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GB/T 14926.47-2008 English PDF

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GB/T 14926.47-2008: Laboratory animal -- Method for examination of shigella spp
Status: Valid

GB/T 14926.47: Evolution and historical versions

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 14926.47-2008	English	139	Add to Cart	3 days [Need to translate]	Laboratory animal -- Method for examination of shigella spp	Valid	GB/T 14926.47-2008
GB/T 14926.47-2001	English	199	Add to Cart	2 days [Need to translate]	Laboratory animal -- Method for examination of Shigella sp	Obsolete	GB/T 14926.47-2001

PDF similar to GB/T 14926.47-2008

Standard similar to GB/T 14926.47-2008

GB/T 43051 GB/T 39760 GB/T 35823 GB/T 14926.57 GB/T 14926.58 GB/T 14926.56

Basic data

Standard ID	GB/T 14926.47-2008 (GB/T14926.47-2008)
Description (Translated English)	Laboratory animal -- Method for examination of shigella spp
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	B44
Classification of International Standard	65.020.30
Word Count Estimation	6,647
Date of Issue	2008-12-03
Date of Implementation	2009-03-01
Older Standard (superseded by this standard)	GB/T 14926.47-2001
Quoted Standard	GB/T 14926.42; GB/T 14926.43
Regulation (derived from)	National Standard Approval Announcement 2008 No.19 (Total No.132)
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary	This standard specifies a method for detecting animals Shigella. This section applies to detect the monkey Shigella.

GB/T 14926.47-2008: Laboratory animal -- Method for examination of shigella spp

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.020.30 B44 National Standards of People's Republic of China Replacing GB/T 14926.47-2001 Laboratory animal Shigella detection method 2008-12-03 released 2009-03-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

GB/T 14926 "experimental animals" a total of 54 parts, for different microbial and virus detection technology. This part of the implementation of the date of replacement GB/T 14926.47-2001 "experimental animal Shigella detection method". The main technical differences between this part and GB/T 14926.47-2001 are as follows. a) the GB/T 14926.47-2001 "experimental animal Shigella detection method" part of the content has been revised and increased; b) "5.2GN enrichment solution" was added to the original standard "5 medium and reagent", and the original standard "5.3S.S agar" was modified to "5.4XLD agar"; c) in the original standard "6 test procedures" increased the "GN enrichment solution (36 ± 1) ℃ culture 6h ~ 8h" this procedure; d) "S.S agar" is modified to "XLD agar" in the original standard "6 test procedure"; e) the original standard "7.2 isolation culture will be inoculated with MacConkey agar and S.S agar plate (36 ± 1) ℃ culture 18 ~ 24h. (36 ± 1) ℃ for 6h ~ 8h, and then transferred to McKenzie Agar And XLD agar plates, incubated at (36 ± 1) ℃ for 18 h ~ 24h "; f) Original standard "7.3.1 Colony characteristics Shigella form a colorless, transparent, rounded, flat or slightly convex on the MacConkey and S.S agar plates From the smooth, moist, edge neat, about 2mm in diameter colony. Shigella Shigella colony is generally larger, translucent, sometimes Resulting in flattened, irregular edges of the rough colonies. "Modified as" 7.3.1 Colony characteristics Shigella in MacConkey agar plate The formation of colorless, convex, diameter 2mm ~ 3mm colony in the XLD agar plate to form a red, smooth, diameter 1mm ~ 2mm colonies. Shigella dysentery type 1 colonies formed on the above two media were compared to other Shigella colonies To be small "; g) the original standard "7.3.5 Table 1 Shigella biochemical reaction" in the "seven leaves of decarboxylase" modified to "aescinate." This part of the National Laboratory Animal Standardization Technical Committee proposed and centralized. This part is drafted by the National Laboratory for Laboratory Animal Standardization. This part of the main drafters. Huang Ren. This part of the previous version of the standard to replace the release of the situation. --- GB/T 14926.47-2001. Laboratory animal Shigella detection method

1 Scope

This part of GB/T 14926 specifies the method of detection of Shigella in laboratory animals. This part applies to the detection of Shigella spp.

