|
US$239.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB 15955-2011: [GB/T 15955-2011] Gibberellic acid technical material
Status: Valid GB 15955: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB 15955-2011 | English | 239 |
Add to Cart
|
3 days [Need to translate]
|
[GB/T 15955-2011] Gibberellic acid technical material
| Valid |
GB 15955-2011
|
| GB 15955-1995 | English | 239 |
Add to Cart
|
2 days [Need to translate]
|
Gibberellins technical
| Obsolete |
GB 15955-1995
|
PDF similar to GB 15955-2011
Basic data | Standard ID | GB 15955-2011 (GB15955-2011) | | Description (Translated English) | [GB/T 15955-2011] Gibberellic acid technical material | | Sector / Industry | National Standard | | Classification of Chinese Standard | G25 | | Classification of International Standard | 65.10.30 | | Word Count Estimation | 9,960 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-04-15 | | Older Standard (superseded by this standard) | GB 15955-1995 | | Quoted Standard | GB/T 613-2007; GB/T 1604; GB/T 1605-2001; GB 3796 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Chinese standard specifies the original drug gibberellic acid requirements, test methods and marking, labeling, packaging, storage and acceptance period. This standard applies to gibberellic acid and its impurities generated in the production of gibberellic acid composition of the original drug. | GB 15955-2011: [GB/T 15955-2011] Gibberellic acid technical material---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Gibberellic acid technical material
ICS 65.10.30
G25
National Standards of People's Republic of China
Replacing GB 15955-1995
Gibberellic acid original drug
Issued on. 2011-12-30
2012-04-15 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The standard Chapter 3, Chapter 5 is mandatory, the rest are recommended.
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Since the implementation of this standard, instead of GB 15955-1995 "GA original drug."
The main differences between standard and GB 15955-1995 "GA original drug" as follows.
--- "GA" to "gibberellic acid";
--- Canceled and classification;
--- Gibberellic acid to the mass fraction ≥90.0%;
--- Loss on drying by the "≤1.0%" to "≤0.5%";
--- Specific rotation to specific rotation ability αm (20 ℃, D) ≥ 80 (°) · m2/kg.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
The standard proposed by China Petroleum and Chemical Industry Association.
This standard by the National Standardization Technical Committee on Pesticides (SAC/TC133) centralized.
This standard was drafted. the Ministry of Agriculture, Zhejiang Qianjiang Biochemical Co., Ltd., Shanghai with Swiss biotechnology Limited
Secretary, Jiangxi Ruifeng Sheng Chemical Co., Ltd.
The main drafters of this standard. Wang Xiaoli, Li Youshun, Chentie Chun, Zhao Yonghui, Yu Rong, Zhu Jianxin, Chenming Lei, Lu love sun.
Gibberellic acid original drug
1 Scope
This standard specifies requirements for gibberellic acid original drug, test methods and marking, labeling, packaging, storage and acceptance period.
This standard applies to the impurities generated by the production of gibberellic acid, and gibberellic acid composition of the original drug.
Note. Other name, structural formula and basic physicochemical parameters of gibberellic acid in Appendix A.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 613-2007 General method for determination of chemical reagents specific rotation ability (specific optical rotation)
GB/T 1604 Goods pesticide regulations for acceptance
GB/T 1605-2001 Sampling Method commercial pesticides
GB 3796 pesticide packaging General
3 Requirements
3.1 Composition and appearance
This product should gibberellic acid and impurities generated in the production of the composition, it should be white to light yellow crystalline powder, free from visible foreign matter and filling
Modifier.
3.2 Project and indicators
Gibberellic acid original drug should be consistent with the requirements in Table 1.
Table 1 gibberellic acid original drug control project indicators
Item Index
Gibberellic acid mass fraction /% ≥ 90.0
Loss on drying /% ≤ 0.5
The specific rotation ability αm (20 ℃, D)/[(°) · m2/kg] ≥ 80
Test Method 4
4.1 Sampling
According to GB/T 1605-2001 "on the original drug product sampling" approach. Random sampling method is determined by the package, the final amount of sample lot
At 100g.
