HOME   Cart(0)   Quotation   About-Us Policy PDFs Standard-List
www.ChineseStandard.net Database: 189759 (19 Oct 2025)

GB 23200.40-2016 English PDF

US$219.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB 23200.40-2016: Food safety national standard -- Determination of organophosphorus and organochlorine pesticide residues in cola drinks -- Gas chromatographic method
Status: Valid
Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB 23200.40-2016English219 Add to Cart 3 days [Need to translate] Food safety national standard -- Determination of organophosphorus and organochlorine pesticide residues in cola drinks -- Gas chromatographic method Valid GB 23200.40-2016

PDF similar to GB 23200.40-2016


Standard similar to GB 23200.40-2016

GB/T 38211   GB/T 18418   GB/T 18419   GB 23200.33   GB 23200.34   GB 23200.32   

Basic data

Standard ID GB 23200.40-2016 (GB23200.40-2016)
Description (Translated English) Food safety national standard -- Determination of organophosphorus and organochlorine pesticide residues in cola drinks -- Gas chromatographic method
Sector / Industry National Standard
Classification of Chinese Standard G25
Word Count Estimation 11,197
Date of Issue 2016-12-18
Date of Implementation 2017-06-18
Older Standard (superseded by this standard) SN/T 1984-2007
Regulation (derived from) State Health Commission, Ministry of Agriculture, Food and Drug Administration Notice No. 16 of 2016
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration

GB 23200.40-2016: Food safety national standard -- Determination of organophosphorus and organochlorine pesticide residues in cola drinks -- Gas chromatographic method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Food safety national standard - Determination of organophosphorus and organochlorine pesticide residues in cola drinks - Gas chromatographic method National Standards of People's Republic of China GB Instead of SN/T 1984-2007 National standards for food safety Coke drinks in organic phosphorus, organic chlorine Determination of pesticide residues Gas chromatography National food safety standards- Determination of organophosphorus and organochlorine residues In cola drink Gas chromatography 2016-12-18 Release.2017-06-18 Implementation National Health and Family Planning Commission of the People 's Republic of China Issued by the Ministry of Agriculture of the People 's Republic of China State Administration of Food and Drug Administration

Foreword

This standard replaces SN/T 1984-2007 "Method for the determination of organophosphorus and organochlorine pesticide residues in import and export cola beverages Gas chromatography law". Compared with SN/T 1984-2007, the main changes are as follows. - Standard text format is modified to national standard text format for food safety; - Standard name "import and export cola drinks" to "cola drinks". - increase the "other food reference implementation" in the standard range. This standard replaced the previous version of the standard release. -SN/T 1984-2007. National standards for food safety Determination of organophosphorus and organochlorine pesticide residues in cola drinks - Gas chromatographic method

1 Scope

This standard specifies the determination of 11 organophosphorus and organochlorine pesticide residues in cola beverages by gas chromatography. This standard applies to cola drinks in dichlorvos, chlorpyrifos, malathion, parathion, heptachlor, hexachlorobenzene, hexachloride and its isomeric (11.66, β-six six six, γ-six six six, δ-six six six), pentachloronitrobenzene and other 11 kinds of organic phosphorus, organochlorine pesticide residues Of the detection, other food can refer to the implementation.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB 2763 National Standard for Food Safety - Maximum Residue Limit of Pesticides in Foodstuffs GB/T 6682 Analytical laboratory water specifications and test methods

3 principle

The organic phosphorus residue in the sample was extracted with ethyl acetate, concentrated by rotary evaporation, purified by solid phase extraction column, and analyzed by gas chromatography flame photometric Determination of the external standard method. The organochlorine residue in the sample was extracted with ethyl acetate, concentrated by rotary evaporation, sulfonated and purified by gas chromatography electron capture detector, External standard method.