2 normative reference documents

The terms of the following documents are the terms of this part by reference to this part of ISO /IEC 1540. All references to the date , All subsequent amendments (excluding corrigenda) or revisions do not apply to this part, however, encouragement under this section The parties to the agreement study whether the latest version of these documents can be used. Any reference to a document that is not dated, the latest version of which applies to this section. GB/T 14926.42 Bacteriological examination of experimental animals GB/T 14926.43 Laboratory animals Bacteriological detection Staining methods, media and reagents

3 principle

Shigella is a gram-negative gram-negative bacteria and has specific growth and biochemical characteristics on medium such as trisaccharide iron or disaccharide iron. Identification of Shigella by selective culture, biochemistry and serum test.

4 major equipment and materials

Ordinary incubator.

5 medium and reagents

5.1 Cary-Blair delivery medium. composition and preparation methods see GB/T 14926.43. 5.2 GN enrichment liquid. composition and preparation methods see GB/T 14926.43. 5.3 MacConkey agar. composition and preparation methods see GB/T 14926.43. 5.4 XLD agar. composition and preparation methods see GB/T 14926.43. 5.5 grams of disaccharide iron agar. composition and preparation methods see GB/T 14926.43. 5.6 trisaccharide agar. composition and preparation methods see GB/T 14926.43. 5.7 Citrus Citrate Medium. Ingredients and methods of preparation see GB/T 14926.43. 5.8 amino acid decarboxylase test medium. composition and preparation methods see GB/T 14926.43. 5.9 urea agar. composition and preparation methods see GB/T 14926.43. 5.10 semi-solid agar. composition and preparation methods see GB/T 14926.43. 5.11 sugar fermentation medium. composition and preparation methods see GB/T 14926.43. 5.12 potassium cyanide growth medium. composition and preparation methods see GB/T 14926.43. 5.13 glucose ammonium medium. composition and preparation methods see GB/T 14926.43. 5.14 Shigella multivalent diagnosis of serum.

6 testing procedures

Figure 1 Test procedure

7 operating procedures

7.1 Sampling Take the stool or anal swab specimens, see GB/T 14926.42 for sampling methods. 7.2 culture and culture (36 ± 1) ℃ was incubated for 6h ~ 8h, and the mixture was inoculated with MacConkey agar and XLD agar plate. 1) ℃ for 18h ~ 24h. 7.3 Identification 7.3.1 Colony characteristics Shigella in the McKenzie agar plate on the formation of colorless, convex, diameter 2mm ~ 3mm colony in the XLD agar plate to form red Color, smooth, 1mm ~ 2mm in diameter colonies. Shigella dysentery type 1 in the above two kinds of bacteria on the formation of colonies than other Shiga Bacterial colonies to be small. 7.3.2 Cell characteristics Gram-negative bacilli, no flagellum, no spores. 7.3.3 Culture characteristics (36 ± 1) ℃ for 18h ~ 24h, the slope does not produce acid, the bottom of the acid does not produce gas, Hydrogen sulfide negative. 7.3.4 Dynamic semi-solid test negative. 7.3.5 Biochemical characteristics of the bacteria See Table 1. Table 1 Shigella biochemical reactions Biochemical test project to determine the results Glucosamine - Aescinate - Ornithine decarboxylase a - KCN growth - Biochemical test project to determine the results Lysine decarboxylase - Urea - Citrus Citrate - Salicin - Note. "+" indicates positive, "-" indicates negative. aC group 13 and D group were positive. 7.3.6 serum slide agglutination test positive.

8 results report

Those who meet the above test results to make a positive report, do not meet the negative report. Biochemical reaction does not meet the strains, even if Can be associated with a certain Shigella type of serum agglutination, still can not be identified as Shigella genus culture.

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