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SN/T 1135.8-2017: Detection and identification of Phoma foveata Foister
Status: Valid SN/T 1135.8: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1135.8-2017 | English | 259 |
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Detection and identification of Phoma foveata Foister
| Valid |
SN/T 1135.8-2017
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| SN/T 1135.8-2009 | English | 239 |
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3 days [Need to translate]
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Quarantine identification of Phoma exigua var.foveata
| Obsolete |
SN/T 1135.8-2009
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PDF similar to SN/T 1135.8-2017
Basic data | Standard ID | SN/T 1135.8-2017 (SN/T1135.8-2017) | | Description (Translated English) | Detection and identification of Phoma foveata Foister | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 11,192 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Older Standard (superseded by this standard) | SN/T 1135.8-2009 | | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the quarantine and identification methods for potato gangrene. This section applies to the quarantine and identification of potato gangrene bacteria carried by all imported potato tubers. | SN/T 1135.8-2017: Detection and identification of Phoma foveata Foister---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phoma foveata Foister
People's Republic of China entry and exit inspection and quarantine industry standards
Replace SN/T 1135.8-2009
Method for quarantine and identification of potato gangrene
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 1135 is divided into 13 parts;
--- Part 1. Quarantine and identification methods for potato cancer;
--- Part 2. Quarantine and identification methods for potato yellowing dwarf virus;
--- Part 3. Quarantine and identification methods for potato dome virus;
--- Part 4. Quarantine and identification methods for potato black powder bacteria;
---Part 5. Quarantine and identification methods for potato ring rot;
--- Part 6. Quarantine and identification methods for potato rot fungus;
--- Part 7. Potato A virus quarantine identification method;
--- Part 8. Quarantine and identification methods for potato gangrene;
---Part 9. Quarantine and identification methods for potato bacterial wilt;
--- Part 10. Potato V virus quarantine identification method;
--- Part 11. Quarantine and identification methods for potato skin spot bacteria;
--- Part 12. Quarantine and identification methods for potato M virus;
--- Part 13. Potato Y virus quarantine identification method.
This part is the eighth part of SN/T 1135.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
This part replaces SN/T 1135.8-2009 "Potato gangster quarantine identification method".
The main technical differences between this part and SN/T 1135.8-2009 are as follows.
--- Supplemented the culture traits and spore morphology in biological characteristics, and increased the molecular biology detection method.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Gansu Entry-Exit Inspection and Quarantine
Bureau of Plant Protection, Gansu Academy of Agricultural Sciences.
The main drafters of this section. Li Xin, Wen Zhaohui, Heidol, He Suqin, Liu Wei, Wang Xiufen, Wang Youfu.
The previous versions of the standards replaced by this section are.
---SN/T 1135.8-2009.
Method for quarantine and identification of potato gangrene
1 Scope
This part of SN/T 1135 specifies the quarantine and identification methods for potato gangrene.
This section applies to the quarantine and identification of potato gangrene bacteria carried by all imported potato tubers.
2 Basic information
Chinese name. potato gangrene
English name. potatogangrene, gangreneofpotato, tuberrot, tubergangrene, dryrotofpotato, leavespot
Oftobacco,leafspotofbean
Having sex. Boeremiafoveata (Foister) Aveskamp, Gruyter
Asexuality. PhomafoveataFoister
Synonym. Phomaesiguavar.foveata(Foister)Boerema
Phomasolanicolavar.foveata (Foister) Malc.
Phomasolanicolaf.foveata (Foister) Malc.
Classification status. KingdomFungi, Ascomycota, Dothideomycetes, Phytophthora
Pleosporales, Didymelaceae family, Boeremia genus.
Route of transmission. The transportation of infected potato seed potatoes is the main way to spread long-distance. Insects and nematodes benefit pathogens
Local spread.
See Appendix A for additional information on potato gangrene.
3 Principle of the method
In this part, the main hazard characteristics of the pathogen on the potato, the culture characteristics, pathological characteristics and specific PCR reaction of the pathogen
The basis for identifying potato gangrene.
4 instruments and main reagents
4.1 Instrumentation
Electronic balance (sensing 1/10000g), dissecting mirror, microscope, ultra-clean workbench, temperature-controlled incubator, autoclave, low-temperature refrigerator, general
Through the refrigerator, tweezers, measuring cylinders, petri dishes, pipettes, horizontal gel electrophoresis devices, PCR instruments, gel imaging systems, high-speed refrigerated centrifuges and so on.
4.2 Primary reagents and media
Taq DNA polymerase, DL2000, dNTP, 10×PCR buffer, absolute ethanol, sodium hypochlorite, ammonia, potato agar culture
Base (PDA), oatmeal medium (OA), maltose agar medium (MEA) (see Appendix B for medium formulation).
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