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SN/T 2602-2010: Detection and identification of Agrobacterium tume faciens (Smith et townsend 1907) Conn 1942
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2602-2010 | English | 259 |
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Detection and identification of Agrobacterium tume faciens (Smith et townsend 1907) Conn 1942
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SN/T 2602-2010
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Basic data | Standard ID | SN/T 2602-2010 (SN/T2602-2010) | | Description (Translated English) | Detection and identification of Agrobacterium tume faciens (Smith et townsend 1907) Conn 1942 | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,118 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Quoted Standard | GB/T 4789.28-2003 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry and exit of plant quarantine in Agrobacterium tumefaciens rod seedlings detection and identification methods. This standard applies to entry and exit of plants and their products on the detection of Agrobacterium tumefaciens identification. | SN/T 2602-2010: Detection and identification of Agrobacterium tume faciens (Smith et townsend 1907) Conn 1942
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Agrobacterium tume faciens (Smith et townsend 1907) Conn 1942
People's Republic of China Entry-Exit Inspection and Quarantine Standards
The method of detection and identification of Agrobacterium tumefaciens
(Smithettownsend1907) Conn1942
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shandong CIQ, People's Republic of China Tianjin Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Li Yan, Wang Premiership, Wu Xinghai, Shao Xiuling, Ganqin Hua, Liu Peng.
The method of detection and identification of Agrobacterium tumefaciens
1 Scope
This standard specifies the export plant quarantine in Agrobacterium tumefaciens detection and identification methods.
This standard applies to the entry and exit of Agrobacterium tumefaciens on the detection and identification of plants and their products.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
GB/T 4789.28-2003 Microbiological examination of food hygiene staining, media and reagents
Principle 3
Agrobacterium tumefaciens Agrobacteriumtumefaciens (Smithettownsend1907) Conn1942, genus Prokaryotae
(Procaryoces), thin-walled bacteria door (Gracilicutes), Rhizobium Branch (Rhizobiaceae), Agrobacterium (Agrobacterium). Disease
Morphological characteristics of bacteria, biological characteristics, biochemical characteristics, pathogenicity and specificity of PCR reactions is based on this standard quarantine identified.
4 apparatus and appliances
Biological microscope, stereo microscope, clean benches, electronic scales, light incubator, temperature oscillation incubator, autoclave,
Petri dishes (diameter. 7.5cm), constant temperature water bath (30 ℃ ~ 95 ℃), high-speed refrigerated centrifuge (18000r/min or more), BIOLOG Kam
Given instrument, PCR instrument, electrophoresis apparatus (electrophoresis, horizontal electrophoresis tank), gel imaging analyzer, micro adjustable pipette (2μL, 10μL, 20μL,
100μL, 200μL, 1000μL).
5 Reagents
Peptone, hydrogen peroxide (H2O2), mannitol, yeast extract, magnesium sulphate (MgSO4 · 7H2O), agar, glycerol, mercuric chloride, hydrochloric acid, hypochlorite
Sodium ethylene diamine tetra-acetic acid (EDTA), Tris (Tris), yellow sodium dodecyl (SDS), liquid nitrogen, chloroform, isoamyl
Alcohol, isopropyl alcohol, agarose (for electrophoresis), ethidium bromide, TaqDNA polymerase, dipotassium hydrogen phosphate (K2HPO4 on), potassium dihydrogen phosphate
(KH2PO4), disodium hydrogen phosphate (Na2HPO4 · 12H2O), ammonium dihydrogen phosphate (NH4H2PO4), calcium chloride (CaCl2), sodium chloride
(NaCI), potassium chloride (KCl), 10 × PCR buffer, 10 × dNTP solution, distilled water, beef extract, hydrochloric acid, dimethyl-p-phenylenediamine,
95% ethanol.
6 Detection Methods
6.1 isolated pathogens
6.1.1 Separation plant pathogenic bacteria
Selection of plants should be isolated or suspected galls galls, try to use white young galls. First galls or galls suspected washed,
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