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SN/T 2434-2010: Protocol of quarantine for Bonamiosis in shellfish
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| SN/T 2434-2010 | English | 319 |
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Protocol of quarantine for Bonamiosis in shellfish
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SN/T 2434-2010
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Basic data | Standard ID | SN/T 2434-2010 (SN/T2434-2010) | | Description (Translated English) | Protocol of quarantine for Bonamiosis in shellfish | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 12,130 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Quoted Standard | GB/T 6682; GB/T 18088; SN/T 1193 | | Adopted Standard | Aquatic Animal Disease Diagnostic Manual (OIE) 2006/2.2.1/INFECTION WITH BONAMIA OSTREAE and 2.2.1/INFECTION WITH BONAMIA EXITIOSA, NEQ | | Regulation (derived from) | National Quality Inspection [2010] No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the package Lamy echinococcosis quarantine sample collection, tissue blot, PCR detection of pathology and the rules. This standard applies to oysters and other aquatic animals and their products carry the oyster bag Lamy insects (Bonamia ostreae), package Lamy protozoa (Bonamia sp) and other quarantine pests Lamy package can also be used farm package Lamy echinococcosis monitored. | SN/T 2434-2010: Protocol of quarantine for Bonamiosis in shellfish---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of quarantine for Bonamiosis in shellfish
Exit inspection and quarantine industry standard book People's Republic of China
Shellfish package Lamy echinococcosis quarantine Technical Specifications
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard is not equivalent to the International Organization for Animal Health (OIE) Manual prepared by the aquatic animal disease diagnosis (2006 edition) 2.2.1
INFECTIONWITHBONAMIAOSTREAE and 2.2.1INFECTION WITHBONAMIAEXITIOSA
And other relevant sections.
The Standard Appendix A and Appendix B are normative, Appendices C and D are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard drafting unit. China Academy of Inspection and Quarantine.
Participated in the drafting of this standard. People's Republic of China SZCIQ, People's Republic of China Shandong Entry-Exit Inspection and
Quarantine Bureau, People's Republic of China Fujian Entry-Exit Inspection and Quarantine Huangdao People's Republic of China.
The main drafters of this standard. Wushao Jiang, Lin Xiangmei, Orientin Liu, Yue Zhiqin, Liu Zheng Teng, Li Xifeng, Han Xueqing, Jia Guangle, Meilin,
Caixia.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Shellfish package Lamy echinococcosis quarantine Technical Specifications
1 Scope
This standard specifies the sample collection bag when Lamy echinococcosis quarantine, tissue blot, histopathology and PCR procedures detection.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
SN/T 1193 genetic analysis and detection laboratory technical requirements
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Connective tissue, gills, viscera or mantle epithelium caused serious parasitic diseases of aquatic animals.
3.2
Small subunit ribosomal DNA, the species more conservative DNA fragments, often used as the basis for the identification and classification of species.
4 Overview
Lamy package echinococcosis is a serious harm to the world's major diseases shellfish aquaculture and shellfish also OIE provisions must be reported one disease. Pathogen
Australia OYSTER B. sp. Natural hosts include the European oyster, New Zealand oysters, oyster Argentina, Portugal oysters, quail snails China,
We can not infect the giant Pacific oyster, Crassostrea virginica, mussels and other shellfish. After infection symptoms mainly closed muscular atrophy, fading into yellow, graininess
Leukocyte, appear white small ulcers or perforated ulcer, blood cells rupture, blood oozing. Gonad, mantle, gill damage in large areas,
And ultimately death.
5 material
Unless otherwise specified, the use of chemical reagents were of analytical grade. Test water required to achieve the GB/T 6682 in a water requirements.
5.1 coated with aminoalkyl silane (aminoalkylsilane) slides the adhesive.
5.2 Giemsa, hematoxylin - eosin staining solution, eosin dye, back blue liquid, Davidson's fixative, etc., preparation see Appendix A.
Reagents 5.3 DNA extraction require organizations or other commercially available DNA extraction kit, see Appendix B.
5.4 PCRbuffer, magnesium, dNTP (2.5mmol/L, or 10mmol/L), the sample DNA polymerase buffer, DNAMaker, electrophoresis
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