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US$509.00 · In stock Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 28978-2012: Detection and identification of Clavibacter michiganensis subsp. sepedonicus Status: Valid
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Detection and identification of Clavibacter michiganensis subsp. sepedonicus
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GB/T 28978-2012
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Basic data | Standard ID | GB/T 28978-2012 (GB/T28978-2012) | | Description (Translated English) | Detection and identification of Clavibacter michiganensis subsp. sepedonicus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 22,230 | | Quoted Standard | SN/T 1135.5-2007 | | Regulation (derived from) | National Standards Bulletin No. 41 of 2012 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies based on physiological and biochemical characteristics, pathogenicity characteristics, serology, molecular biological characteristics of the potato ring rot pathogen detection and identification methods. This standard applies to po |
GB/T 28978-2012: Detection and identification of Clavibacter michiganensis subsp. sepedonicus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Clavibacter michiganensis subsp.sepedonicus
ICS 65.020.01
B16
National Standards of People's Republic of China
Potato Ring Rot quarantine and identification methods
Issued on. 2012-12-31
2013-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Bureau Technology Center, People's Republic of China Fujian
Inspection & Quarantine Technology Center, People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters of this standard. Liao Furong, Lin Shiming, Wu Yuan, Shen Jianguo, Wu Zhiyi, Chen Qing, Zhang Mingzhe, Chen Yun, Peng Ying Huang.
Potato Ring Rot quarantine and identification methods
1 Scope
This standard specifies based on physiological and biochemical characteristics of potato ring rot pathogen detection and pathogenic characteristics, serological, molecular biological characteristics
Identification methods.
This standard applies to Potato Ring Rot in potato tubers, detection and identification of plants on.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1135.5-2007 potato ring rot pathogen identification of quarantine
3 Basic information of Potato Ring Rot
Chinese Name. Michigan Corynebacterium ring rot subspecies
Common name. Potato Ring Rot
Latin name. Clavibactermichiganensissubsp.sepedonicus
Belonging to Microbacterium Branch (Microbacteriaceae), Rod genus (Clavibacter) members.
Potato Ring Rot (hereinafter referred Cms) caused by potato ring rot, can be transported over long distances through the diseased seed, also reported
By tomato seed dispersal, additional information about Cms see Appendix A.
4 principle of the method
Potato Ring Rot has a unique cultural characteristics and physiological and biochemical, physiological and biochemical characteristics of the bacteria, pathogenic characteristics, and
Serological, molecular biological characteristics is the main basis for the development of this standard.
5 Key Equipment
The standard detection methods identified mainly with the following equipment.
Trace juicer, microplate reader, washer, microbalance (sense of volume. 0.001g), bio-incubator, fluorescence microscopy, PCR, fluorescence
PCR, electrophoresis, horizontal electrophoresis tank gel imager, high-speed refrigerated centrifuge desktop, BIOLOG automated microbial identification system, turbidity
Meter, thermostat water bath or incubator, pH meter, a variety of range adjustable pipette (1000μL, 200μL, 100μL, 20μL, 10μL, 2μL).
6 Detection and Identification
6.1 Symptom Check
After the potato ring rot pathogen infection in potato plants in the potato tubers, plants can produce symptoms (see Appendix A), by detecting the symptoms
Preliminary investigation can determine whether the disease is caused by Cms.
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