HOME   Cart(0)   Quotation   About-Us Policy PDFs Standard-List
www.ChineseStandard.net Database: 189759 (19 Oct 2025)

GB 23200.98-2016 English PDF

US$219.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB 23200.98-2016: Food safety national standard -- Determination of Eleven Organophosphorus Pesticide Residues in Royal Jelly by Gas Chromatography
Status: Valid
Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB 23200.98-2016English219 Add to Cart 3 days [Need to translate] Food safety national standard -- Determination of Eleven Organophosphorus Pesticide Residues in Royal Jelly by Gas Chromatography Valid GB 23200.98-2016

PDF similar to GB 23200.98-2016


Standard similar to GB 23200.98-2016

GB/T 38211   GB/T 18418   GB/T 18419   GB 23200.102   GB 23200.103   GB 23200.101   

Basic data

Standard ID GB 23200.98-2016 (GB23200.98-2016)
Description (Translated English) Food safety national standard -- Determination of Eleven Organophosphorus Pesticide Residues in Royal Jelly by Gas Chromatography
Sector / Industry National Standard
Classification of Chinese Standard G25
Word Count Estimation 11,115
Date of Issue 2016-12-18
Date of Implementation 2017-06-18
Older Standard (superseded by this standard) SN/T 2577-2010
Regulation (derived from) State Health Commission, Ministry of Agriculture, Food and Drug Administration Notice No. 16 of 2016
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration

GB 23200.98-2016: Food safety national standard -- Determination of Eleven Organophosphorus Pesticide Residues in Royal Jelly by Gas Chromatography


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Food safety national standard - Determination of Eleven Organophosphorus Pesticide Residues in Royal Jelly by Gas Chromatography National Standards of People's Republic of China GB Replace SN/T 2577-2010 National standards for food safety Determination of Eleven Organophosphorus Pesticide Residues in Royal Jelly Gas chromatography National food safety standards- Determination of eleven organophosphorus pesticides residues in royal jelly Gas chromatography 2016-12-18 Release.2017-06-18 Implementation National Health and Family Planning Commission of the People 's Republic of China Issued by the Ministry of Agriculture of the People 's Republic of China State Administration of Food and Drug Administration

Foreword

This standard replaces SN/T 2577-2010 "Import and export royal jelly in 11 kinds of organophosphorus pesticide residues determination of gas chromatography." Compared with SN/T 2577-2010, the main changes are as follows. - Standard text format is modified to national standard text format for food safety; - standard name "import and export royal jelly" to "royal jelly"; - increase the "other food reference implementation" in the standard range. This standard replaced the previous version of the standard release. -SN/T 2577-2010. National standards for food safety Determination of Eleven Organophosphorus Pesticide Residues in Royal Jelly by Gas Chromatography

1 Scope

This standard specifies the preparation of 11 kinds of organophosphorus pesticide residues in royal jelly and the determination of sample preparation and gas chromatography. This standard applies to royal jelly in the dichlorvos, methamidophos, broken phosphorus, phorate, dimethoate, methyl parathion, malathion, parathion, Quetiapine, triazophos, phosphorus poison phosphorus pesticide residues detection, other food can refer to the implementation.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB 2763 National Standard for Food Safety - Maximum Residue Limit of Pesticides in Foodstuffs GB/T 6682 Analytical laboratory water specifications and test methods

3 principle

The organophosphorus pesticide was extracted from the sample with acetonitrile. The extract was purified by gel column chromatography using a gas chromatograph equipped with a flame photometric detector Quantitative and external standard method.

4 reagents and materials

Unless otherwise specified, all reagents are of analytical grade and water is in accordance with the primary water specified in GB/T 6682. 4.1 Reagents 4.1.1 Ethyl acetate (C4H8O2). Chromatographic purity. 4.1.2 acetonitrile (CH3CN). pure chromatography. 4.1.3 Cyclohexane (C6H12). Chromatographic Purification. 4.1.4 Acetone (C3H6O). Chromatographic pure. 4.1.5 Sodium chloride (NaCl) 4.1.6 anhydrous sodium sulfate (Na2SO4). 650 ℃ burning 4h, in the dryer to cool to room temperature, stored in a sealed bottle in reserve. 4.2 solution preparation 4.2.1 GPC eluent, ethyl acetate cyclohexane (1 1, volume ratio). take 100 mL of ethyl acetate, add 100 mL of cyclohexane, shake Evenly used. The 4.3 standards 4.3.1 dichlorvos, methamidophos, methotrexate, phorate, dimethoate, methyl parathion, malathion, parathion, quinophos, triazophos, Flies poison phosphorus standard. purity ≥ 99%, see Appendix A Table A.1. 4.4 standard solution preparation 4.4.1 11 kinds of organic phosphorus standard stock solution. Weigh the appropriate standard, respectively, with acetone dissolved to 100 mL, the solution concentration 100 g/mL, 1 ℃ ~ 4 ℃ refrigerator. Valid for 3 months. 4.4.2 11 kinds of organic phosphorus mixed standard working solution. before use as necessary with acetone diluted to the appropriate concentration, as a mixed standard working fluid. 1 ℃ ~ 4 ℃ refrigerator to save. Valid for 3 months. 4.4.3 Standard working solution solution concentration. 0.008 g/mL, 0.01 g/mL, 0.05 g/mL, 0.1 g/mL, 0.5 g/mL, Stored in the refrigerator at 4 ℃. 4.5 Materials 4.5.1 Gel Purification Product Line (GPC). 400 mm x 15 mm (inner diameter); Filler. Bio-Beads, S-X3, 38 m-75 m Before the need to use the standard solution to do the first leaching curve). 4.5.2 anhydrous sodium sulfate column. 80 mm × 40 mm (diameter) cylindrical funnel, the bottom pad 5 mm absorbent cotton, then install 50 mm anhydrous sodium sulfate. 4.5.3 N-propyl ethylenediamine bonded silica gel (PSA)