4 reagents and materials

Unless otherwise specified, all reagents are of analytical grade and water is in accordance with the primary water specified in GB/T 6682. 4.1 Reagents 4.1.1 n-hexane (C6H14). chromatographic purity. 4.1.2 Methanol (CH3OH). Chromatographic Purification. 4.1.3 ethyl acetate (C4H8O2). excellent grade pure. 4.1.4 anhydrous sodium sulfate (Na2SO4). 650 ℃ burning 4 h, placed in the dryer in reserve. 4.1.5 Sodium chloride (NaCl). 4.1.6 Sodium hydroxide (NaOH). 4.1.7 concentrated sulfuric acid (H2SO4). excellent grade pure. 4.2 solution preparation 4.2.1 Methanol - Aqueous solution (5 95, V/V). Take 5 mL of methanol and mix with 95 mL of water. 4.2.2 Methanol - Aqueous solution (10 90, V/V). Take 10 mL of methanol and mix with 90 mL of water. 4.2.3 Sodium hydroxide solution (1 mol/L). 20 g of sodium hydroxide, dissolved in water and set to 500 mL. 4.3 standards 4.3.1 Pesticide Reference Material. See Appendix A. 4.4 standard solution preparation 4.4.1 Organophosphorus pesticide standard solution 4.4.1.1 Organophosphorus pesticide standard stock solution. accurately weighed the amount of dichlorvos, chlorpyrifos, malathion, parathion, respectively with C The ketone is dissolved and fixed in a brown volumetric flask at a concentration of 1 000 mg/L and the stock is stored below -18 ° C. 4.4.1.2 organic phosphorus mixed standard intermediate solution. accurate absorption of appropriate dichlorvos, chlorpyrifos, malathion, parathion standard stock solution In a brown volumetric flask, the concentration of various organophosphorus pesticide standard solutions is 10 mg/L, and the intermediate solution is at -18 ° C Save the following. 4.4.1.3 organic phosphorus mixed standard working solution. with ethyl acetate mixed standard intermediate solution as needed dilution, prepared for the four kinds of Machine phosphorus pesticide mixed standard working solution, mixed standard working solution in 0 ℃ ~ 4 ℃ preservation. 4.4.2 Organochlorine pesticide standard solution 4.4.2.1 mixed solution of organochlorine pesticide standard solution. remove the appropriate volume of heptachlor, hexachlorobenzene, 666 mixed standard solution, pentachlor Nitrobenzene standard solution in a brown volumetric flask, with n-hexane volume to the scale, a variety of organochlorine pesticide standard solution concentration of 10 mg/L, The intermediate solution is stored below -18 ° C. 4.4.2.2 organic chlorine mixed standard working solution. with n-hexane mixed standard intermediate solution as needed dilution, formulated as organic chlorine farmers Drug mixed standard working solution, mixed standard working solution stored at 0 ℃ ~ 4 ℃. 4.5 Materials 4.5.1 HLB solid phase extraction cartridge. 60 mg, 3 mL, or equivalent.

5 instruments and equipment

5.1 Gas Chromatograph, Electron Capture Detector (ECD) and Flame Photometric Detector (FPD) Phosphorescent filter at a wavelength of 525 nm. 5.2 Analysis of balance. 0.01 g and 0.0001 g. 5.3 Centrifuge. 5 000 r/min. 5.4 blowing nitrogen enrichment instrument. 5.5 Solid phase extraction device. 5.6 Rotary Evaporator. 5.7 separatory funnel. 500mL. 5.8 Milling glass round bottom flask. 500 mL. 5.9 glass sand core funnel. 5.10 polypropylene with a plug centrifuge tube. 15mL. 5.11 Vortex Mixer.

