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SN/T 2596-2010: Detection and identification of Pseudomonas syringae pv. tomato(Okabe) Young et al. 1978
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2596-2010 | English | 239 |
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Detection and identification of Pseudomonas syringae pv. tomato(Okabe) Young et al. 1978
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SN/T 2596-2010
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Basic data | Standard ID | SN/T 2596-2010 (SN/T2596-2010) | | Description (Translated English) | Detection and identification of Pseudomonas syringae pv. tomato(Okabe) Young et al. 1978 | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 9,994 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Quoted Standard | GB/T 6682; SN/T 1538.1; SN/T 1809 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry and exit of tomato plant quarantine in quarantine bacterial leaf spot identification methods. This standard applies to the entry and exit of tomato and pepper seeds and their products in the tomato leaf spot fungus thin Zhuo quarantine and identification. | SN/T 2596-2010: Detection and identification of Pseudomonas syringae pv. tomato(Okabe) Young et al. 1978
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Detection and identification of Pseudomonas syringae pv.tomato (Okabe) Young et al.1978
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Tomato bacterial leaf spot quarantine and identification of bacteria
pv.tomato (Okabe) Youngetal.1978
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Tianjin People's Republic of China Exit Inspection and Quarantine, the People's Republic of China
Zhejiang Exit Inspection and Quarantine and the State.
The main drafters of this standard. Liu Peng, Wang Jincheng, Zhao Wenjun, Wu Zhiyi, Dingcui Zhen, Liuyue Ting, Guo Jingze.
Tomato bacterial leaf spot quarantine and identification of bacteria
1 Scope
This standard specifies the identification of entry and exit quarantine phytosanitary tomato bacterial leaf spot fungus.
This standard applies to entry and exit quarantine and identification of tomato and pepper seeds and tomato products of bacterial leaf spot.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1809 export plant quarantine rules for seed
Principle 3
Tomato bacterial leaf spot genus Prokaryotae (Procaryotes), thin-walled bacteria door (Gracilicutes), Pseudomonas Branch (Pseudo-
monaceae), Pseudomonas (Pseudomonas), the biological characteristics of the pathogen, serological properties and biological properties of the molecule is formulated
Mainly based on the standard (see Appendix A).
4 apparatus and appliances
Biological microscope, clean benches, autoclave, microplate reader, incubator, centrifuges, PCR instrument, electrophoresis equipment, Gel Imaging
Systems and other routine laboratory equipment and apparatus.
5 Reagents
Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. It shall comply with GB 6682 test water in a water specifications.
Culture, bacterial leaf spot of tomato reagents used in Appendix B, Appendix C and Appendix D.
Quarantine and identification of 6
6.1 Field Survey and Plant Inspection
When collecting a field investigation of suspicious symptoms or fruit plants back to the lab, rinse with water, dried with sterile scalpel in
Disease key junction take a small piece of diseased tissue suspended in a small amount of sterile water using a sterile glass rod extrusion, and then dipped into the bacterial suspension inoculation loop, in
KB medium plate streaking, thermostat 28 ℃ culture 48h, according to colony characteristics of tomato bacterial leaf spot of suspicious colonies were selected
Further characterization can also be used as a stem or fruit onset by ELISA test.
6.2 Seed Treatment
Random 30g (≥10000 tablets) tomato seeds as a sample (less than 30g tomato seed samples are all extraction, pumping concrete
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