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Detection and identification of strawberry latent ringspot virus
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GB/T 28976-2012
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Basic data | Standard ID | GB/T 28976-2012 (GB/T28976-2012) | | Description (Translated English) | Detection and identification of strawberry latent ringspot virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 16,180 | | Quoted Standard | SN/T 1840; SN/T 2122 | | Regulation (derived from) | National Standards Bulletin No. 41 of 2012 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the strawberry latent ringspot virus quarantine identification. This standard applies to plant material, including vegetative propagation material, seeds and seedlings of strawberry latent ringspot virus quarantine identification. | GB/T 28976-2012: Detection and identification of strawberry latent ringspot virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of strawberry latent ringspot virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Strawberry latent ringspot virus quarantine and identification methods
Issued on. 2012-12-31
2013-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Ningbo Entry-Exit Inspection and Quarantine of People's Republic of China
Bureau, People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Yang Cuiyun at Chui, Wen Weigang, Zheng Jianzhong, India Liping, Li Bin.
Strawberry latent ringspot virus quarantine and identification methods
1 Scope
This standard specifies the Strawberry latent ringspot virus, quarantine and identification methods.
This standard applies to plant material, including vegetative propagation material, seeds and seedlings in quarantine and identification of strawberry latent ringspot virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1840 plant virus immune electron microscopy method
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 Strawberry latent ringspot virus basic information
Chinese name. Strawberry latent ringspot virus
Name. strawberrylatentringspotvirus
Abbreviation. SLRSV
Taxonomic position. Secoviridae Division, Wenzhou Satsuma dwarf virus genus (Sadwavirus)
For additional information, see Appendix Strawberry latent ringspot virus A.
4 principle of the method
Virion morphology, damage symptoms, serological properties and molecular characterization is the main basis for quarantine and identification of the virus, mining
By DAS-ELISA, RT-PCR, real-time fluorescence RT-PCR, immune electron microscopy and differential host reactions and other methods to identify and quarantine.
5 equipment, facilities, equipment and reagents
5.1 Equipment
Microplate reader, PCR, real-time PCR instrument, clean benches, electronic scales (sense of volume 0.001g), electrophoresis, electrophoresis tank, into the gel
Imaging systems, transmission electron microscope, water bath, high-speed refrigerated centrifuge, ultra-low temperature freezer (-80 ℃), autoclaves, ice maker, microwave
Furnace, a vortex.
5.2 Facilities
Plant quarantine nursery.
5.3 Appliances
Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL) and the corresponding RNase-free tips, RNase-off
Heart tube, PCR tubes, mortar, sample bags, labels, tweezers, magnifying glass and the like.
5.4 Reagents
5.4.1 Unless otherwise stated, all test reagents were analytical grade or biochemical reagents.
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