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GB/T 37226-2018: Criterion of forensic science human fluorescence STR multiplex amplification reagent
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 37226-2018 | English | 279 |
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Criterion of forensic science human fluorescence STR multiplex amplification reagent
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GB/T 37226-2018
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Basic data | Standard ID | GB/T 37226-2018 (GB/T37226-2018) | | Description (Translated English) | Criterion of forensic science human fluorescence STR multiplex amplification reagent | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A92 | | Classification of International Standard | 13.310 | | Word Count Estimation | 14,180 | | Date of Issue | 2018-12-28 | | Date of Implementation | 2018-12-28 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 37226-2018: Criterion of forensic science human fluorescence STR multiplex amplification reagent
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Criterion of forensic science human fluorescence STR multiplex amplification reagent
ICS 13.310
A92
National Standards of People's Republic of China
Forensic Science Human Fluorescent Labeling STR Complex Amplification
Basic requirements for testing reagent quality
Published on.2018-12-28
2018-12-28 implementation
State market supervision and administration
China National Standardization Administration issued
Content
Foreword III
1 Scope 1
2 Terms and Definitions 1
3 Technical requirements 2
4 Detection method 4
5 Identification, packaging and transportation, storage 7
Appendix A (informative) 9947A positive control typing results 8
Reference 9
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This standard is under the jurisdiction of the National Criminal Technology Standardization Technical Committee (SAC/TC179).
The main drafting unit of this standard. the physical evidence appraisal center of the Ministry of Public Security.
Participated in the drafting of this standard. Guangzhou Municipal Public Security Bureau, Beijing Public Security Bureau, Liaoning Provincial Public Security Department, Shanghai Public Security Bureau, Ministry of Public Security Criminal Technology
Surveillance Bureau, Tianjin Public Security Bureau.
Drafters of this standard. Ge Baichuan, Ye Jian, Liu Chao, Liu Yacheng, Jiang Xianhua, Zhao Xingchun, Zhou Huaigu, Zhang Qingxia, Wang Haiou, Jin Jinzhi, Bai Xue.
Forensic Science Human Fluorescent Labeling STR Complex Amplification
Basic requirements for testing reagent quality
1 Scope
This standard specifies the technical requirements and tests for forensic human fluorescent labeling STR complex amplification detection reagents (hereinafter referred to as "reagents").
Method, identification, packaging, transportation and storage.
This standard applies to the production, use and quality inspection of human fluorescent labeled STR complex amplification detection reagents used in forensic science.
The use of "reagents" in other fields can be referred to the adoption of this standard.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Short tandem repeat shorttandemrepeat; STR
A class of DNA sequences of length polymorphisms present in human genomic DNA, the core sequence of which is generally composed of 2 to 6 bases.
The base composition differs between individuals due to the difference in the number of repetitions of the core sequence.
2.2
Locus locus
The position of a gene on a chromosome or a segment of genomic DNA.
2.3
Polymerase chain reaction polymerasechainreaction; PCR
An enzymatic amplification process of a specific DNA fragment in vitro.
2.4
Human fluorescent labeled STR complex amplification detection reagent humanfluorescentSTRmultiplexamplificationreagent
Synchronization of multiple STR loci in human DNA samples using polymerase chain reaction (PCR) and fluorescent labeling
(2.2) A series of reagents for performing STR genotyping in human samples by performing complex amplification and obtaining STR locus typing.
2.5
Conventional reagent generalreagent
Using the extracted biological sample DNA as a template, a type of forensic human fluorescent label for STR typing was obtained by PCR (2.3) reaction.
Record STR complex amplification detection reagent (2.4).
2.6
Direct amplification reagent DirectPCRreagent
Direct use of biological samples containing blood (plaques) and saliva (plaques) in the PCR (2.3) reaction system to release the sample in the reaction system
DNA, a class of forensic human fluorescent labeling STR complex amplification detection reagent (2.4) that obtained STR typing.
Note. The direct amplification reagent should be marked with the words “direct amplification” on the reagent packaging.
2.7
Typing accuracy veracity
Reagent test positive DNA control sample typing results and the specified classification results.
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