| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 28063-2011 | English | 150 |
Add to Cart
|
0--9 seconds. Auto-delivery
|
Detection and identification of Bean pod mottle virus
| Valid |
GB/T 28063-2011
|
PDF similar to GB/T 28063-2011
Basic data | Standard ID | GB/T 28063-2011 (GB/T28063-2011) | | Description (Translated English) | Detection and identification of Bean pod mottle virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,153 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-06-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the bean pod mottle virus serology and molecular biology procedures and methods of detection and identification. This standard applies may carry the virus legume seeds, seedlings and other plant propagating material detection and identification, but also to spread the virus detection and identification of insects mediator. | GB/T 28063-2011: Detection and identification of Bean pod mottle virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Bean pod mottle virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Bean pod mottle virus, quarantine and identification methods
Issued on. 2011-12-30
2012-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Fujian People's Republic of China,
People's Republic of China, Shenzhen CIQ.
The main drafters of this standard. Liao Furong, Shen Jianguo, Zheng Yun, Chen Qing, Lin Shiming, Chen Yun, Huang Peng Ying, Wu Yuan.
Bean pod mottle virus, quarantine and identification methods
1 Scope
This standard specifies the method for detection and identification of bean pod mottle virus serology and molecular biology.
This standard applies may carry the virus detection and identification of legume seeds, seedlings and other plant propagation material, but also to spread
The mediator virus detection and identification of insects.
2 Basic information of bean pod mottle virus
Chinese name. bean pod mottle virus.
English name. beanpodmottlevirus.
Synonyms. beanpodmottlecomovirus (bean pod mottle virus), podmottlevirus (pod mottle virus), desmodium
virus (loosestrife virus).
English abbreviation. BPMV.
Genus cowpea mosaic virus family Comoviridae, cowpea mosaic virus genus Comovirus.
Bean clip Mottle Virus For additional information, see Appendix A.
3 PRINCIPLE OF THE METHOD
The use of double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on antigen-antibody reaction, reverse transcription and in vitro DNA amplification technology
Surgery reverse transcription polymerase chain reaction (RT-PCR) detection and identification.
4 Main equipment
The standard detection methods identified mainly with the following equipment.
Trace juicer, microplate reader, washer, microbalance (sense of volume. 0.001g), PCR, fluorescence PCR, electrophoresis, horizontal electrophoresis
Groove, gel imager, desktop high-speed refrigerated centrifuge, water bath, pH meter range and a variety of adjustable pipette (1000μL, 200μL,
100μL, 20μL, 2μL).
Preparation of the test sample 5
Preparation of 5.1 DAS-ELISA and IC-RT-PCR for detecting samples
Inspection of each batch of samples symptom checker, prefers the seeds have brown or black mottle symptoms (from the hilum start), or choose
Having a blade symptoms mottled, wrinkled, chlorosis and so on.
Seed samples. Weigh 0.5g ~ 1.0g seed coat as the test sample, is thoroughly ground in liquid nitrogen and warmed at 1.10 after added
Sample extraction buffer and grinding continued.
Leaf samples. Weigh 0.5g ~ 1.0g leaves as a test sample, grind in a mortar or grinding in liquid nitrogen, 1.10
Sample extraction buffer was added and grinding continued.
The ground sample was transferred to 5mL centrifuge tube, 8000r/min centrifugal 5min, supernatant as DAS-ELISA (see Annex
Record B) and IC-RT-PCR (see Appendix C) detection extract.
Note. The extract used in 3h, otherwise the stored at 4 ℃.
|