6 Analysis steps

6.1 Extraction Take the cola sample in a beaker, place it for 60 min, and stir the exhaust with a glass rod. Accurately weigh 150 g (accurate to 0.01 g) cola Sample, add 1 mol/L sodium hydroxide solution to adjust the pH of the solution to about 7. Transfer the neutralized cola sample to 500 mL In the separatory funnel, add 15 g of sodium chloride and 100 mL of ethyl acetate, shake vigorously for 2 min and leave for 10 min. Layer of organic phase, pre-filled anhydrous sodium sulfate column (in the glass sand core funnel filled with about 15 g anhydrous sodium sulfate, and 20 mL B Ethyl acetate rinse) was collected in a 500 mL round bottom flask. In the separatory funnel,.200 mL of ethyl acetate was added in two portions, 100 mL each time, Repeat the above extraction steps, the combined extract in 40 ℃ water bath rotation to 3 mL ~ 4 mL, transferred to 15 mL centrifuge tube, with 9 ML n-hexane was washed three times in a round-bottomed flask, and the combined washings were mixed in a 15 mL centrifuge tube and blown nitrogen to near dry at 40 ° C. 6.2 purification 6.2.1 Determination of organophosphorus pesticide sample solution HLB solid phase extraction column before use, respectively, with ethyl acetate 3 mL leaching once, 3 mL of methanol and 3 mL of water pretreated twice to keep the column Body moist. The extract of 6.1 was dissolved in 5 mL of a 10% aqueous methanol solution and passed through an HLB solid phase extraction column at 1 drop/sec. And the tube was rinsed with 10 mL of 5% aqueous methanol and the HLB column was discarded. The rinsing solution from the HLB column was discarded and the vacuum was drained 10 min. Eluting with 9 mL of ethyl acetate. The eluate was collected in a 15 mL centrifuge tube, 3 g of anhydrous sodium sulfate was added, shaken for 3 min, 5000 r/min under the conditions of centrifugation 2 min, the solution transferred to another centrifuge tube, in the 40 ℃ water bath blowing nitrogen concentrated to near dry, with acetic acid B The ester was set to 1.0 mL and the gas chromatographic FPD was determined. 6.2.2 Determination of organochlorine pesticides Sample solution purification The extract of 6.1 was dissolved in 1 mL of n-hexane, 0.5 mL of concentrated sulfuric acid was added, the mixture was gently shaken and centrifuged at 5000 r/min for 5 min After taking the upper organic phase into the gas chromatography ECD determination, such as the purification effect is not sufficient, can be added again concentrated sulfuric acid purification. 6.3 Determination 6.3.1 Gas chromatographic electron capture detection instrument reference conditions A) Column. DB-5 quartz capillary column. 30 m x 0.32 mm (inner diameter), film thickness 0.25 μm, or equivalent. B) Column temperature. 80 ° C (1 min) 30 ° C/min.180 ° C 3 ° C/min 205 ° C (4 min) 2 ° C/min 210 ° C (1 min), postrun. 280 ° C (1 min); C) Inlet temperature. 250 ° C. D) Detector temperature. 300 ° C. E) Carrier gas. nitrogen, purity 99.999%, constant pressure 0.135Mpa. F) Injection volume. 1 μL. G) Injection method. split injection, split ratio of 12. 1. 6.3.2 Gas chromatographic flame photometric detection instrument reference conditions A) Column. DB-1701 quartz capillary column. 30 m x 0.32 mm (inner diameter), film thickness 0.25 μm, or equivalent. B) Column temperature. 90 ° C (1 min) 30 ° C/min. 2.0 ° C (8 min) 10 ° C/min 240 ° C, postrun. 280 ° C (5 min). C) Inlet temperature. 250 ° C. D) Detector temperature. 250 ° C. E) Carrier gas. nitrogen, purity 99.999%, constant pressure 0.071Mpa. F) Injection volume. 1 μL. G) Injection method. no split into the sample, 1.0 min after the opening of the diversion valve, purge flow 50 mL/min. 6.3.3 Determination and confirmation of chromatography The measured solution was subjected to gas chromatographic electron capture detection and gas chromatography flame photometric detection, according to the sample solution of organic phosphorus or organic chlorine Residual content of the situation, selected peak area similar to the standard working solution. Standard working solutions and residual reactions of organophosphorus or organochlorine residues in the sample solution The values shall be within the linear range of the instrument's detection. For the mixed standard working solution and sample solution, such as volume fractionation method, the external standard method. The retention times of organophosphorus and organochlorine pesticides are shown in Appendix A under 6.3.1 and 6.3.2 chromatographic conditions. 10 μg/L organic phosphorus and organic chlorine mixed standard The chromatogram of the quasi-working solution is shown in Appendix B, Figure B.1, Figure B.2, respectively. 6.4 blank experiment In addition to the sample, according to the above determination steps.

7 Results calculation and presentation

The contents of the organophosphorus and organochlorine pesticide compounds in the sample were calculated according to the following formula (1). MΑs VCsA   .. (1) In formula (1). X-sample organic phosphorus, organochlorine pesticide compound content in milligrams per kilogram, mg/kg; A sample of organic phosphorus, organochlorine pesticide compound peak area; AS-standard working solution of organic phosphorus, organochlorine pesticide compound peak area; Cs-standard working solution of organic phosphorus, organochlorine pesticide compound concentration in milligrams per liter, mg/L; M - the amount of sample weighed, in grams, g; V-sample liquid final volume, in milliliters, mL. Note. The result of the calculation shall be deducted from the blank value. The result of the measurement shall be expressed as the arithmetic mean of the parallel measurement, and two valid digits shall be retained.

