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SN/T 1135.3-2016: Detection and identification of Potato mop-top virus
Status: Valid SN/T 1135.3: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1135.3-2016 | English | 329 |
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Detection and identification of Potato mop-top virus
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SN/T 1135.3-2016
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| SN/T 1135.3-2003 | English | 599 |
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3 days [Need to translate]
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Quarantine methods for identification of potato mop-top Pomovirus
| Obsolete |
SN/T 1135.3-2003
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PDF similar to SN/T 1135.3-2016
Basic data | Standard ID | SN/T 1135.3-2016 (SN/T1135.3-2016) | | Description (Translated English) | Detection and identification of Potato mop-top virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 14,178 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Older Standard (superseded by this standard) | SN/T 1135.3-2003 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs | SN/T 1135.3-2016: Detection and identification of Potato mop-top virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Potato mop-top virus
People's Republic of China Exit Inspection and Quarantine standards
Replacing SN/T 1135.3-2003
Potato top broom virus quarantine and identification method
2016-12-12 released
2017-07-01 implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1135 series of standards is divided into 13 parts.
--- Part 1. Potato disease detection and identification of cancer;
--- Part 2. Potato yellow dwarf virus quarantine and identification methods;
--- Part 3. Potato top broom virus quarantine and identification methods;
--- Part 4. Potato powdery mildew quarantine and identification methods;
--- Part 5. Potato ringworm bacteria quarantine and identification methods;
--- Part 6. Potato virus pathogen quarantine and identification methods;
--- Part 7. Potato virus A quarantine and identification methods;
--- Part 8. Potato asymptomatic bacteria quarantine and identification methods;
--- Part 9. Potato bacterial wilt quarantine and identification methods;
--- Part 10. Potato virus V quarantine and identification methods;
--- Part 11. Potato pathogens quarantine and identification methods;
--- Part 12. Potato M virus quarantine and identification methods;
--- Part 13. Potato virus Y quarantine and identification methods.
This part of the SN/T 1135 Part 3.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This Part replaces SN/T 1135.3-2003 "potato broom top virus quarantine and identification methods."
This section compared with the SN/T 1135.3-2003, the main technical differences are as follows.
--- Removed "Introduction";
--- Removed the "Terms and definitions" chapter;
--- Added "potato broom top virus basic information" chapter;
--- Removed "on-site quarantine and sampling" chapter;
--- Added "test method" section;
--- Modify the RT-PCR detection method.
Please note that some of this document may be patentable. The publication structure of this document does not bear the responsibility of identifying these patents.
This part of the national certification and accreditation supervision and management committee proposed and centralized.
This part of the drafting unit. China Inspection and Quarantine Science Institute.
The main drafters of this section. Li Mingfu, Zhang Yongjiang, Ding Xiaolan, Xiang Ning, Li Guifen, Wei Meisheng, Chen Zhinan, Huang Wensheng, Zhang Chengliang.
This standard replaces the standards previously issued as.
--- SN/T 1135.3-2003.
Potato top broom virus quarantine and identification method
1 Scope
This section provides the basic principles and methods of potato broom top virus quarantine and identification.
This section applies to all entry seed, potato products, tissue culture seedlings and virus-free potato germplasm in the potato broom top virus quarantine test
Field investigation of diseases caused by potato broom top virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 1840 plant virus immune electron microscopy detection method
SN/T 2122 Quarantine sampling of entry and exit plants and plant products
3 potato top broom virus basic information
Chinese name. Potato top broom virus
Scientific name .Patatomop-topvirus
Abbreviation. PMTV
Taxonomic status Virgaviridae Potato, genus Pomovirus.
See Appendix A for additional information on potato broom top virus.
4 method principle
The serological characteristics, molecular biological characteristics and biological characteristics of potato broom top virus are the main basis for quarantine and identification.
5 equipment, appliances and reagents
5.1 Equipment
Electronic analytical balance (0.0001g), small centrifuge, desktop refrigerated centrifuge, constant temperature water bath, microplate reader, general PCR machine, electrophoresis
System, pH meter, gel imaging system, 4 ° C refrigerator, ultra clean bench, ultra low temperature refrigerator at -80 ° C, autoclave, ice maker, vortex oscillation
Microwave oven, electron transmission microscope and the like.
5.2 appliances
Adjustable pipette (2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL, 5000 μL) and corresponding RNase-free tip,
RNase-free centrifuge tubes, PCR tubes, mortars, sample bags, labels and more.
5.3 Reagents
Except for special instructions, all experimental reagents are of analytical grade.
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