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SN/T 4810-2017: Determination of β-lactams residues of live animals for import and export. Radio-receptor assay method
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| SN/T 4810-2017 | English | 149 |
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Determination of β-lactams residues of live animals for import and export. Radio-receptor assay method
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SN/T 4810-2017
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Basic data | Standard ID | SN/T 4810-2017 (SN/T4810-2017) | | Description (Translated English) | Determination of ��-lactams residues of live animals for import and export. Radio-receptor assay method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X22 | | Word Count Estimation | 5,547 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Regulation (derived from) | National Quality Inspection (2017) No. 337 | | Issuing agency(ies) | General Administration of Customs | SN/T 4810-2017: Determination of β-lactams residues of live animals for import and export. Radio-receptor assay method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of β - lactam residues in foodstuffs and animals - Radiation receptor analysis)
People's Republic of China entry and exit inspection and quarantine industry standards
Import and export of edible animal β-lactam drugs
Determination of residual amount
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Meng Rizeng, Kang Mingqin, Song Yuzhu, Liu Wei, Liu Jinhua, Zhang Xuguang, Qu Hui, Wang Jialu, Shi Jianping.
Import and export of edible animal β-lactam drugs
Determination of residual amount
1 Scope
This standard specifies the detection method for β-lactam antibiotic residues in blood and urine of imported and exported food animals.
This standard is applicable to 6 kinds of antibiotics of β-lactam in import and export food activities (penicillin G, amoxicillin, ampicillin, cephalosporin
Screening for the determination of residual amounts ofmycin, ceftiofur, and o-chloropenicillin.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Principle
The principle of the method is the binding reaction of a drug functional group to a microbial receptor site, and these sites are shared with a certain class of antibiotics.
With functional group correlation, this specificity of the applied receptor allows for multi-residue analysis of a certain class of antibiotics. When the sample contains β-lactam
In the case of an amine antibiotic, the β-lactam antibiotic residue in the sample binds to the binding site of the corresponding receptor in the receptor reagent, thereby preventing
[14C] Labeled penicillin G binds to the receptor reagent site. The higher the content of β-lactam in the sample, the more competitive binding sites.
The [14C] labeled penicillin G binds less, and the cpm value of the [14C] content in the sample is determined by a liquid scintillation counter (countper-
The lower the minute, or the number of pulses per minute. The amount of β-lactam antibiotic residues in the sample is inversely proportional to the cpm value.
4 reagents and materials
4.1 β-lactam antibiotic Charm-II assay kit 1
(MRLSequentialAssay). The tablet is identified as P.
1) This information is given for the convenience of users of this standard and does not imply endorsement of the operation of a product. If other products have steps
Different, must be used after experimental evaluation.
4.2 Negative control sample. A matrix concentrated dry powder that has been confirmed to contain no β-lactam antibiotics and stored at 2 ° C ~ 6 ° C.
4.3 MSU multi-antibiotic concentration standard. contains penicillin G, amoxicillin, ampicillin, cephalosporin, ceftiofur, penicillin
A standard for a variety of antibiotics. Dissolve in 10mL water when used, shake well, and prepare MSU various antibiotic standard solution (including
The penicillin G concentration was 1000 μg/kg).
4.4 MSU extraction buffer concentrated dry powder. dissolve in 1000mL water when used, can be stored at 2 ° C ~ 6 ° C for 2 months.
4.5 M2 buffer concentrated dry powder. use concentrated concentrated powder dissolved in 50mL water when used, formulated into M2 buffer, can be stored at 2 ° C ~ 6 ° C
Save for 2 months.
4.6 Scintillation fluid.
4.7 Silicoborate glass test tubes and test tube stoppers.
4.8 stoppered plastic centrifuge tube. 50mL.
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