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GB/T 34745-2017 English PDF

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GB/T 34745-2017: Porcine circovirus type 2 -- Method of detecting virus by SYBR Green Ⅰ real-time PCR
Status: Valid
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GB/T 34745-2017English179 Add to Cart 3 days [Need to translate] Porcine circovirus type 2 -- Method of detecting virus by SYBR Green Ⅰ real-time PCR Valid GB/T 34745-2017

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Basic data

Standard ID GB/T 34745-2017 (GB/T34745-2017)
Description (Translated English) Porcine circovirus type 2 -- Method of detecting virus by SYBR Green �� real-time PCR
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 9,974
Date of Issue 2017-11-01
Date of Implementation 2018-05-01
Regulation (derived from) National Standard Announcement 2017 No. 29
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34745-2017: Porcine circovirus type 2 -- Method of detecting virus by SYBR Green Ⅰ real-time PCR


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Porcine circovirus type 2-Method of detecting virus by SYBR Green I real-time PCR ICS 11.220 B41 National Standards of People's Republic of China Porcine circovirus type 2 virus SYBRGreen I real-time Fluorescent Quantitative PCR Detection Method Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard drafting unit. Henan Agricultural University, China Animal Health and Epidemiology Center. The main drafters of this standard. Wei Zhan Yong, Zheng Lanlan, Shao Satellite, Hu Hui, Chen Hongying, Wang Yabin, Wang Xuebing, Cui Baoan, Kouanan. Porcine circovirus type 2 virus SYBRGreen I real-time Fluorescent Quantitative PCR Detection Method

1 Scope

This standard specifies the SYBRGreen Ⅰ real-time fluorescent quantitative PCR detection of porcine circovirus type 2 technical requirements. This standard for the detection of porcine circovirus type 2 nucleic acid for rapid detection of porcine circovirus type 2 in clinical samples.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this document. Ct value. CycleThreshold DNA. Deoxyribonucleic Acid EDTA. Ethylenediamine tetraacetic acid (EthylenediaminetetraaceticAcid) PBS. Phosphate buffer solution (PhosphateBufferSaline) PCR. Polymerase Chain Reaction (Polymerase Chain Reaction) PCV2. Porcine Circovirus Type 2 PMWS. Postweaning Multisystemic Wasting Syndrome Taq enzyme Taq DNA polymerase (Taq DNA Polymerase) tm value. Melting temperature (MeltingTemperature)

4 reagent

Unless otherwise specified, only analytical reagents are used. All reagents were treated with RNase-free containers (high pressure after water treatment with DEPC Sterilization) sub-packaging. 4.1 Proteinase K (preparation see Appendix A) - 20 ℃ preservation. 4.2 lysis buffer (preparation see Appendix A) .4 ℃ preservation. 4.3 guanidine isothiocyanate lysis solution (preparation see Appendix A) .4 ℃ preservation. 4.4 Balance phenol (preparation see Appendix A) .4 ℃ preservation. 4.5 phenol - chloroform - isoamyl alcohol (25.24. 1 mixture (preparation see Appendix A) .4 ℃ preservation. 4.6 chloroform - isoamyl alcohol (24. 1) (preparation see Appendix A) .4 ℃ preservation. 4.7 Chloroform. Store at room temperature. 4.8 isopropanol. Store at room temperature. 4.9 70% ethanol Prepared with freshly opened absolute ethanol and sterile deionized water (complying with GB/T 6682), pre-cooled at 20 ° C.

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