4.2 Identification Test
The identification test can be carried out simultaneously with the determination of the mass fraction of gibberellic acid. Under the same chromatographic operating conditions, the main sample solution chromatogram
Retention time and peak standard solution of gibberellic acid chromatographic peak retention time, the relative difference should be less than 1.5%.
4.3 Determination of the mass fraction of gibberellic acid
4.3.1 Method summary
Sample dissolved in mobile phase, methanol - phosphoric acid as the mobile phase, use as filler to C18 stainless steel column and UV detector, for
Gibberellic acid in the sample by reverse phase HPLC separation and determination of external standard.
4.3.2 Reagents and solutions
Phosphoric acid. AR;
Methanol. HPLC grade;
Water. The new secondary steam distilled water;
Phosphoric acid. [Psi] (water. phosphoric acid) = 2000.1;
Gibberellic acid standard sample. a known mass fraction w≥98.0%.
4.3.3 Instruments
High performance liquid chromatography. a variable wavelength UV detector and quantitative injection valve;
Chromatographic data processor or chromatography workstation;
Column. 250mm × 4.6mm (id) stainless steel column, built ZORBAX80ÅEXTENDC18 filler (or equivalent
Other column effect);
Micro injector. 100μL;
Filter. filter pore size of about 0.45μm;
Ultrasonic cleaner.
4.3.4 HPLC operating conditions
Mobile phase. [Psi] (methanol. aqueous phosphoric acid solution) = 35.65;
Flow rate. 1.0mL/min;
Column temperature. room temperature (temperature change should not exceed 2 ℃);
Detection wavelength. 210nm;
Injection volume. 10μL.
Retention time. gibberellic acid about 8.2min.
HPLC operating conditions described above, the Department of typical operating parameters, according to the characteristics of different instruments, given operating parameters to make appropriate adjustments to
To obtain the best results. Typical gibberellic acid original drug HPLC is shown in Figure 1.
1 --- gibberellic acid.
Figure 1 HPLC gibberellic acid original drug
4.3.5 measuring step
4.3.5.1 preparation of standard solution
Weigh gibberellic acid standard about 0.05g (accurate to 0.0002g), placed in 100mL flask, add 35mL of methanol to dissolve, then
Phosphoric acid aqueous solution was diluted to the mark.
4.3.5.2 preparation of the sample solution
Weigh a sample containing about 0.05g of gibberellic acid (accurate to 0.0002g), placed in 100mL flask, add 35mL of methanol to dissolve,
Phosphoric acid aqueous solution and then diluted to the mark. Filtered through a 0.45μm pore size filters.
4.3.5.3 Determination
Under these operating conditions, after the instrument is stable, continuous injection of several doses of the standard solution until the two adjacent needles gibberellic acid peak area relative change
After less than 1.5%, according to the solution, sample solution, sample solution, standard order of standard solution was measured.
4.3.6 Calculation
The two needle sample solution and measured before and after the two doses of the standard sample solution of gibberellic acid peak area were averaged. Sample gibberellic acid
The mass fraction according to equation (1).
w1 = A2
· M1 · w
A1 · m2
(1)
Where.
w1 --- sample gibberellic acid content, expressed as a percentage;
A2 --- sample solution, gibberellic acid, the average peak area;
m1 --- gibberellic acid standard mass values in grams (g);
Mass fraction w --- standard samples gibberellic acid, expressed in%;
A1 --- standard solution, gibberellic acid, the average peak area;
Numerical m2 --- sample mass, in grams (g).
4.3.7 allowable difference
The difference between two parallel determination results shall not be greater than 1.5%, the arithmetic mean value as a measurement result.
4.4 Determination of loss on drying
4.4.1 Instruments and Appliances
Oven. 105 ℃ ± 2 ℃;
Weighing bottle. diameter 50mm, height 20mm;
Dryer.
4.4.2 measuring step
The weighing bottle placed in 105 ℃ ± 2 ℃ oven baking 1h, and then into the dryer to cool to room temperature, weighed (accurate to 0.0002g).
Repeat the above steps until constant weight weighing bottle. In weighing bottle was weighed into a 1g sample (paved weighed accurate to 0.0002g). Will be referred
Flask placed in an oven, bake uncovered 1h, the lid is removed and placed in the dryer to cool to room temperature, weighed (accurate to 0.0002g).
