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Import and export of food of animal origin 19 go methyltestosterone residues determination method of gas chromatography mass spectrometry
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SN/T 1826-2006
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Basic data | Standard ID | SN/T 1826-2006 (SN/T1826-2006) | | Description (Translated English) | Import and export of food of animal origin 19 go methyltestosterone residues determination method of gas chromatography mass spectrometry | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X22;C53 | | Classification of International Standard | 67.120.01 | | Word Count Estimation | 17,175 | | Date of Issue | 2006-11-10 | | Date of Implementation | 2007-05-16 | | Regulation (derived from) | Chinese industry standards for record 2007 of 3 (total of 87) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of food of animal origin in the liver, kidney and muscle, 19 nortestosterone residues method. This standard applies to food of animal origin in the liver, kidney and muscle, 19 nortestosterone residues. | SN/T 1826-2006: Import and export of food of animal origin 19 go methyltestosterone residues determination method of gas chromatography mass spectrometry
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Import and export of food of animal origin 19 go methyltestosterone residues determination method of gas chromatography mass spectrometry
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
19-nortestosterone residues in imported and exported animal foods
Determination of quantity by gas chromatography-mass spectrometry
GC-MS
Released on.2006-11-10
2007-05-16 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted by the Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Han Li, Li Bo, Guo Dehua, Yang Jingxian, Yang Huiqin, Wang Min.
This standard is the first industry standard for entry-exit inspection and quarantine.
19-nortestosterone residues in imported and exported animal foods
Determination of quantity by gas chromatography-mass spectrometry
1 Scope
This standard specifies the method for determination of 19-nortestosterone residues in liver, kidney and muscle in foods for import and export.
This standard applies to the determination of 19-nortestosterone residues in liver, kidney and muscle in animal-derived foods.
2 Determination method
2.1 Method summary
The target compound in animal tissues is enzymatically hydrolyzed in buffer, extracted with methanol, degreased in n-hexane, and the extract is purified by C18 cartridge.
After the derivatization reaction, it was determined by gas chromatography-mass spectrometry and quantified by external standard method.
2.2 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade and the water was distilled water.
2.2.1 β-glucuronidase. 100 U/mL.
2.2.2 Glacial acetic acid.
2.2.3 Sodium acetate.
2.2.4 Methanol.
2.2.5 n-hexane. excellent grade pure.
2.2.6 Ether.
2.2.7 Sodium hydroxide.
2.2.8 isooctane. excellent grade pure.
2.2.9 Heptafluorobutyric anhydride.
2.2.10 1mol/L sodium hydroxide solution. dissolve 40g sodium hydroxide in 1L water.
2.2.11 Acetate buffer solution (0.2mol/L, pH=5.2±0.2). 16.4g of sodium acetate dissolved in 900mL of water, adjusted with glacial acetic acid
The pH was adjusted to 5.2 ± 0.2, and the volume was adjusted to 1 L with water.
2.2.12 19-nortestosterone standard, purity ≥ 99%.
2.2.13 19-nortestosterone standard solution. accurately weigh the appropriate amount of 19-nortestosterone standard, and prepare a concentration of 100 μg/mL with methanol.
Standard stock solution. The standard solution is diluted with methanol to a standard working solution of the appropriate concentration as needed.
2.3 Instruments and equipment
2.3.1 Gas Chromatography-Mass Spectrometry (GC-MS).
2.3.2 High speed homogenizer.
2.3.3 Centrifuge. 4000r/min.
2.3.4 Vortex mixer.
2.3.5 Rotate the evaporator.
2.3.6 C18 solid phase extraction cartridge. 500mg, activated with 5mL methanol and 5mL water in sequence, and set aside.
2.3.7 Nitrogen blowing instrument.
2.3.8 2 mL Derivatized vial with screw cap (with Teflon-lined gasket).
2.4 Determination steps
2.4.1 Preparation of samples
A representative sample of about 1 kg was taken from all the animal tissue samples taken, fully ground, mixed, and divided into two parts, respectively
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