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GB/T 28064-2011: Detection and identification of broad bean stain virus
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| GB/T 28064-2011 | English | 259 |
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Detection and identification of broad bean stain virus
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GB/T 28064-2011
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Basic data | Standard ID | GB/T 28064-2011 (GB/T28064-2011) | | Description (Translated English) | Detection and identification of broad bean stain virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 11,184 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-06-01 | | Quoted Standard | SY/T 2122 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the broad bean stain virus serology and molecular detection methods. This standard applies to broad beans (Vicia faba), lentil (Lens culinaris), peas (Pisum sativum) and common bean (Phaseolus vulgaris) seeds carrying the virus quarantine and identification of broad bean stain. | GB/T 28064-2011: Detection and identification of broad bean stain virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of broad bean stain virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Quarantine and identification of broad bean stain virus
Issued on. 2011-12-30
2012-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, the People's Republic of China
And country SZCIQ.
The main drafters of this standard. Chen Yun, Chen Qing, Lin Shiming, Zheng Yun, Zhao Wenjun, Li Yinong, Liao Furong, Yufang Ping, water Zhu Fang, Chen Chi-nan.
Quarantine and identification of broad bean stain virus
1 Scope
This standard specifies the serological and molecular detection methods bean stain virus.
This standard applies to beans Viciafaba, lentils Lensculinaris, peas and beans Phaseolus Pisumsativum
vulgaris seed carrying quarantine and identification of broad bean stain virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 Basic information of broad bean stain virus
Chinese name. broad bean stain virus.
Name. broadbeanstainvirus.
Abbreviation. BBSV.
Genus cowpea mosaic virus family Comoviridae; comovirus Comovirus.
Diseased plants pollinated by pollen can be spread to healthy plants seed virus; distance via seed dispersal, seed-borne hosts have beans (seed transmission rate of 4%
16%), peas (20%) and lentils (0.2% to 32.4%). BBSV mechanical inoculation spread easily, and through the beetle mass poisoning, long beak like beans
A Apionvorax mass poisoning is the main mediator.
For additional information, see Appendix A. bean stain virus
4 principle of the method
Mainly based on serological characteristics and genomic features BBSV were identified, biological assays for the identification of the auxiliary means.
5 equipment, facilities and reagents
5.1 Instruments and facilities
Enzyme-linked detector, electronic balance (inductance 0.0001g), qualitative PCR, electrophoresis, electrophoresis tank, UV transmission meter, water bath, low
Refrigerator temperature, ordinary refrigerator, centrifuge; micropipette (2.5μL, 10μL, 20μL, 100μL, 200μL, 1000μL); ELISA plate, RESEARCH
Beryllium; pest control greenhouse.
5.2 Reagents
Enzyme-linked immunosorbent assay reagent (see Appendix B), RT-PCR detection reagent (see Appendix C).
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