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SN/T 2643-2010: Cultivar identification and purity detection of Thai Jasmine rice
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2643-2010 | English | 259 |
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Cultivar identification and purity detection of Thai Jasmine rice
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SN/T 2643-2010
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Basic data | Standard ID | SN/T 2643-2010 (SN/T2643-2010) | | Description (Translated English) | Cultivar identification and purity detection of Thai Jasmine rice | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X11 | | Classification of International Standard | 67.060 | | Word Count Estimation | 10,191 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 6682; SN/T 0798; SN/T 0800.1; SN/T 1193 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Thai jasmine rice variety identification and purity testing DNA test, sensory testing method and poached Inspection Act. This standard applies to Thai jasmine rice in the rice varieties KDMLl05 and RDl5 legal identity and purity test. | SN/T 2643-2010: Cultivar identification and purity detection of Thai Jasmine rice---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Cultivar identification and purity detection of Thai Jasmine rice
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Thai jasmine rice variety identification and purity test method
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some content of this document may involve patents, publication of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Xiamen People's Republic of China Exit Inspection and Quarantine, Continental test (Thailand
Country) Limited.
The main drafters of this standard. Wu Yajun, Zhang Zhenmin, Chen Ying, Wang Bin, Xu Baoliang, Liu Jianguo, Huang Wensheng, Fei Yuan.
Thai jasmine rice variety identification and purity test method
1 Scope
This standard specifies the Thai jasmine rice variety identification and purity testing DNA test, sensory test and boiling test.
This standard applies to the statutory Thai jasmine rice in rice varieties KDML105 and RD15 identity and purity test.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 0798 import and export grain, feed testing terminology
SN/T 0800.1 and export grain, feed inspection sampling and sample preparation methods
SN/T 1193 genetic testing laboratory technical requirements
3 Terms and Definitions
SN/T 0798 defined by the following terms and definitions apply to this document.
3.1
Thai jasmine rice ThaiJasminerice; ThaiHomMalirice; ThaiFrangrantrice
By the Bureau of Agriculture of Thailand, the Ministry of Agriculture and Cooperatives non-waxy aromatic rice varieties registered KaoDokMali105 or RD15 rice by
Brown rice or polished rice milling obtained.
3.2
Varietal purity vatietalpurity
The quality of this variety of the percentage of the sample.
3.3
Polymerase chain reaction polymerasechainreaction; PCR
Located used to amplify DNA (deoxyribonucleosideaacid, DNA) sequence between the two known methods. template
High temperature DNA denaturation into single strands, DNA polymerase and at a suitable temperature, two mutually complementary oligonucleotide primers fragment that is divided
Do annealing occurs with some complementary sequence on both strands of the template DNA, followed by catalytic DNA polymerase in four dNTP as substrate
Material, primer annealing can be extended, and so forth denaturation, annealing and DNA synthesis of this circulation, it is located between two known sequences
DNA fragment was amplified geometrically, by 25 to 30 cycles of amplification, the amplification factor is at about 106.
3.4
Random amplified polymorphic analysis randomamplificationpolymorphicDNA; RAPD
Using a series of random arrangement of oligonucleotides (typically ten-mers) as primers and genomic DNA PCR amplification were studied
increase. After amplification product was purified by polyacrylamide or agarose gel electrophoresis, EB staining or by autoradiography to detect the amplification product
Polymorphic DNA fragments amplified polymorphic DNA fragments which reflects the corresponding genomic DNA polymorphism region.
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