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GB/T 28984-2012: Detection and identification of banana bract mosaic virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 28984-2012 | English | 279 |
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Detection and identification of banana bract mosaic virus
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GB/T 28984-2012
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Basic data | Standard ID | GB/T 28984-2012 (GB/T28984-2012) | | Description (Translated English) | Detection and identification of banana bract mosaic virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 12,147 | | Regulation (derived from) | National Standards Bulletin No. 41 of 2012 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the banana bract mosaic virus serology and molecular biological characteristics of the detection method. This standard applies to plants such as banana and plantain plantlets host plant material is banana bract mosaic virus detecti | GB/T 28984-2012: Detection and identification of banana bract mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of banana bract mosaic virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Banana bract mosaic virus quarantine and identification methods
Issued on. 2012-12-31
2013-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China, Ningbo Entry-Exit Inspection and Quarantine Shanghai People's Republic of China
Bureau, Chinese Academy of Inspection and Quarantine, People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters of this standard. Wen Weigang, Guo Lixin, Yang Cuiyun, Chen Xianfeng, Wing Lei, Zhang Yongjiang, Zhang Mingzhe, Xu Ying, Cuijun Xia, Zhang Huili.
Banana bract mosaic virus quarantine and identification methods
1 Scope
This standard specifies the method for detection of banana bract mosaic virus serological and molecular biological characteristics.
This standard applies to bananas and other Musa tissue culture and plant host plant material detected in banana bract mosaic virus, also applies to the aphid
Insects and other media to detect the virus.
2 banana bract mosaic virus Basic Information
Chinese name. banana bract mosaic virus
Name. bananabractmosaicvirus
Abbreviation. BBrMV
Potato virus Y belongs to the genus (Potyvirus), additional information, see Appendix A. banana bract mosaic virus
3 PRINCIPLE OF THE METHOD
Based on the use of antigen-antibody reaction of the double-antibody sandwich enzyme-linked immunosorbent assay (Doubleantibodysandwichenzyme-linked
immunosorbentassay, DAS-ELISA), reverse transcription polymerase chain reaction in vitro DNA synthesis technology (Reverse
transcriptionpolymerasechainreaction, RT-PCR), real-time fluorescence reverse transcription-polymerase chain reaction (real-time
fluorescentRT-PCR) detection and identification.
4 equipment, appliances and reagents
4.1 Equipment
Conventional PCR, real-time PCR instrument, clean benches, electronic balance (the amount of sense. 0.001g), electrophoresis, electrophoresis tank, gel imaging
System, high-speed refrigerated centrifuge, ultra-low temperature freezer (-80 ℃), autoclaves, small-scale food processing machines, microwave ovens, microplate reader.
4.2 Appliances
Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and the corresponding RNase-free tip, no
RNase centrifuge tubes, PCR tubes and mortar and the like.
4.3 Reagents
Reagents and buffers see Appendices B and C.
5 Detection
5.1 Sample Preparation
Take 0.5g ~ 10g deformity, discoloration and other symptoms leaves or other plant tissues, in a mortar or small food processor thoroughly ground,
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