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SN/T 2074-2008: Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in familiar edible fungi
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2074-2008 | English | 209 |
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Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in familiar edible fungi
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SN/T 2074-2008
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Basic data | Standard ID | SN/T 2074-2008 (SN/T2074-2008) | | Description (Translated English) | Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in familiar edible fungi | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 8,835 | | Date of Issue | 2008-04-29 | | Date of Implementation | 2008-11-01 | | Quoted Standard | GB/T 6682; SN/T 1204 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 7 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the major edible fungi GMO inspection sampling, sample preparation, Qualitative PCR detection methods. This standard applies to the main mushroom (mushroom, red mushroom, Agaricus, shiitake mushrooms, straw mushrooms, Boletus, Coprinus, mushroom, Agaricus bisporus, tripe mushroom, Pleurotus, a small mushroom, oyster mushrooms, Hericium, citrinopileatus, tea mushroom, bamboo fungus, black fungus, white fungus, etc.) in GMO detection. | SN/T 2074-2008: Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in familiar edible fungi
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Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in familiar edible fungi
Exit inspection and quarantine industry standard book People's Republic of China
Genetically Modified qualitative Edible Fungus
PCR detection method
Posted 2008-04-29
2008-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine.
The main drafters of this standard. Chenwen Bing, Shaobi Ying, Jiang Shuxun, Lishou Song, Zhu Xiaonan, Guo Lixin, Yang Jie.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Genetically Modified qualitative Edible Fungus
PCR detection method
1 Scope
This standard specifies the sampling, sample preparation, qualitative PCR detection methods in the Mushroom GMO testing.
This standard applies to the major edible fungus (mushroom, red mushroom, Agaricus blazei, mushrooms, mushroom, Boletus, Coprinus, mushroom, Agaricus bisporus,
Pork bellies mushroom, Pleurotus, small mushroom, oyster mushrooms, Hericium, Pleurotus, Agrocybe, bamboo fungus, black fungus, white fungus, etc.) in the GMO
Testing.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
SN/T 1204 GMO plants and their processed products in real-time PCR qualitative test methods
3 Abbreviations
The following abbreviations apply to this standard.
3.1
Derived from the Agrobacterium tumefaciens nopaline synthase gene.
3.2
3.3
β- glucuronidase gene (as a plant gene expression of a reporter gene).
3.4
Neomycin from E. coli K12 strain 3'-phosphotransferase Ⅱ genes.
3.5
Coding 18SrRNA (ribosomal) of DNA.
Principle 4
After extraction of the sample DNA, for common exogenous gene transgenic edible fungi of the introduced gene sequence primers PCR
Technology, DNA fragments of exogenous gene was specifically amplified, according to the experimental results, it is determined whether a sample containing the edible fungus exogenous gene
ingredient.
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