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GB/T 28073-2011 English PDF

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GB/T 28073-2011: Detection and identification of arabis mosaic virus
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GB/T 28073-2011English299 Add to Cart 3 days [Need to translate] Detection and identification of arabis mosaic virus Valid GB/T 28073-2011

PDF similar to GB/T 28073-2011


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Basic data

Standard ID GB/T 28073-2011 (GB/T28073-2011)
Description (Translated English) Detection and identification of arabis mosaic virus
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 13,116
Date of Issue 2011-12-30
Date of Implementation 2012-06-01
Regulation (derived from) Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the Arabis mosaic virus serology and molecular biology methods for detection and identification. This standard applies to plant seeds, scaly bulbs and plants and their products such as tissue culture South mustard mosaic virus detection and identification.

GB/T 28073-2011: Detection and identification of arabis mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of arabis mosaic virus ICS 65.020.01 B16 National Standards of People's Republic of China Arabis mosaic virus quarantine and identification methods Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Shanghai People's Republic of China Bureau, Chinese Academy of Inspection and Quarantine, People's Republic of China Fujian Exit Inspection and Quarantine. The main drafters of this standard. Liao Furong, in Tsui, Zhang Yongjiang, Chen Yun, Lin Shiming, Chen Qing, Huang Peng Ying, Shen Jianguo, Wu Yuan. Arabis mosaic virus quarantine and identification methods

1 Scope

This standard specifies the method for detection and identification of Arabis mosaic virus serology and molecular biology. This standard applies to plant seeds, the detection and identification of scales bulbs, seedlings and tissue culture and other plants and their products SOUTH Arabis mosaic virus.

2 Arabis mosaic virus Basic Information

Chinese name. Arabis mosaic virus. English name. arabismosaicvirus. Synonyms. arabismosaicnepovirus; hopnettleheadvirus (hop nettle disease virus); rhubarbmosaicvirus (large Yellow mosaic virus); raspberryyelowdwarfvirus (Rubus yellow dwarf virus); ashringandlinepatternviurs (ash tree Ring-like virus); rhabarber-mosaik-virus (rhubarb mosaic virus); forsythiayelownetvirus (forsythia yellow mesh virus); jasmineyelowblotchvirus (Jasmine macular virus). English abbreviation. ArMV. Genus cowpea mosaic virus family Comoviridae, nematodes pass polyhedron virus genus Nepovirus. For additional information, see Appendix A. Arabis mosaic virus

3 PRINCIPLE OF THE METHOD

The use of double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on antigen-antibody reaction, reverse transcription and in vitro DNA amplification technology Surgery reverse transcription polymerase chain reaction (RT-PCR) detection and identification.

4 Main equipment

The standard detection methods identified mainly with the following equipment. Trace juicer, microplate reader, washer, microbalance (sense of volume. 0.001g), PCR, fluorescence PCR, electrophoresis, horizontal electrophoresis Groove, gel imager, desktop high-speed refrigerated centrifuge, water bath, pH meter, and a variety of ranges adjustable pipette (1000μL, 200μL, 100μL, 20μL, 10μL, 2μL).

5 Detection and Identification

5.1 DAS-ELISA detection The sample is thoroughly 0.5g ~ 1.0g grinding or milling in liquid nitrogen, 1.10 and added to the sample extraction buffer, transferred to 5mL Centrifuge tube, 8000r/min centrifugal 5min, supernatant as DAS-ELISA detection extract. Setting a negative control, the positive control and blank control, negative control and the type of material (such as. seed or blade) should try and detected Consistent sample type. Specific operation in Appendix B. Note. The extract used in 3h, otherwise the stored at 4 ℃.

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