SN/T 2055: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2055-2016 | English | 559 |
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Detection and identification of Cowpea severe mosaic virus
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SN/T 2055-2016
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| SN/T 2055-2008 | English | 359 |
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Identification of cowpea severe mosaic virus
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SN/T 2055-2008
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PDF similar to SN/T 2055-2016
Basic data | Standard ID | SN/T 2055-2016 (SN/T2055-2016) | | Description (Translated English) | Detection and identification of Cowpea severe mosaic virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 14,169 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Older Standard (superseded by this standard) | SN/T 2055-2008 | | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | | Issuing agency(ies) | General Administration of Customs | SN/T 2055-2016: Detection and identification of Cowpea severe mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Cowpea severe mosaic virus
China's entry-exit inspection and quarantine industry standards
Replacing SN/T 2055-2008
Kidney bean heavy mosaic virus quarantine and identification method
Published on.2016-08-23
2017-03-01 Implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces SN/T 2055-2008 “Quarantine Heavy Mosaic Virus Quarantine and Identification Methods”, compared with SN/T 2055-2008, mainly
The changes are as follows.
--- Increased routine RT-PCR detection methods;
--- Added real-time fluorescence RT-PCR detection method.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This standard is drafted by. China Academy of Inspection and Quarantine, People's Republic of China, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Chinese
Republic of China Xiamen Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Guifen, Kong Jun, Li Bin, Ma Jie, Chen Qing, Wei Meisheng, Zhang Yongjiang.
The previous releases of the standard replaced by this standard are.
---SN/T 2055-2008.
Kidney bean heavy mosaic virus quarantine and identification method
1 Scope
This standard specifies the quarantine and identification methods for cowpea heavy mosaic virus in plant quarantine.
This standard applies to the quarantine and identification of cowpea heavy mosaic virus in leguminous propagation materials (seeds and plants).
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
3 Basic information
Scientific name. Cowpeaseveremosaicvirus
Abbreviation. CPSMV
Taxonomic status. Associated Secoviridae, Comovirinae, Cowpea Mosaic Virus
(Comovirus).
See Appendix A for additional information on this virus.
4 Methodology
According to the specific reaction between cow bean heavy mosaic virus and antibody, ELISA test of plant samples. According to the nucleic acid of the virus
PCR-specific detection of the sequence, determination of the result by electrophoretic band size, design of specific primers based on the nucleic acid sequence of the virus, and
Fluorescent probes are used to determine the result of the detected fluorescence signal.
5 Equipment and reagents
5.1 Instruments and Equipment
Microplate reader, balance (sensibility, 1/10000g), pH meter, micro-juice extractor, PCR machine, real-time fluorescence PCR apparatus, electrophoresis apparatus, electrophoresis tank,
Gel imaging system, isolation greenhouse, constant temperature water bath, low temperature refrigerator, etc.
Micropipettes (2 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL), enzyme-linked plates, mortars, centrifuge tubes, pots, and sterilized soils.
5.2 Reagents
For enzyme-linked immunosorbent assay reagents, see Appendix B, B.1, RT-PCR test reagents in Appendix C, C.1, Real-time fluorescence RT-PCR assay.
The test agent is shown in Appendix D, D.1.
Test water should meet the requirements of GB/T 6682.
6 Sample preparation
6.1 Seeds
Pick up the deformed, immature seeds and randomly selected seeds and sow them in sterilized soil. After the young leaves are grown, separate the plants that express the symptoms.
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