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GB/T 25879-2010: Determination of lysozyme in chicken egg white -- Spectrophotometry
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 25879-2010 | English | 169 |
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Determination of lysozyme in chicken egg white -- Spectrophotometry
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GB/T 25879-2010
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Basic data | Standard ID | GB/T 25879-2010 (GB/T25879-2010) | | Description (Translated English) | Determination of lysozyme in chicken egg white -- Spectrophotometry | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B43 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 7,721 | | Date of Issue | 1/10/2011 | | Date of Implementation | 6/1/2011 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Announcement of Newly Approved National Standards 2011 No. (No. 166 overall) 1 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Standard specifies spectrophotometric method eggs egg white lysozyme. This Standard is applicable to eggs and egg white lysozyme determination of viability. | GB/T 25879-2010: Determination of lysozyme in chicken egg white -- Spectrophotometry---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of lysozyme in chicken egg white. Spectrophotometry
ICS 65.020.30
B43
National Standards of People's Republic of China
Determination of egg white lysozyme
Issued on. 2011-01-10
2011-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee of animal husbandry.
This standard was drafted. Yangzhou University College of Animal Science and Technology.
The main drafters of this standard. Wang Jinyu, Xie Kai Zhou, Dai Guojun, Hou Qirui, Liu Dalin.
Determination of egg white lysozyme
1 Scope
This standard specifies the spectrophotometric method of egg white lysozyme.
This standard applies to the determination of lysozyme in egg white content and vitality to.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Egg eggwhite
Located in the semi-fluid colloidal substances egg yolk and eggshell endometrial film.
3.2
Lysozyme lysozyme
A special effect on the microbial cell walls of glycoside hydrolases.
4 Determination of the content of egg white lysozyme
4.1 Principle
Lysozyme maximum absorption peak at 281nm wavelength. Lysozyme standard solution containing 9g per liter of sodium chloride solution to dissolve and dilute
Interpretation into different gradient concentration, absorbance was measured at a wavelength of 281nm and standard curve obtained standard curve regression equation. After the sample
Processing, by lysozyme standard measuring method of measuring the absorbance of the sample, the sample is calculated from the standard curve regression equation based on the absorbance value
Lysozyme content.
4.2 Instrument
4.2.1 UV - visible spectrophotometer (wavelength range of 190nm ~ 900nm, wavelength uncertainty ± 0.3nm, wavelength repeatability ± 0.1nm).
4.2.2 electronic analytical balance (inductance 0.1mg, 1mg).
4.2.3 Centrifuge (maximum centrifugal force of 2325g, a 10mL centrifuge tube).
4.3 Reagents and materials
4.3.1 lysozyme standard, pure class distinctions, vitality 20000U/mg or so.
4.3.2 Sodium chloride, analytical grade.
4.3.3 of distilled water, according to GB/T 6682 execution.
4.3.4 medical gauze.
4.4 reagent preparation
4.4.1 0.9% sodium chloride solution. Weigh sodium chloride (4.3.2) 0.9g, was dissolved in distilled water and dilute to 100mL.
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