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GB/T 20190-2025 English PDF

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GB/T 20190-2025: Determination of bovine, ovine and goat-derived materials in feeds
Status: Valid

GB/T 20190: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB/T 20190-2025English309 Add to Cart 3 days [Need to translate] Determination of bovine, ovine and goat-derived materials in feeds Valid GB/T 20190-2025
GB/T 20190-2006English140 Add to Cart 0--9 seconds. Auto-delivery Detection of bovine, sheep and goat-derived material in feeds - Qualitative polymerase chain reaction (PCR) method Valid GB/T 20190-2006

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Basic data

Standard ID GB/T 20190-2025 (GB/T20190-2025)
Description (Translated English) Determination of bovine, ovine and goat-derived materials in feeds
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B46
Classification of International Standard 65.120
Word Count Estimation 15,132
Date of Issue 2025-06-30
Date of Implementation 2026-01-01
Older Standard (superseded by this standard) GB/T 20190-2006
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 20190-2025: Determination of bovine, ovine and goat-derived materials in feeds

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT20190-2025
ICS 65.120 CCSB46 National Standard of the People's Republic of China Replaces GB/T 20190-2006 Determination of ingredients of cattle, sheep and goat origin in feed Released on June 30, 2025 Implementation on January 1, 2026 State Administration for Market Regulation The National Standardization Administration issued

Preface

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 20190-2006 "Qualitative detection of cattle and sheep-derived ingredients in feeds - Qualitative polymerase chain reaction (PCR) Compared with GB/T 20190-2006, in addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope of application and detection limit have been changed (see Chapter 1, Chapter 1 of the.2006 edition); b) Added a chapter on “Abbreviations” (see Chapter 4); c) Added a chapter on “Real-time Fluorescence Polymerase Chain Reaction” (see Chapter 5); d) The principles, reagents and materials, DNA extraction methods, PCR detection methods, and result interpretation of the polymerase chain reaction (PCR) method have been changed. Definition and description of quality control (see Chapter 6, Chapters 3, 4, 6 and 7 of the.2006 edition); e) Added a chapter on “Measures for the Prevention and Control of Laboratory Contamination” (see Chapter 7); f) Added a chapter on “Hazardous Waste Disposal” (see Chapter 8); g) Added internal reference genes and target gene sequences for PCR detection in cattle, sheep, and goats (see Appendix B). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Shanghai Customs Animal, Plant and Food Inspection and Quarantine Technical Center and New Hope Liuhe Co., Ltd. The main drafters of this document are. Pan Liangwen, Cai Yicun, Zhang Yuqiu, Han Wei, Yang Qing, Zhang Chen, Shao Bingyu, Xu Zhenjian, Ning Xue, and Lü You. The previous versions of this document and the documents it replaces are as follows. ---First published in.2006 as GB/T 20190-2006; ---This is the first revision. Determination of ingredients of cattle, sheep and goat origin in feed

1 Scope

This document describes the detection of bovine, ovine, and caprine origin ingredients in feed by real-time fluorescent polymerase chain reaction and polymerase chain reaction. Test method. This document applies to compound feeds, concentrated feeds, concentrate supplements, feed ingredients, compound premix feeds and mixed feed additives. Detection of ingredients of bovine, ovine, and caprine origin. The detection limit for this document is 0.1%.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 19495.3-2004 Methods for extraction and purification of nucleic acids for detection of genetically modified products GB/T 20195 Preparation of animal feed samples GB/T 27403-2008 Laboratory quality control specification for food molecular biology testing GB/T 35918-2018 Detection of animal origin in animal products—Gene barcoding technology—Sanger sequencing method

3 Terms and Definitions

There are no terms or definitions that require definition in this document.

4 Abbreviations

The following abbreviations apply to this document. bp. base pair Ct. cycle threshold 5 Real-time fluorescent polymerase chain reaction 5.1 Principle Primers and probes were designed based on the specific sequences of bovine β-actin gene, sheep prolactin receptor gene and goat chromosome 9 gene.

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