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GB/T 28981-2012: Detection and identification of odontoglossum ringspot virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 28981-2012 | English | 279 |
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Detection and identification of odontoglossum ringspot virus
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GB/T 28981-2012
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Basic data | Standard ID | GB/T 28981-2012 (GB/T28981-2012) | | Description (Translated English) | Detection and identification of odontoglossum ringspot virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 12,111 | | Regulation (derived from) | National Standards Bulletin No. 41 of 2012 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the Odontoglossum ringspot virus serology and molecular biological characteristics of the detection method. This standard applies to seeds, bulbs, flowers and seeds and other plant material Odontoglossum ringspot virus detection, b | GB/T 28981-2012: Detection and identification of odontoglossum ringspot virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of odontoglossum ringspot virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Odontoglossum ringspot virus quarantine and identification methods
Issued on. 2012-12-31
2013-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China, Ningbo Entry-Exit Inspection and Quarantine Shanghai People's Republic of China
Bureau, Chinese Academy of Inspection and Quarantine, People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters of this standard. Wen Weigang, Chen Xianfeng, Yang Cuiyun, Guo Lixin, Lu Jie, Zhang Yongjiang, Zhang Mingzhe, Xu Ying, Cuijun Xia, Zhang Huili.
Odontoglossum ringspot virus quarantine and identification methods
1 Scope
This standard specifies the method for detecting biological characteristics of spot serological and molecular Odontoglossum ring.
This standard is applicable to the detection of seeds, bulbs, flowers and seeds and other plant material Tooth ringspot virus, but also for aphids and other communication media
Medium detected the virus.
2 Odontoglossum ringspot virus basic information
Chinese name. Odontoglossum ringspot virus
Name. odontoglossumringspotvirus
Abbreviation. ORSV
Tobacco mosaic virus belongs to the genus (Tobamovirus), Odontoglossum ringspot virus For additional information, see Appendix A.
3 PRINCIPLE OF THE METHOD
Based on the use of antigen-antibody reaction of the double-antibody sandwich enzyme-linked immunosorbent assay (Doubleantibodysandwichenzyme-linked
immunosorbentassay, DAS-ELISA), reverse transcription polymerase chain reaction in vitro DNA synthesis technology (Reverse
transcriptionpolymerasechainreaction, RT-PCR), real-time fluorescence reverse transcription-polymerase chain reaction (real-time
fluorescentRT-PCR) detection and identification.
4 equipment, appliances and reagents
4.1 Equipment
PCR, real-time PCR instrument, clean benches, electronic balance (the amount of sense. 0.001g), electrophoresis, electrophoresis tank, gel imaging system,
High-speed refrigerated centrifuge, ultra-low temperature freezer (-80 ℃), autoclaves, small-scale food processing machines, microwave ovens, microplate reader.
4.2 Appliances
Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and the corresponding RNase-free tip, no
RNase centrifuge tubes, PCR tubes and mortar and the like.
4.3 Reagents
Reagents and buffers see Appendices B and C.
5 Detection
5.1 Sample Preparation
Take 0.5g ~ 10g seed, bulb scales or deformity, discoloration symptoms leaves and other plant tissues, in a mortar or small food processor
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