNaCl solution. 4.1.5 20mg/mL proteinase K. 4.1.6 Phenol/chloroform/isoamyl alcohol (25.24.1). 4.1.7 Chloroform. 4.1.8 5U/μL thermostable DNA polymerase. 4.1.9 10×PCR buffer. 4.1.10 25mmol/LMgCl2.

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