5 instruments and equipment

5.1 Gas Chromatograph with Flame Photometric Detector (Phosphor Filter 525 nm). 5.2 Analysis of balance. 0.0001 g and 0.01 g. 5.3 Rotary Evaporator 5.4 vortex mixer. 5.5 Centrifuge ≥6 000 r/min.

6 Preparation and storage of samples

Take 500 g representative royal jelly samples, the sampling site according to GB 2763 Appendix A implementation, thawed at room temperature, and other samples after all melted Stir the sample, the samples are divided into two copies, respectively, into the vial, sealed, and marked. One as a test sample and one at -18 ° C save. During the operation of the sample preparation, the contamination of the sample or the change in the content of the residue should be prevented.

7 Analysis steps

7.1 Extraction Weigh 5 g sample (accurate to 0.01 g) in a 50 mL plastic centrifuge tube, add 15 mL of water, mix for 5 min, add B Nitrile to 50 mL on a vortex mixer at 2 000 r/min, mix for 1 min, centrifuge at 6 000 r/min for 5 min, remove the supernatant The solution was placed in another 50 mL plastic centrifuge tube and 4 g of sodium chloride was added to the vortex mixer at 2 000 r/min for 1 min, Centrifuge at 6 000 r/min for 5 min. The upper layer of acetonitrile was passed through an anhydrous sodium sulphate column, collected in a concentrated flask, and then 5 mL of water and 20 ML of acetonitrile to the residue, repeat the above operation, combined acetonitrile extract, 40 ℃ in the following water bath concentrated to near dry, to be purified. 7.2 Purification Residue with a solvent volume to 5 mL, mix, take 1.0 mL supernatant GPC column, flow rate 1 mL/min, with solvent elution, before abandoning 15 mL of eluent, collected 15 mL to 30 mL of the eluent, below 40 ° C in the water bath under reduced pressure to near dry, add 8 mL of acetone dissolved, In 40 ℃ below the water bath with a gentle nitrogen flow to dry, accurately add 1.0 mL of acetone, mix, add 0.05-0.1gPSA mix, over 0.45 m filter for gas chromatography. 7.3 Determination 7.3.1 Chromatographic reference conditions A) Column. quartz capillary column, DB-1701, 30 m x 0.53 mm (inner diameter) x 1.0 μm (film thickness), or equivalent; B) Carrier gas. nitrogen (purity greater than 99.999%); constant pressure mode. 89.635 kPa (13 psi); tail gas flow rate. 30 mL/min; Hydrogen flow rate. 75 mL/min, air flow rate. 90 mL/min; C) Column temperature. initial temperature 120 ° C for 1 min, rise to 230 ° C at 10 ° C/min, rise to 270 ° C at 20 ° C/min Min; D) Inlet temperature. 250 ° C; E) Detector temperature. 250 ° C; F) Injection mode. pulse splitless injection, pulse pressure 172.375 kPa (25 psi), pulse time 0.75 min; G) Injection volume. 4 μL; H) Opening time. 0.75 min; 7.3.2 Chromatographic determination According to the content of organophosphorus pesticides in the sample, the standard working solution with similar peak area is selected. Standard working solution and sample solution The response value of organophosphorus should be within the linear range of the instrument. Standard working solution and sample volume and other volume interspersed injection determination. In the above chromatographic conditions Dichlorvos, methamidophos, methicillin, chlorophosphate, phorate, dimethoate, methyl parathion, malathion, parathion, quinophos, triazophos, And the reference retention time was about 2.9 min, 3.9 min, 7.3 min, 7.9 min, 10 min, 11.0 min, 11.3 min, 11.9 Min, 12.2 min, 14.5 min, 19.2 min. The chromatogram of the standard is shown in Figure B.1 in Appendix B. 7.4 blank test In addition to no sample, according to the above steps.

