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GB/T 14926.21-2008: Laboratory animal -- Method for examination of rabbit hemorrhagic disease virus (RHDV)
Status: Valid GB/T 14926.21: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 14926.21-2008 | English | 169 |
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Laboratory animal -- Method for examination of rabbit hemorrhagic disease virus (RHDV)
| Valid |
GB/T 14926.21-2008
|
| GB/T 14926.21-2001 | English | 199 |
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Laboratory animal -- Method for examination of rabbit hemorrhagic disease virus (RHDV)
| Obsolete |
GB/T 14926.21-2001
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| GB/T 14926.21-1994 | English | 199 |
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Method for examination of rabbit hemorrhagic disease virus in laboratory animal
| Obsolete |
GB/T 14926.21-1994
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PDF similar to GB/T 14926.21-2008
Basic data | Standard ID | GB/T 14926.21-2008 (GB/T14926.21-2008) | | Description (Translated English) | Laboratory animal -- Method for examination of rabbit hemorrhagic disease virus (RHDV) | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B44 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 7,776 | | Date of Issue | 2008-12-10 | | Date of Implementation | 2009-03-01 | | Older Standard (superseded by this standard) | GB/T 14926.21-2001 | | Quoted Standard | GB/T 14926.53; GB/T 14926.54 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.19 (Total No.132) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the rabbit hemorrhagic disease virus (RHDV) detection method. This section applies to rabbit RHDV antigen, detection of antibodies and viral nucleic acids. | GB/T 14926.21-2008: Laboratory animal -- Method for examination of rabbit hemorrhagic disease virus (RHDV)
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Laboratory animal. Method for examination of rabbit hemorrhagic disease virus (RHDV)
ICS 65.020.30
B44
National Standards of People's Republic of China
Replacing GB/T 14926.21-2001
Animal rabbit hemorrhagic disease virus detection method
Posted 2008-12-10
2009-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 14926 "animal" part of a total of 54, different microbes and virus detection methods.
Since the implementation of this section instead of GB/T 14926.21-2001 "animal rabbit hemorrhagic disease virus detection methods."
This part of the main technical differences compared with the GB/T 14926.21-2001 as follows.
a) increasing the rabbit hemorrhagic disease virus nucleic acid (RHDV) nucleic acid detection methods;
b) determining serum antibodies in rabbits immunized RHDV vaccine eligibility criteria.
This part of the National Committee for Standardization of animal experiments and centralized.
This section drafted by. National Standardization Technical Committee of experimental animals.
The main drafters of this part. He contention, Rui Fu, TIAN Ke-gong.
This part of the standard replaces the previous editions are.
--- GB/T 14926.21-1994, GB/T 14926.21-2001.
Animal rabbit hemorrhagic disease virus detection method
1 Scope
GB/T 14926 provisions of this part of the rabbit hemorrhagic disease virus (RHDV) detection method.
This section applies to detect RHDV rabbit antigens, antibodies and viral nucleic acids.
2 Normative references
The following documents contain provisions which, through reference GB/T 14926 in this section constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
GB/T 14926.53 animals hemagglutination
GB/T 14926.54 animal hemagglutination inhibition test
Principle 3
Hemagglutination test (HA). Under certain conditions, RHDV could agglutinate human "O" type red blood cells, resulting in visible agglutination. According to this
Characteristic detecting presence or absence of RHDV rabbit liver tissue antigens.
Hemagglutination inhibition assay (HAI). Under certain conditions, RHDV agglutination people "O" type red blood cell capacity can be inhibited by specific antibodies.
Have RHDV antibody according to the characteristic detection rabbit serum.
Nucleic acid testing. According RHDVVP60 gene sequence conserved characteristics, specific primers, amplified fragment detected in rabbit tissues
The RHDV nucleic acid.
4 Reagents and Equipment
4.1 Reagents
4.1.1 hemagglutinin
Artificial or natural infection pathogenesis of rabbit liver tissue, by grinding a 10% suspension, 3000r/min centrifugal 10min obtained
Serum.
4.1.2 positive serum
RHDV antigen or naturally infected with RHDV rabbit serum recovered.
4.1.3 negative serum
No RHDV infection without immune rabbit serum.
4.1.4 people "O" type red blood cells.
4.1.5 and buffer solution
4.1.5.1 PBS (pH7.4)
NaCl 8g
KCl 0.2g
Na2HPO4 · 12H2O 2.83g
KH2PO4 0.2g
Deionized water 1000mL
4.1.5.2 TRIzolReagent.
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