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GB/T 14926.56-2008: Laboratory animal -- Method for examination of rabies virus
Status: Valid GB/T 14926.56: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 14926.56-2008 | English | 139 |
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Laboratory animal -- Method for examination of rabies virus
| Valid |
GB/T 14926.56-2008
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| GB/T 14926.56-2001 | English | 199 |
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Laboratory animal -- Method for examination of Rabies virus (RV)
| Obsolete |
GB/T 14926.56-2001
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PDF similar to GB/T 14926.56-2008
Basic data | Standard ID | GB/T 14926.56-2008 (GB/T14926.56-2008) | | Description (Translated English) | Laboratory animal -- Method for examination of rabies virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B44 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 6,637 | | Date of Issue | 2008-12-10 | | Date of Implementation | 2009-03-01 | | Older Standard (superseded by this standard) | GB/T 14926.56-2001 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.19 (Total No.132) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the rabies virus (RV) detection methods, reagents. This section applies to dogs RV detection. | GB/T 14926.56-2008: Laboratory animal -- Method for examination of rabies virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Laboratory animal.Method for examination of rabies virus
ICS 65.020.30
B44
National Standards of People's Republic of China
Replacing GB/T 14926.56-2001
Experimental animal rabies virus detection method
Posted 2008-12-10
2009-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 14926 "animal" part of a total of 54, different microbes and virus detection methods.
Since the implementation of this section instead of GB/T 14926.56-2001 "animal rabies virus detection methods."
This part of the main technical differences compared with the GB/T 14926.56-2001 as follows.
a) delete the original standard "2 reference standard" content;
b) the original standard "3 principles" in everything the words "on the pre-coated microplate purified rabies virus antigen in the immune response
Performed on a solid support. When the subject serum rabies virus antibodies are present, then the pore walls with an antigen to form an antigen - antibody
Complex, and then with anti-dog IgG enzyme conjugate reaction, the final product was measured by enzyme catalyzed substrate after qualitative
Detection ";
c) Remove all original standard hemagglutination inhibition test on reagents, methods and results of determination;
d) all of the content of the original standard ELISA test on the changes, including reagents, methods and results criteria, etc.;
e) of the original standard "6 results found" added "immune antibody level pass rate of 6.3 based dog groups. the passing rate of antibody =
(Subject animal antibody positive/is the total number of seized animals) × 100%. Ground-level pass rate of antibody dog population reached 70%
The penalty for the dog population qualified immunity "content.
This part of the National Standardization Technical Committee of experimental animals and focal points.
This part of the National Standardization Technical Committee responsible for drafting the experimental animals.
The main drafters of this section. TIAN Ke-gong, Chen Xi Zhao, Cao, Wang Chuanbin, Hexiu Min, Tang Hanwen.
This part of the standard replaces the previous editions are.
--- GB/T 14926.56-2001.
Experimental animal rabies virus detection method
1 Scope
GB/T 14926 provisions of this part of the rabies virus (RV) of the detection methods, reagents and the like.
This section applies to RV detection dogs.
Principle 2
On the plate pre-coated with purified rabies virus antigen, the immune response on a solid carrier. When the subject serum rabies disease
When the presence of an antibody drug, the wall of the hole with an antigen to form an antigen - antibody complex, and then reacted with anti-dog IgG enzyme markers, and finally by determining enzyme
The product substrates of catalytic after qualitative testing.
3 Reagents and equipment
3.1 Reagents
Canine rabies virus IgG antibody test kit (qualitative), comprising.
a) pre-coated microplate rabies virus antigen;
b) rabies virus IgG enzyme label;
c) rabies virus IgG positive control;
d) critical rabies virus IgG control;
e) rabies virus IgG negative control;
f) color reagent A. = sodium hydrogen phosphate - citrate buffer (0.05mol/L, pH5.0) 1000mL, 30% hydrogen peroxide H2O2
(MW34.0) 3.5mL;
g) color reagent B. citric acid 1.052g, disodium ethylenediaminetetraacetate (EDTA-Na2) 0.186g, tetramethylbenzidine (TMB)
0.25g, dimethylsulfoxide (DMSO) 8.0mL, purified water 992mL;
h) Stop Solution. 98% sulfuric acid 27.8mL, purified water 972.2mL;
i) 10-fold concentrated washing solution. PBS (0.1mol/LpH7.2 ~ 7.4) 995mL, Tween -20,5mL;
j) Sample diluent. borate buffer (0.01mol/LpH8.0) 956.0mL, glycerol (MW92.09) 40.0mL, casein
(Casein) 10% thimerosal solution 2.0mL, Tween -20,1.0mL1% phenol red solution, 1.0mL.
Reagent should be brought to room temperature before use (18 ℃ ~ 25 ℃).
3.2 Equipment
Equipment includes.
a) precision pipette 10μL ~ 100μL;
b) a disposable pipette tip;
c) oscillator;
d) microplate reader (including 450nm wavelength filter);
e) 37 ℃ incubator;
f) distilled or deionized water;
g) 100mL graduated cylinder;
h) centrifuge;
i) absorbent paper.
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