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GB/T 34750-2025: Diagnostic techniques for Glasser's disease
Status: Valid GB/T 34750: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 34750-2025 | English | 439 |
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Diagnostic techniques for Glasser's disease
| Valid |
GB/T 34750-2025
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| GB/T 34750-2017 | English | 359 |
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Detection methods for haemophilus parasuis
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GB/T 34750-2017
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PDF similar to GB/T 34750-2025
Basic data | Standard ID | GB/T 34750-2025 (GB/T34750-2025) | | Description (Translated English) | Diagnostic techniques for Glasser's disease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 22,215 | | Date of Issue | 2025-01-24 | | Date of Implementation | 2025-08-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 34750-2025: Diagnostic techniques for Glasser's disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220
CCSB41
National Standard of the People's Republic of China
Replace GB/T 34750-2017
Glasser's disease diagnosis technology
Released on 2025-01-24
2025-08-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface III
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Clinical diagnosis 1
6 Sample collection, processing and storage 2
7 Bacterial isolation and identification 3
8 Nested PCR Detection 5
9 Real-time fluorescence PCR detection 7
10 Indirect ELISA 8
11 Comprehensive judgment 9
Appendix A (Normative) Preparation of Isolation, Culture and Biochemical Identification Reagents 11
Appendix B (Informative) Isolation and Identification of Glassera parasuis Figure 12
Appendix C (Normative) Primer sequences used for nested PCR and real-time fluorescence PCR 13
Appendix D (Informative) Nested PCR Product Electrophoresis and Fluorescence PCR Amplification Curve 14
Appendix E (Informative) Preparation and Identification of Recombinant P2 Protein of Glaseria parasuis 15
Appendix F (Informative) Preparation and Identification of Antibody Positive Control (Positive Serum) and Negative Control (Negative Serum) 16
Appendix G (Normative) Preparation of indirect ELISA test solution 17
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 34750-2017 "Detection method for Haemophilus parasuis". Compared with GB/T 34750-2017, except for the structural adjustment
In addition to the integration and editorial changes, the main technical changes are as follows.
--- Changed the scope (see Chapter 1, Chapter 1 of the.2017 edition);
--- Added clinical diagnosis (see Chapter 5);
---Changed sample collection, processing and storage (see Chapter 6, 6.1 of the.2017 edition);
--- Changed the result judgment of bacterial isolation and identification (see 7.8, 6.6.7 of the.2017 edition);
--- Changed the preparation of sample DNA (see 8.3, 7.2 of the.2017 edition);
---Indirect ELISA was added (see Chapter 10);
--- Added comprehensive judgment (see Chapter 11).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181).
This document was drafted by. Henan Provincial Animal Disease Prevention and Control Center, Henan Provincial Livestock Product Quality Monitoring and Inspection Center, China Animal Health
and Epidemiology Center, Institute of Animal Husbandry and Veterinary Medicine, Henan Academy of Agricultural Sciences.
The main drafters of this document are. Yan Ruoqian, Ban Fuguo, Xie Caihua, Wu Zhiming, Su Hong, Cheng Guo, Ma Zhenyuan, Wang Shujuan, Zhao Mingjun, Xu Yindi,
Guo Yupei, Yang Haibo, Wang Cui, Liu Ying, Zhao Xueli, Fang Xianzhen, Fang Daxue, Song Dan, Zhao Meixue, Chai Mao, Liu Min, Zhang Liping, Liu Lijie, Li Jiao,
Zhao Shengjie and Ran Xiaolong.
The previous versions of this document and the documents it replaces are as follows.
---First published in.2017 as GB/T 34750-2017.
---This is the first revision.
Glasser's disease diagnosis technology
1 Scope
This document describes the clinical diagnosis of Glaser's disease, sample collection, processing and storage, bacterial isolation and identification, nested PCR detection, real-time
Laboratory diagnostic methods such as fluorescent PCR detection and indirect ELISA.
This document applies to the diagnosis and monitoring of Glaser's disease.
2 Normative references
This document has no normative references.
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
bp. base pair
Ct value. cycle threshold
IgG. immunoglobulin Gs
Taq enzyme. Taq DNA polymerase
TSB. tryptic soy broth
5 Clinical diagnosis
5.1 Epidemiology
Glasser's disease is a bacterial infectious disease caused by Glasseria parasuis. Glasseria parasuis only infects pigs. Sick pigs and carrier pigs are
The main source of infection of the disease. Pigs of different ages, genders and breeds are susceptible, and the disease mainly occurs in pigs before and after weaning and in the nursery stage.
It can occur in all seasons, especially in early spring and late autumn. It mainly infects the respiratory tract and can be divided into acute infection and chronic infection.
Mainly sexually infected.
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