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SN/T 2614-2010: Detection and identification of Greeneria uvicola (Berk. and Curtis) Punithalingam
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2614-2010 | English | 209 |
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Detection and identification of Greeneria uvicola (Berk. and Curtis) Punithalingam
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SN/T 2614-2010
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Basic data | Standard ID | SN/T 2614-2010 (SN/T2614-2010) | | Description (Translated English) | Detection and identification of Greeneria uvicola (Berk. and Curtis) Punithalingam | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 8,813 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry and exit of bitter rot of grapes plant quarantine in quarantine and identification of bacteria. This standard applies to entry and exit of grapes and grape cuttings bitter rot in quarantine and identification. | SN/T 2614-2010: Detection and identification of Greeneria uvicola (Berk. and Curtis) Punithalingam
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Greeneria uvicola (Berk.
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Grape quarantine and identification of Bitter Rot
DetectionandidentificationofGreeneriauvicola (Berk.
Punithalingam
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Zhejiang Exit Inspection and Quarantine, the People's Republic of China
Exit Inspection and Quarantine and the State of Xinjiang.
The main drafters of this standard. Du Hongzhong, products Wu Shan, Wu Zhiyi, Wang Wen-cheng, Yan Jin.
Grape quarantine and identification of Bitter Rot
1 Scope
This standard specifies the method of entry and exit quarantine and identification of plant quarantine grapes bitter rot bacteria.
This standard applies to entry and exit quarantine and identification of grape berry and grape cuttings in bitter rot bacteria.
Principle 2
Grapes bitter rot symptoms on host bacteria, morphology and molecular biological characteristics of the main basis for the development of this standard.
3 apparatus and appliances
3.1 Instrument
Microscope (with measurements), stereo microscope, clean benches, light biological incubator, scales, autoclaves, high-speed desktop
Centrifuges, pH meter, water bath, PCR amplification device, electrophoresis, gel imager.
3.2 Appliances
Beakers, flasks, graduated cylinder, test tubes, Petri dishes (diameter 9cm), slides, cover slips, alcohol lamp, plastic pestle, pipettes, pipette
Pipette tips, centrifuge tubes, liquid nitrogen tank.
4 Reagents and culture medium
4.1 Reagents
Agar, potato, dextrose, agarose, sterile double distilled water, liquid nitrogen, Tris-HCl, magnesium chloride, hydrochloric acid, EDTA, sodium acetate,
Sodium, potassium chloride, Tris, CTAB, TAE, ethanol, phenol, ethidium bromide (EB), chloroform, isopropanol, isoamyl alcohol, proteinase K,
TaqDNA polymerase, dNTP, DNA molecular weight Marker, PCR primers PMA-1 and PMA-2, especially real-time PCR
Specific primers PMA-P1, PMA-P2 probe and PMA-Probe-457.
4.2 Medium
Potato dextrose medium (PDA). potato 200g, dextrose 20g, Agar 20g, Distilled water 1000mL, sterilization.
5 identification methods
5.1 Symptom Check
Disease symptoms in Appendix A.
5.2 Isolation
Healthy and junction cut side (take the skin of ripe berry) is 5mm ~ 10mm sample, 70% alcohol for 10s ~ 30s, put
On PDA medium, 24 ℃ 12h light/12h dark for 1 to 2 weeks until fungal colonies appear, then go to the PDA purification.
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