8 precision

8.1 The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with the Record the requirements of D. 8.2 The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with the Record the requirements of E.

9 Quantitation limit and recovery rate

9.1 Limit of limits The limit of organic phosphorus and organochlorine pesticides is 0.0001 mg/kg. 9.2 Recovery When the levels were 0.0001 mg/kg, 0.0002 mg/kg, 0.0005 mg/kg, the addition of organophosphorus, organochlorine pesticide compounds See Appendix C. for the yield.

Appendix A

(Informative) Organic phosphorus, organochlorine pesticide basic information and retention time Table A.1 Basic information and retention times for organophosphorus pesticides Serial number Pesticide Chinese name Pesticide English name CAS number Molecular formula Molecular retention time Min Dichlorvos 62-73-7 C4H7Cl2O4P 220.98 4.750 2 chlorpyrifos 2921-88-2 C9H11Cl3NO3PS 350.59 11.246 3 Malathion 121-75-5 C10H9O6PS2 330.36 12.028 4 Parathion 56-38-2 C10H14NO5PS 291.26 13.250 Table A.2 Basic information and retention times of organochlorine pesticides Serial number Pesticide Chinese name Pesticide English name CAS number Molecular formula Molecular weight keep time Min 1 α-hexahydrate α-HCB 319-84-6 C6H6Cl6 290.83 9.657

2 hexachlorobenzene

Hexachlorobenz Ene 118-74-1 C6Cl6 284.78 9.924 3 β-hexamethylene β-HCB 319-85-7 C6H6Cl6 290.83 10.378 4 γ-hexahydrate γ-HCB 58-89-9 C6H6Cl6 290.83 10.591 5 Pentachloronitrobenzene Quintozene 82-68-8 C6Cl5NO2 295.34 10.749 6 δ-hexahydrate δ-HCB 319-86-8 C6H6Cl6 290.83 11.284 7 Hexachlor Heptachlor 76-44-8 C10H5Cl7 373.32 13.072

Appendix B

(Informative) Organic phosphorus, organic chlorine pesticide reference substance chromatogram Figure B.1 10 μg/L organic phosphorus pesticide standard solution chromatogram 1. dichlorvos; 2. chlorpyrifos; 3. malathion; 4. parathion. Figure B.2.10 μg/L Organic Chlorine Pesticide Standard Solution Chromatogram 1. а-six six six; 2. hexachlorobenzene; 3. β-666; 4. pentachloronitrobenzene; 5. γ-666; 6.

Appendix C

(Informative) The recovery rate of organic phosphorus and organochlorine pesticides in cola Table C.1 Recovery of organic phosphorus and organochlorine pesticides in cola No. Compound name Recovery rate range,% 1 dichlorvos 72.0% ~ 105.0 2 chlorpyrifos 75.8% ~ 98.0 3 malathion 64.0% ~ 88.0 4 parathion 75.0% ~ 92.0 5 α - 6 6 81.5% ~ 99.0 6 hexachlorobenzene 85.0% ~ 106.0 7 ~ 6 6 6 73.0% ~ 98.0 8 ~ 6 6 6 84.0% ~ 104.6 9 Pentachloronitrobenzene 81.5% ~ 98.0 10 δ-six six six 76.0% ~ 97.0 11 Heptachlor 81.0% ~ 109.0

Appendix D

(Normative appendix) Laboratory repeatability requirements Table D.1 Laboratory repeatability requirements Measured component content Mg/kg Precision 0.001 36 > 0.01 > 1 14

Appendix E

(Normative appendix) Inter-laboratory reproducibility requirements Table E.1 Inter-laboratory reproducibility requirements Measured component content Mg/kg Precision 0.001 54 > 0.01 > 1 19

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB 23200.40-2016_English be delivered?

Answer: Upon your order, we will start to translate GB 23200.40-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB 23200.40-2016_English with my colleagues?

Answer: Yes. The purchased PDF of GB 23200.40-2016_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.