8 results are calculated and expressed

Use the chromatographic data processor or according to formula (1) to calculate the residual content of various organic phosphorus in the sample, the calculation results need to deduct the blank value. MA VcA IS I   (1) Where. Xi - Residue of organophosphorus pesticide in milligrams per kilogram (mg/kg); The peak area of organic phosphorus pesticide in sample solution; Ais - the standard working fluid in the organic phosphorus pesticide peak area; Ci - The concentration of organophosphorus pesticide in standard working fluid, in micrograms per milliliter (g/mL); V - the final volume of the sample solution in milliliters (mL); M - the final sample quality of the sample, in grams (g). Note. The result of the calculation shall be deducted from the blank value. The result of the measurement shall be expressed as the arithmetic mean of the parallel measurement, and two valid digits shall be retained.

9 precision

9.1 The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with Appendix D requirements. 9.2 The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with Appendix E requirements. 10% limit and recovery rate 10.1 Quantitation limits The limit of 11 kinds of organophosphorus residues was 0.01 mg/kg. 10.2 Recovery rate The experimental data of the recoveries (within the range of different additive concentrations) are given in Appendix C.

Appendix A

(Normative appendix) Table A.1 Eleven organophosphate standards information Standard substance English name CAS NO. Molecular formula Molecular weight Dichlorvos Dichlirvos 52-68-6 C4H7O4CL2P 220.98 Methamidophos 10265-92-6 C2H8NO2PS 141.0 Phenanthroline Ethoprophos 13194-48-4 C8H19O2PS2 242.33 Phorate Phorate 298-02-2 C7H17O2PS3 260.38 Dimethoate 60-51-5 C5H12NO3PS2 229.12 Parathion-methyl 298-00-0 C8H10NO5PS 263.20 Malathion 121-75-5 C10H19O6PS2 330.36 Parathion 56-38-2 C10H14NO5PS 291.26 Quetiapine Quinalphos 13593-03-8 C12H15N2O3PS 298.29 Triazophos 24017-47-8 C12H16N3O3PS 313.31 Flies Phosphorus Coumaphos 56-72-4 C14H16CLO5PS 362.78

Appendix B

(Informative) Standard chromatogram 1 - dichlorvos (2.9 min) 2 - methamidophos (3.9 min) 3 - off line phosphorus (7.3 min) 4 - Phorate (7.9 min) 5 - dimethoate (10 min) 6 - methyl parathion (11.0 min) 7 - malathion (11.3 min) 8 - parathion (11.9 min) 9 - quetiophosphate (12.2 min) 10 - triazophos (14.5 min) 11 - fly poison phosphorus (19.2 min) Figure B.1 Gas chromatograms of 11 organic phosphorus mixed standard solutions (0.05 g/mL)

Appendix C

(Informative) Sample concentration and recovery of the experimental data Table C.1 Experimental data on the concentration and recovery of the sample Compound Concentration/(mg/kg) Recovery Range /% Precision Range /% Dichlorvos 0.01 82.6  97.2 5.3 0.05 92.4  101.4 3.5 0.1 88.598.6 4.4 Methamidophos 0.01 70.6  80.7 6.2 0.05 72.8  97.6 10.6 0.1 75.1 99.7 11.7 Off line phosphorus 0.01 106.5 110.0 1.5 0.05 93.6  100.0 2.5 0.1 90.9  99.9 4.0 Phorate 0.01 93.8 99.3 2.4 0.05 85.8  99.8 5.6 0.1 81.8  97.0 7.0 Dimethoate 0.01 97.5 110.0 4.1 0.05 71.2  78.6 3.4 0.1 80.6  89.7 3.8 Methyl parathion 0.01 96.0  109.5 4.7 0.05 95.0  105.5 4.1 0.1 91.9  106.6 6.5 Malathion 0.01 96.1 110.0 4.9 0.05 82.4  91.6 4.0 0.1 80.4  97.1 7.4 Parathion 0.01 97.0  109.1 3.9 0.05 84.8  102.2 6.9 0.1 80.7  90.8 5.0 Quetiapine 0.01 95.3  106.1 3.6 0.05 73.2  95.8 10.2 0.1 70.8  78.6 4.2 Triazophos 0.01 78.2 98.5 7.9 0.05 89.0  106.2 5.9 0.1 82.6  100.2 8.8 Flies phosphorus 0.01 100.1  109.8 3.3 0.05 86.6  106.2 7.0 0.1 88.0 110.0 10.0

Appendix D

(Normative appendix) Laboratory repeatability requirements Table D.1 Laboratory repeatability requirements Measured component content Mg/kg Precision 0.001 36 > 0.01 > 1 14

Appendix E

(Normative appendix) Inter-laboratory reproducibility requirements Table E.1 Inter-laboratory reproducibility requirements Measured component content Mg/kg Precision 0.001 54 > 0.01 > 1 19

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB 23200.98-2016_English be delivered?

Answer: Upon your order, we will start to translate GB 23200.98-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB 23200.98-2016_English with my colleagues?

Answer: Yes. The purchased PDF of GB 23200.98-2016_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.