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SN/T 2033-2007: Protocol of micro-complement fixation test for enzootic abortion of ew
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2033-2007 | English | 359 |
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Protocol of micro-complement fixation test for enzootic abortion of ew
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SN/T 2033-2007
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Basic data | Standard ID | SN/T 2033-2007 (SN/T2033-2007) | | Description (Translated English) | Protocol of micro-complement fixation test for enzootic abortion of ew | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 11,191 | | Date of Issue | 2007-12-24 | | Date of Implementation | 2008-07-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National-accreditation-Science [2011] 63 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the abortion of sheep endemic disease micro complement fixation test the operation of the technical requirements. This standard applies to sheep endemic disease serological diagnosis of miscarriage. | SN/T 2033-2007: Protocol of micro-complement fixation test for enzootic abortion of ew---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of micro-complement fixation test for enzootic abortion of ew
Exit inspection and quarantine industry standard book People's Republic of China
Sheep endemic abortion disease micro complement fixation test
Practice
Posted 2007-12-24
2008-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The standard Appendix C, Appendix D is a normative appendix, Appendix A, Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Wang Yuling, Houyan Mei, JIANG, Zhao Xiangping, Dong Zhizhen, Huolei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Sheep endemic abortion disease micro complement fixation test
Practice
1 Scope
This standard specifies the sheep endemic abortion disease micro complement fixation test operation technical requirements.
This standard applies to sheep endemic abortion disease serological diagnosis.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (neq ISO 3696)
3 Reagents and materials
3.1 Reagents
3.1.1 Test with distilled water to GB/T 6682 in the third grade water.
3.1.2 Diluent. saline.
3.1.3 complement fixation antigen. according to the instructions to use, shake well before use.
3.1.4 positive control serum. prior to use distilled water to a predetermined volume.
3.1.5 Negative control serum. prior to use distilled water to a predetermined volume.
3.1.6 hemolysin. prior to use distilled water to a predetermined volume.
3.1.7 Complement. prior to use distilled water to a predetermined volume.
3.1.8 Sheep blood. choose healthy adult sheep, sterile jugular vein blood, Apgar 1.2 parts 1 part liquid blood, mix, within 4 ℃ refrigerator stable 3d ~
5d after use.
3.2 Material
3.2.1 Glass tube. 10mm × 75mm, round, clean.
3.2.2 test tube rack.
3.2.3 micropipette.
3.2.4 Continuous adjustable pipettes.
3.2.5 96 "U" shaped microplates.
3.2.6 96-well flat bottom () microtiter plates.
3.2.7 horizontal centrifuge rotor.
3.2.8 ELISA plate reader.
3.3 was tested serum
3.3.1 blood collection and separation
By conventional methods and separating blood serum. Serum should be fresh, protein-free floc, non-hemolytic, no smell of corruption.
3.3.2 transport and store serum
Serum to prevent freezing, if not sent to the laboratory within 3d, serum required refrigerated transport method. Serum should be put into the 4 ℃ refrigerator.
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