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HYT0295-2020: (Technical Regulations for Molecular Identification of Sponge Species)
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| HY/T 0295-2020 | English | 209 |
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(Technical Regulations for Molecular Identification of Sponge Species)
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HY/T 0295-2020
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Basic data | Standard ID | HY/T 0295-2020 (HY/T0295-2020) | | Description (Translated English) | (Technical Regulations for Molecular Identification of Sponge Species) | | Sector / Industry | Marine Industry Standard (Recommended) | | Classification of Chinese Standard | A45 | | Word Count Estimation | 9,917 | | Date of Issue | 2020-06-28 | | Date of Implementation | 2020-10-01 | | Regulation (derived from) | Ministry of Natural Resources Announcement No. 46 (2020) | | Issuing agency(ies) | Ministry of Natural Resources of the People's Republic of China | HYT0295-2020: (Technical Regulations for Molecular Identification of Sponge Species)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical Regulations for Molecular Identification of Sponge Species)
ICS 07.060
A45
People's Republic of China Marine Industry Standard
Technical Regulations for Molecular Identification of Sponge Species
2020-06-28 released
2020-10-01 implementation
Issued by the Ministry of Natural Resources of the People's Republic of China
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the Ministry of Natural Resources of the People's Republic of China.
This standard is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283).
Drafting organizations of this standard. Dalian Ocean University, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian Jinpu New District Agricultural and Rural Development Service Center.
The main drafters of this standard. Fu Wantao, Cao Xupeng, Du Mengmeng, Su Yanming, Wang Gang, Wu Mengjia, Zhang Shijie, Li Dongzi, Shen Si,
Ding Xiaohan, Wang Xuan, Xiao Yifan, Wang Shaojie, Lu Yanli, Li Jingying.
Technical Regulations for Molecular Identification of Sponge Species
1 Scope
This standard specifies the principles and methods for molecular biological identification of sponge species, and the collection and storage of sponge samples.
This standard applies to sponge sample collection, preservation and molecular biological methods for species identification.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 12763.6-2007 Marine Survey Specification Part 6.Marine Living Resources Survey
GB/T 30744-2014 Technical Specification for Pretreatment of Deep Sea Microbial Samples
HY/T 058-2010 Marine Survey Observation and Monitoring Archives Business Specification
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
sponge
At present, the oldest and lowest multicellular animals known to still survive, constitute the porous animal phylum alone in the animal kingdom.
3.2
DNA barcode
A relatively short DNA fragment that can represent the species, is standard, has sufficient variation, is easy to amplify, and is used to quickly and accurately identify and identify the species.
3.3
Sponge barcode
An expression form of the conserved gene sequence information of sponge.
3.4
18SrRNA gene
The DNA sequence encoding the 18SrRNA gene.
3.5
Cytochrome Oxidase Subunit I
DNA sequence encoding mitochondrial cytochrome oxidase subunit I gene.
Note. Hereinafter referred to as COI.
3.6
Polymerase chain reaction
The DNA template is denatured to single-stranded at high temperature. At an appropriate temperature and in a buffer, the two primers are on the two strands of the template DNA.
A complementary sequence of the anneal occurs, and then four dNTPs are used as substrates under the catalysis of DNA polymerase to extend the annealing primer, such as
This repeated denaturation, annealing and extension make the DNA fragment between the two primer sequences amplify geometrically.
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