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GB 31610.5-2023 English PDF

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GB 31610.5-2023: (National food safety standards Inspection of Paragonimus in animal aquatic products and their products)
Status: Valid
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GB 31610.5-2023English159 Add to Cart 3 days [Need to translate] (National food safety standards Inspection of Paragonimus in animal aquatic products and their products) Valid GB 31610.5-2023

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Basic data

Standard ID GB 31610.5-2023 (GB31610.5-2023)
Description (Translated English) (National Food Safety Standards Inspection of Gnatostome nematodes in animal aquatic products and their products)
Sector / Industry National Standard
Classification of Chinese Standard X09
Word Count Estimation 8,825
Date of Issue 2023-09-06
Date of Implementation 2024-03-06
Issuing agency(ies) National Health Commission of the People's Republic of China, State Administration for Market Regulation
Summary This standard specifies the morphological and PCR detection methods of Gnathostoma larvae in animal aquatic products and their products. This standard is applicable to the inspection of Gnatostoma larvae in animal aquatic products and their products.

GB 31610.5-2023: (National food safety standards Inspection of Paragonimus in animal aquatic products and their products)


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Standards of People's Republic of China National food safety standards Inspection of Paragonimus in animal aquatic products and their products Published on 2023-09-06 Implemented on 2024-03-06 National Health Commission of the People's Republic of China Released by the State Administration for Market Regulation National food safety standards Inspection of Paragonimus in animal aquatic products and their products

1 Scope

This standard specifies the morphology and PCR testing of Paragonimus metacercariae in animal aquatic products and their products. method. This standard applies to the inspection of Paragonimus metacercariae in animal aquatic products and their products.

2 Principles

Metacercariae of Paragonimus in animal aquatic products and their products mainly live in the muscle tissue of crustaceans such as crabs, crickets, and macrobrachials. Mash the sediment to separate or use pepsin to digest the animal host tissue after mashing to obtain metacercariae. Based on the morphological characteristics of Paragonimus metacercariae, preliminary Determine the type of metacercariae; by amplifying and sequencing the second internal transcribed spacer (ITS2) gene fragment of Paragonimus ribosomal DNA, the combined Identification of metacercariae of Geniminus.

3 Instruments and equipment

3.1 Biological microscope. 100×~400×. 3.2 Stereo microscope. 7.5×~150×. 3.3 PCR amplification instrument. 3.4 Gel imaging system. 3.5 Electrophoresis apparatus. 3.6 Constant temperature incubator. 37℃±1℃. 3.7 High-speed centrifuge. speed ≥12000r/min. 3.8 Mesh screen. aperture 2mm (10 mesh); aperture 0.425mm (40 mesh). 3.9 Micropipette. 0.2μL~2.5μL, 1μL~10μL, 10μL~100μL, 100μL~1000μL. 3.10 Conical measuring cup. 1000mL. 3.11 Glass beads. 425μm~600μm. 3.12 Ceramic mortar (diameter 16cm) and grinding rod. 3.13 Cyclotron oscillator.

4 Reagents and materials

4.1 Reagents 4.1.1 Hydrochloric acid. 36%~38% HCl solution. 4.1.2 Physiological saline. 0.85% NaCl solution. 4.1.3 1mol/LTris-HCl solution (pH8.0). 4.1.4 0.5mol/LEDTA solution (pH8.0).

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