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GB/T 34740-2017 English PDF

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GB/T 34740-2017: Diagnostic methods for animal rabies diagnosis with direct immunofluorescence assay
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PDF similar to GB/T 34740-2017


Standard similar to GB/T 34740-2017

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Basic data

Standard ID GB/T 34740-2017 (GB/T34740-2017)
Description (Translated English) Diagnostic methods for animal rabies diagnosis with direct immunofluorescence assay
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 8,862
Date of Issue 2017-11-01
Date of Implementation 2018-05-01
Regulation (derived from) National Standard Announcement 2017 No. 29
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34740-2017: Diagnostic methods for animal rabies diagnosis with direct immunofluorescence assay


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Diagnostic methods for animal rabies diagnosis with direct immunofluorescence assay ICS 11.220 B41 National Standards of People's Republic of China Animal rabies immunofluorescence diagnostic method Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard reference the World Animal Health Organization (OIE).2013 edition of terrestrial animal health manual 2.1.13 rabies related content. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard was drafted by the PLA Military Academy of Military Medical Veterinary Research Institute. Drafters of this standard. Hu Rongliang, Zhang Shoufeng, Liu Ye, Zhang Fei, with the sea. Animal rabies immunofluorescence diagnostic method

1 Scope

This standard specifies the materials and reagents, equipment and equipment, methods of protection, animal brain Sample collection, transportation and preservation, experimental operation and microscopic observation and determination of results. This standard applies to animal rabies etiology diagnosis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. Laboratory use specifications and test methods for analysis GB/T 6682-2008

3 Abbreviations

The following abbreviations apply to this document. DFA. Direct Immunofluorescence Assay FITC Fluorescein Isothiocyanate PBS. Phosphate-Buffered Saline

4 materials and reagents

Unless otherwise specified, only analytical reagents are used. 4.1 water, GB/T 6682-2008, two water. 4.2 rabies positive control brain tissue (see A.1). 4.3 Rabies negative control brain tissue (see A.2). 4.4 FITC-labeled anti-rabies virus antibody (commercial fluorescent antibody) should be anti-rabies virus nucleoprotein monoclonal antibody or Prepared from polyclonal antibodies, diluted with PBS (0.01mol/L, pH7.4, see B.1) into a working solution, now with the current service. 4.5 1% Evans blue solution (see B.2). 4.6 Stationary fluid (80% acetone solution, see B.3). Warning --- acetone flammable, harmful to health 4.7 PBS (0.01 mol/L, pH 7.4, see B.1). 4.8 80% alkaline buffered glycerol solution (see B.4).

5 Equipment and equipment

5.1 necropsy sampling and protective equipment Scalpel, scissors, forceps, tweezers, latex gloves, goggles.

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