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SN/T 1155-2002 English PDF

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SN/T 1155-2002: Plant quarantine. Methods for quarantine and identification of Peronosclerospora (Ito) C.G. Shaw
Status: Valid
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SN/T 1155-2002English449 Add to Cart 3 days [Need to translate] Plant quarantine. Methods for quarantine and identification of Peronosclerospora (Ito) C.G. Shaw Valid SN/T 1155-2002

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Basic data

Standard ID SN/T 1155-2002 (SN/T1155-2002)
Description (Translated English) Plant quarantine. Methods for quarantine and identification of Peronosclerospora (Ito) C.G. Shaw
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Word Count Estimation 14,168
Date of Issue 2002-11-25
Date of Implementation 2003-05-01
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the maize downy mildew phytosanitary quarantine and identification methods. This standard applies to maize, sugarcane, sorghum, corn and other hosts in the quarantine and identification of downy mildew.

SN/T 1155-2002: Plant quarantine. Methods for quarantine and identification of Peronosclerospora (Ito) C.G. Shaw


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Plant quarantine.Methods for quarantine and identification of Peronosclerospora (Ito) CGShaw People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Phytosanitary Method for quarantine and identification of corn downy mildew Released on November 11,.2002 2003-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Content

Foreword I 1 Scope 1 2 Principle 1 3 instruments and appliances 1 4 reagents and materials 1 5 On-site quarantine and sampling 2 6 Laboratory test 3 7 result evaluation 5 8 sample storage 5 Appendix A (informative) Corn downy mildew host range 6 Appendix B (informative) DNA extraction, amplification and electrophoresis 7 Appendix C (Normative) PCR Amplification Primers 9 Appendix D (informative) Maize downy mildew oospore, conidia, conidiophores Figure 10

Foreword

Appendix C of this standard is a normative appendix, Appendix A, Appendix B, and Appendix D are informative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Li Defu, Guo Qiongxia, Chen Yan, Zhang Guangzhi, Zhang Xiaoyan. This standard is the industry standard for inspection and quarantine issued for the first time. Phytosanitary Method for quarantine and identification of corn downy mildew

1 Scope

This standard specifies the quarantine and identification methods for corn downy mildew in plant quarantine. This standard applies to the quarantine and identification of corn downy mildew in corn, sugar cane, sorghum and other hosts.

2 Principle

Other plants (see Appendix A). In general, the downy mildew of corn causes systemic infection, sometimes causing local infection, infected jade. The leaves of the rice showed obvious chlorosis at the boundary, and brown necrotic streaks formed later. Leaf surface disease at a temperature of 17 ° C to 29 ° C under ambient humid conditions The spot grows a layer of white mildew, namely the conidia and spore stalk of the downy mildew of corn. The pathogen conidia and spore stalk morphology, conidia The characteristics of germination, oosporogenesis, and PCR-specific reaction are the characteristics of the identification of downy mildew of corn.

3 instruments and appliances

3.1 Microscope, stereo microscope. 3.2 PCR instrument, electrophoresis instrument. 3.3 Freezer high speed centrifuge, benchtop microcentrifuge. 3.4 Ordinary refrigerator, low temperature refrigerator, constant temperature and humidity incubator. 3.5 Ordinary balance (sensitivity is 1/100). 3.6 Ultra-clean workbench. 3.7 Adjustable micro sampler. 3.8 Eppendorf tube (1.5 mL, 0.5 mL), OakRidge centrifuge tube (30 mL). 3.9 scorpion, flat head toothpick. 3.10 Slides, coverslips, and petri dishes (15 cm to 20 cm in diameter). 3.11 Aluminum platinum paper or Parafilm film.

4 reagents and materials

4.1 Tris(hydroxymethyl)aminomethane (C4H11NO3) 4.2 Ethylenediaminetetraacetic acid (C10H12N2O8) 4.3 sodium lauryl sulfate [(CH2)12OSO3Na] 4.4 2-mercaptoethanol (C2H6OS) 4.5 Isopropyl alcohol (C3H8O) 4.6 Isoamyl alcohol (C5H10O) 4.7 Ethanol (CH3CH2OH) 4.8 Bromophenol Blue (C19H10O5Br4S)

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