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SN/T 4818-2017: Determination of ractopamine, salbutamol, clenbuterol hydrochloride in edible animal for imported and export. Enzyme linked immunosorbent assay method
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Determination of ractopamine, salbutamol, clenbuterol hydrochloride in edible animal for imported and export. Enzyme linked immunosorbent assay method
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SN/T 4818-2017
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Basic data | Standard ID | SN/T 4818-2017 (SN/T4818-2017) | | Description (Translated English) | Determination of ractopamine, salbutamol, clenbuterol hydrochloride in edible animal for imported and export. Enzyme linked immunosorbent assay method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X22 | | Word Count Estimation | 7,764 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Regulation (derived from) | National Quality Inspection (2017) No. 337 | | Issuing agency(ies) | General Administration of Customs | SN/T 4818-2017: Determination of ractopamine, salbutamol, clenbuterol hydrochloride in edible animal for imported and export. Enzyme linked immunosorbent assay method
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(Determination of lycopamine, salbutamol and clenbuterol hydrochloride in edible animals by enzyme-linked immunosorbent assay)
People's Republic of China entry and exit inspection and quarantine industry standards
Import and export of food animals in ractopamine,
Determination of salbutamol and clenbuterol hydrochloride
Enzyme linked immunosorbent assay
Determinationofractopamine, salbutamol, clenbuterolhydrochlorideinedible
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Sun Mingjun, Sun Tao, Liang Guanghui, Liang Chengzhu, Zheng Xiaolong, Yue Zhiqin, Zhu Laihua, Xu Wei.
Import and export of food animals in ractopamine,
Determination of salbutamol and clenbuterol hydrochloride
Enzyme linked immunosorbent assay
1 Scope
This standard specifies the enzymes for the detection of ractopamine, salbutamol and clenbuterol residues in the plasma and urine of imported and exported food animals.
Combined immunosorbent assay.
This standard applies to the blood and urine of imported and exported poultry, ractopamine, salbutamol and clenbuterol hydrochloride
Rapid screening test, suitable for entry and exit inspection and quarantine.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Principle
Using horseradish peroxidase (HRP) and chemical methods to utilize the specific binding properties of antigen-antibodies and the efficient catalytic action of enzymes
Clenbuterol (CL) is coupled to form a horseradish peroxidase-labeled clenbuterol. The coated antibody on the solid phase carrier and the specific anti-gram
The Lentrol antibody binds to immobilize the anti-Clenbuter antibody, and then adds the Clenbuterol and horseradish peroxidase-labeled Clenbuterol to be tested.
They are competitively bound to the Clenbuterol antibody, washed with a color developing agent, and the amount of clenbuterol to be measured is measured based on the color change of the color developing agent. If
When the Clenbuterol is to be tested, the enzyme labeled with Clenbuter is less, the color of the developer is light, and vice versa. Visually or colorimetrically
The Clenbuterol content in the product, the optimal wavelength for colorimetry is 450 nm.
The principle of salbutamol and ractopamine is the same as that of Clenbuterol.
4 reagents and materials
4.1 1mol/L sodium hydroxide. See Appendix A for preparation. The test water should meet the requirements of GB/T 6682.
4.2 Ethyl acetate. analytically pure.
4.3 β-glucuronidase. β-glucuronidase 111000 U/mL.
4.4 Potassium phosphate buffer.
4.5 Borate buffer.
4.6 coating buffer.
4.7 Wash buffer.
4.8 Blocking buffer.
4.9 Dilution buffer.
4.10 Horseradish peroxidase labeled conjugate dilution.
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