4.4.3 Calculation
Loss on drying of the sample according to equation (2).
w2 = m1-m2m
(2)
Where.
w2 --- Loss on drying, in%;
Mass m1 --- weighing bottle and sample before drying, in grams (g);
Mass m2 --- weighing bottle and sample after drying, in grams (g);
M --- the quality of the sample, in grams (g).
4.5 Determination of specific rotation ability
Performed according to GB/T 613-2007 requirements. Wherein the solution to be tested is gibberellic acid ethanol solution of the original drug at a concentration of 0.1g/mL. As solution
After the sample is turbid solution was found, after application of rapid quantitative determination of filter paper. Sample specific rotation ability αm (20 ℃, D) [(°) · m2/kg],
According to equation (3) Calculated.
αm (20 ℃, D) = 100
· Α · V
m 1-0.01w () 2 l
(3)
Where.
α --- measured rotation angle in degrees (°);
The V --- volume of the sample dilution in milliliters (mL of);
M --- the quality of the sample, in grams (g);
W2 --- Loss on drying of the sample, expressed in%;
--- optical tube length L, the unit decimeter (dm).
4.6 product testing and acceptance
Shall comply with GB/T 1604's. Treatment of extreme values, using the rounding value comparison method.
5 marking, labeling, packaging, storage, security, acceptance of
5.1 mark, label
Gibberellic acid original drug marking, labeling and packaging should comply with the provisions of GB 3796.
5.2 Packaging
Gibberellic acid original drug is appropriate, clean, dry metal pail lined with plastic, cardboard, aluminum foil or plastic bucket bag, net weight of each piece is generally
25kg. According to user requirements or the order agreement, the use of other forms of packaging, it should comply with the provisions of GB 3796.
5.3 Storage
Gibberellic acid original drug package should be stored in ventilated, cool, dry coffers, fire, moisture, and can not be in the open air with toxic substances, flammable
Explosive materials and basic materials stacked together. Not with food, seeds, feed mixed release.
Storage and transportation to prevent moisture and sun, and rain to avoid collision, away from fire.
5.4 Security
This product is a low toxicity plant growth regulator. The FDA should wear protective gloves, masks, wearing clean protective clothing. Not with the use of basic
Mixed goods, can not be mixed with ammonia, calcium polysulfide, soda ash and the like. Used with the distribution, once dubbed the aqueous solution can not go far. Immediately after use with
Wash water. In case of poisoning, should go to hospital for treatment.
5.5 Acceptance of
Acceptance of gibberellic acid original drug is a month. From the date of delivery, product quality inspection completed within a month, the indicators should meet
Combined standards.
Appendix A
(Informative)
Other names gibberellic acid, structural formula and basic physicochemical parameters
This product is an active ingredient gibberellic acid is another name, structural formula and basic physicochemical parameters are as follows.
ISO common name. Gibberelicacid
CIPAC Numeric Code. 307
CAS Registry Number. 77-06-5
Chemical name. (1α, 2β, 4aα, 4bβ, 10β) -2,4a, 7- trihydroxy-1-methyl-8-methyl-3-ene-1,10-gibberellic acid -1 , 4a-
Lactone
Structure.
Empirical formula. C19H22O6
Molecular Weight. 346.4
Biological activity. Plant Growth Regulator
Melting point. 223 ℃ ~ 225 ℃ (decomposition)
Solubility. water 5g/L (room temperature). Soluble in methanol, ethanol, acetone, alkaline aqueous solution, slightly soluble in diethyl ether and ethyl acetate, insoluble
Chloroform. Its potassium, sodium, ammonium salts soluble in water (potassium salt 50g/L)
Stability. At room temperature in a dry state is stable, slow degradation in aqueous or aqueous alcohol solution in, DT50 (20 ℃) about
14d (pH3 ~ 4), 14d (pH7), alkali metal will be converted into less biologically active compounds, the case of thermal decomposition.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 15955-2011_English be delivered?Answer: Upon your order, we will start to translate GB 15955-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB 15955-2011_English with my colleagues?Answer: Yes. The purchased PDF of GB 15955-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. Question 5: Should I purchase the latest version GB 15955-2011?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB 15955-2011 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.
|